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27,466 results

Showing: 11281 - 11300 of 27466 results

  1. pmiHiFiCas9

    Plasmid
    #164832
    Purpose
    miHiFiCas9 expressing plasmid
    Depositor
    Eugene Chen
    Publication
    Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miHiFiCas9 (contains Brex27 at sequence 5031-5138).
    Use
    CRISPR
    Tags
    FLAG and NLS
    Expression
    Mammalian
    Mutation
    change R 691 to A
    Promoter
    CMV
    Available since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    164832-plasmid-map-sequence-id-329428

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  3. TC1316

    Plasmid
    #164883
    Purpose
    CMV-bpNLS-dCas13d-bpNLS-ADARdd-mCherry, ADARdd embeded in loop3
    Depositor
    Tian Chi
    Publication
    Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dcas13d-ADARdd
    Expression
    Mammalian
    Mutation
    dCas13d L560-G586 deleted
    Promoter
    CMV, T7
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    164883-plasmid-map-sequence-id-325957

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  6. pcDNA™5/FRT/CMV_promoter-Cas9-E2A-mRFP

    Plasmid
    #164131
    Purpose
    Expresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)
    Depositor
    Hyongbum Kim
    Publication
    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 (cas9 Synthetic)
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    164131-plasmid-map-sequence-id-323775

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  7. Human a3 nicotinic receptor subunit EM construct

    Plasmid
    #163960
    Purpose
    pEZT-BM vector with alpha3 gene after CMV promoter, published EM construct
    Depositor
    Ryan Hibbs
    Publication
    Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Human alpha3 nicotinic acetylcholine receptor subunit EM construct
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    apocytochrome b(562)RIL (BRIL)
    Expression
    Mammalian
    Mutation
    Asn348-Ser402 replaced with apocytochrome b(562)R…
    Promoter
    CMV
    Available since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163960-plasmid-map-sequence-id-323882

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  9. pEGFP-C1 PA-PLA1 (EGFP-PA-PLA1)

    Plasmid
    #162880
    Purpose
    Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with EGFP
    Depositor
    Frederic Meunier
    Publication
    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    162880-plasmid-map-sequence-id-321988

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  11. AAVS1_puro_CAG_HexaPro

    Plasmid
    #164077
    Purpose
    Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus
    Depositor
    Yannick Doyon
    Publication
    Rivest et al protocols.io 2021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)
    Use
    CRISPR
    Tags
    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CAG
    Available since
    Jan. 4, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    164077-plasmid-map-sequence-id-320680

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  14. pCDNA3.1 hSCN5AdeltaSIV GFP Nter

    Plasmid
    #145770
    Purpose
    expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells
    Depositor
    Hugues Abriel
    Publication
    Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Stop codon at Serine 2013
    Promoter
    CMV
    Available since
    Oct. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    145770-plasmid-map-sequence-id-315499

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  15. INS-2A-luciferase-2A-CD19-GFP

    Plasmid
    #159349
    Purpose
    Human INS targeting vector for knockin of 2A separated luciferase, humand CD19, and GFP. Targeted cells will be puromycin resistant.
    Depositor
    Rudolf Jaenisch
    Publication
    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Luciferase-2A-human_CD19-2A-GFP
    Luciferase-2A-human_CD19-2A-GFP (CD19 Human)
    Use
    Ins locus knockin donor vector
    Available since
    Oct. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    159349-plasmid-map-sequence-id-313044

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  16. hHS1-M7A,H102A

    Plasmid
    #159171
    Purpose
    Human codon-optimized cytosolic labile heme reporter
    Depositor
    Amit Reddi
    Publication
    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hHS1-M7A,H102A
    Expression
    Mammalian
    Mutation
    Mutated both Met 7 and His 102 of the cyt b562 mo…
    Promoter
    CMV
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    159171-plasmid-map-sequence-id-311544

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Showing: 11281 - 11300 of 27466 results