Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
100 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
100 µg Transfection-Ready DNA Clear filter

27,469 results

Showing: 5401 - 5420 of 27469 results

  1. TRUPATH Triple Ga12

    Plasmid
    #196061
    Purpose
    Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAlpha12-RLuc8 (GNA12 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    196061-plasmid-map-sequence-id-385874

    Enlarge

  2. TRUPATH Triple Gai2

    Plasmid
    #196049
    Purpose
    Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAlphai2-RLuc8 (GNAI2 Human)
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    196049-plasmid-map-sequence-id-385863

    Enlarge

  3. EGFP-p300

    Plasmid
    #191764
    Purpose
    Expression of EGFP fused to core p300 histone acetyltransferase
    Depositor
    Daniel Gerlich
    Publication
    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FRB-EGFP-p300 core (EP300 Human)
    Tags
    EGFP (N-terminal of p300 core) and FRB (N-termina…
    Expression
    Bacterial and Mammalian
    Promoter
    EF1alpha
    Available since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    191764-plasmid-map-sequence-id-383841

    Enlarge

  5. pCMV-eNme2-C.NR

    Plasmid
    #185672
    Purpose
    Expresses nuclease active eNme2-C.NR in mammalian cells
    Depositor
    David Liu
    Publication
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eNme2-C.NR nuclease
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    eNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…
    Available since
    Sept. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    185672-plasmid-map-sequence-id-364078

    Enlarge

  8. pCMV-CD63-L7Ae

    Plasmid
    #180503
    Purpose
    Human CD63-L7Ae fusion protein
    Depositor
    Masaharu Somiya
    Publication
    Somiya et al bioRxiv 2022.01.25.477620
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Human CD63-L7Ae fusion protein
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    180503-plasmid-map-sequence-id-357065

    Enlarge

  9. DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

    Plasmid
    #176836
    Purpose
    Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.
    Depositor
    Rudolf Jaenisch
    Publication
    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
    Use
    Unspecified
    Mutation
    See Depositor Comments Below
    Promoter
    Tet operator (tetracycline inducible expression)
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    176836-plasmid-map-sequence-id-346773

    Enlarge

  11. PGK plasmid

    Plasmid
    #169744
    Purpose
    Unregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.
    Depositor
    Francois St-Pierre
    Publication
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    PGK promoter
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    169744-plasmid-map-sequence-id-334452

    Enlarge

  12. SpyCas9 PE2*

    Plasmid
    #169850
    Purpose
    Mammalian Expression, SpyCas9 prime editor, optimized NLS
    Depositor
    Scot Wolfe
    Publication
    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9 PE*
    Tags
    bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    June 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    169850-plasmid-map-sequence-id-332905

    Enlarge

  13. pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX

    Plasmid
    #167583
    Purpose
    Express the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cells
    Depositor
    Yulong Li
    Publication
    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    167583-plasmid-map-sequence-id-330893

    Enlarge

  14. pCAG mito-mEos2

    Plasmid
    #168515
    Purpose
    Expression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rate
    Depositor
    Franck Polleux
    Publication
    Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mito-mEos2
    Tags
    mEos2
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    168515-plasmid-map-sequence-id-329879

    Enlarge

  15. pUCM-CLYBL-NGN2-BRN3A

    Plasmid
    #165597
    Purpose
    Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.
    Depositor
    Carsten Bönnemann
    Publication
    Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    BRN3A (POU4F1 Human)
    EGFP
    Puromycin N-acetyl transferase
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Expression
    Mammalian
    Promoter
    CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…
    Available since
    March 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165597-plasmid-map-sequence-id-326012

    Enlarge

Showing: 5401 - 5420 of 27469 results