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27,467 results

Showing: 8041 - 8060 of 27467 results

  1. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197026
    Purpose
    Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized enOsCas12f1
    Expression
    Mammalian
    Mutation
    D52R / T132R
    Promoter
    CBh, CMV, hU6
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    197026-plasmid-map-sequence-id-394112

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  4. pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA

    Plasmid
    #197031
    Purpose
    Vector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized denOsCas12f1-VPR
    Expression
    Mammalian
    Mutation
    D52R / T132R / D228A / D406A
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197031-plasmid-map-sequence-id-388973

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  5. pCDNA3.1_WiChR_TS_mScarlet_ER

    Plasmid
    #195190
    Purpose
    High efficient, K+ selective channelrhodopsin for the inhibition of excitable cells
    Depositor
    Peter Hegemann, Johannes Vierock
    Publication
    Vierock et al Sci Adv. 2022 Nov 16:eadd7729.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Wobblia lunata inhibitory Channelrhodopsin
    Tags
    ER-export sequence, Kir2.1 trafficking motif, and…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    195190-plasmid-map-sequence-id-390435

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  6. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3

    Plasmid
    #189755
    Purpose
    Produces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysis
    Depositor
    Dorus Gadella
    Publication
    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mTurquoise2_spatial-linker-P2A_mScarlet-I3
    Tags
    6xHis
    Expression
    Bacterial and Mammalian
    Promoter
    CMV & Rha
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    189755-plasmid-map-sequence-id-385774

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  8. pFB2D

    Plasmid
    #195286
    Purpose
    Bicistronic DTR-EGFP fusion and Puromycin resistance for positive/negative selection
    Depositor
    Scott Lowe
    Publication
    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DTR EGFP 2A Puro
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    195286-plasmid-map-sequence-id-382886

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  9. pMax_ScaI_BFP

    Plasmid
    #177824
    Purpose
    Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).
    Depositor
    Zachary Nagel, Leona Samson
    Publication
    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tagBFP
    Expression
    Mammalian
    Mutation
    5' inserted ScaI RE cut site
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    177824-plasmid-map-sequence-id-348950

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  10. T7-VEE-mCherry

    Plasmid
    #193355
    Purpose
    Self-replicating RNA vector expressing mCherry fluorescent protein + PuroR
    Depositor
    Hans Schöler
    Publication
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Promoter
    T7
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    193355-plasmid-map-sequence-id-379856

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  14. Rtn4b-GFP

    Plasmid
    #186952
    Purpose
    Expresses Rtn4b connected with C-terminus AcGFP1
    Depositor
    Ke Xu
    Publication
    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RTN4B (RTN4 Human)
    Tags
    AcGFP1
    Expression
    Mammalian
    Promoter
    CMV Promoter
    Available since
    Sept. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    186952-plasmid-map-sequence-id-494680

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  17. CMV mRuby3-Synapsin1a

    Plasmid
    #187896
    Purpose
    For labeling pre-synaptic terminals
    Depositor
    Timothy Ryan
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Synapsin1a
    Tags
    mRuby3
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    187896-plasmid-map-sequence-id-394752

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  19. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

    Plasmid
    #185913
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185913-plasmid-map-sequence-id-367365

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  20. pcDNA3_mScarlet-LAMP1

    Plasmid
    #185138
    Purpose
    Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime
    Depositor
    Andrew York
    Publication
    Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lysosome-associated membrane glycoprotein 1 (Lamp1 Synthetic, Rat)
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-21 of LAMP1
    Promoter
    CMV
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    185138-plasmid-map-sequence-id-366475

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Showing: 8041 - 8060 of 27467 results