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27,467 results

Showing: 8761 - 8780 of 27467 results

  1. NanoLuc-PM-Venus

    Plasmid
    #164786
    Purpose
    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein
    Depositor
    Laszlo Hunyady
    Publication
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Oplophorus gracilirostris)
    Tags
    NanoLuc and mVenus
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164786-plasmid-map-sequence-id-326679

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  3. pcDNA3.1-GGamma11-GFP2

    Plasmid
    #166780
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGamma11-GFP2 (GNG11 Synthetic, Human)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    166780-plasmid-map-sequence-id-331008

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  4. pCS2+ GCamp6-3NLS

    Plasmid
    #165401
    Purpose
    Expresses a nuclear localized genetically encoded calcium reporter.
    Depositor
    Philipp Niethammer
    Publication
    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nuclear Localization Signal
    Use
    Avian, xenopus, zebrafish
    Tags
    GCamp6
    Expression
    Mammalian
    Promoter
    SP6
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    165401-plasmid-map-sequence-id-325983

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  5. pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX

    Plasmid
    #164612
    Purpose
    Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in mammalian cells
    Depositor
    Yulong Li
    Publication
    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Endocannabinoid non-responsive sensor GRAB_eCBmut
    Expression
    Mammalian
    Mutation
    S383T, F177A
    Promoter
    CMV
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164612-plasmid-map-sequence-id-325166

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  6. pJYDNp

    Plasmid
    #162451
    Purpose
    Positive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.
    Depositor
    Gideon Schreiber
    Publication
    Zahradník et al bioRxiv Preprint 2020
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Aga2p-eUnaG2
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA and myc
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162451-plasmid-map-sequence-id-321446

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  7. AICSDP-117:POLR2A-mEGFP

    Plasmid
    #164500
    Purpose
    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    POLR2A Homology Arms with mEGFP-linker (POLR2A Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Feb. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    164500-plasmid-map-sequence-id-324900

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  8. pSpdCas9-huTET1CD-T2A-GFP (PX458)

    Plasmid
    #129025
    Purpose
    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-huTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129025-plasmid-map-sequence-id-454365

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  9. ER-mGreenLantern

    Plasmid
    #164463
    Purpose
    Localization and retention of mGreenLantern fluorescent protein in the ER
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mGreenLantern
    Tags
    Calreticulin and KDEL
    Expression
    Mammalian
    Mutation
    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…
    Promoter
    CMV
    Available since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    164463-plasmid-map-sequence-id-322370

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  11. pCMV-ABE8eWQ

    Plasmid
    #161815
    Purpose
    Expresses ABE8eWQ in mammalian cells
    Depositor
    Sangsu Bae
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    161815-plasmid-map-sequence-id-320654

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  13. GEF-H1 FLARE191.pa

    Plasmid
    #134931
    Purpose
    GEF-H1 biosensor that has GEF activity.
    Depositor
    Klaus Hahn
    Publication
    Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
    Tags
    None.
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and T7
    Available since
    Dec. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    134931-plasmid-map-sequence-id-319656

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  15. pTRE3G-iRFP-B1-timestamp_A

    Plasmid
    #159924
    Purpose
    Timestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.
    Depositor
    Fei Chen
    Publication
    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iRFP-Timestamp A
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    159924-plasmid-map-sequence-id-318472

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  16. pJ609-dCas9-KR-puro

    Plasmid
    #158478
    Purpose
    Express nuclease dead Cas9 fused to KillerRed chromophore in mammalian cells
    Depositor
    Brendan Price
    Publication
    House et al PLoS One. 2020 Dec 17;15(12):e0237759. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-KR
    Use
    CRISPR
    Tags
    KillerRed
    Expression
    Mammalian
    Mutation
    Cas9m4 (D10A+D839A+H840A+N863A)
    Promoter
    CMV
    Available since
    Nov. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    158478-plasmid-map-sequence-id-317143

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  18. pCD5-PR8-TEV-sfGFP-twinStrep

    Plasmid
    #157933
    Purpose
    Expresses influenza A virus trimeric hemagglutinin fused to a sfGFP
    Depositor
    Robert de Vries
    Publication
    Nemanichvili et al J Mol Biol. 2019 Feb 15;431(4):842-856.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    A/PuertoRico/8/34 hemagglutinin (HA Synthetic)
    Tags
    sfGFP & TwinStrep
    Expression
    Mammalian
    Mutation
    E190D
    Promoter
    CMV
    Available since
    Oct. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    157933-plasmid-map-sequence-id-315089

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  19. pEGFP-NUP188

    Plasmid
    #159893
    Purpose
    Expresses NUP188 in mammalian cells
    Depositor
    Kozo Tanaka
    Publication
    Itoh et al Cancer Sci. 2013 Jul;104(7):871-9. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NUP188 (NUP188 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    To avoid NotI site, first three amino acids are d…
    Promoter
    CMV
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159893-plasmid-map-sequence-id-313971

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  20. DD-DamV133A-tdTomato-LMNB1

    Plasmid
    #159604
    Purpose
    Shield-1 inducible transient mammalian expression of Dam-tdTomato-LMNB1 for DamID, containing the V133A mutant of Dam and a fused tdTomato to monitor fusion protein localization and expression levels.
    Depositor
    Aaron Streets
    Publication
    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DD-DamV133A-tdTomato-LMNB1
    Expression
    Mammalian
    Mutation
    Dam V133A mutation
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159604-plasmid-map-sequence-id-311954

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Showing: 8761 - 8780 of 27467 results