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27,006 results

Showing: 4901 - 4920 of 27006 results

  7. pCMV-dCas13-inactive M3M14nes

    Plasmid
    #157855
    Purpose
    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant
    Depositor
    David Liu
    Publication
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A; METTL3 D395A
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    157855-plasmid-map-sequence-id-307628

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  8. pcDNA3.1.Gαs-LgB113

    Plasmid
    #134362
    Purpose
    Nanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gαs-LgB113 (GNAS Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134362-plasmid-map-sequence-id-306402

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  9. RA-Sec61β

    Plasmid
    #153978
    Purpose
    Dimerization dependent fluorescent protein RA domain fused to ER protein Sec61β
    Depositor
    Gia Voeltz
    Publication
    Lee et al Science. 2020 Jan 31;367(6477). pii: 367
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sec61 Transolocon Subunit Beta (SEC61B Human)
    Tags
    pAcGFP-C1
    Expression
    Mammalian
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    153978-plasmid-map-sequence-id-306136

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  10. pF4Ag NanoLuc (ATG-42)

    Plasmid
    #137777
    Purpose
    Expresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)
    Depositor
    Lance Encell
    Publication
    Hall et al ACS Chem Biol. 2012 Nov 16;7(11):1848-57
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NanoLuc (Nluc)
    Expression
    Bacterial and Mammalian
    Mutation
    NanoLuc contains 16 amino acid changes compared t…
    Promoter
    T7 and CMV
    Available since
    March 27, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    137777-plasmid-map-sequence-id-276018

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  15. pEBTet_NADP-Snifit-cyto

    Plasmid
    #113917
    Purpose
    Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells
    Depositor
    Kai Johnsson
    Publication
    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NADP-Snifit
    Expression
    Mammalian
    Promoter
    CMV TetO2
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    113917-plasmid-map-sequence-id-215100

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  16. EasyFusion Halo-mAID

    Plasmid
    #112852
    Purpose
    General donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous protein
    Depositor
    Janet Rossant
    Publication
    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Halo-mAID
    Use
    Synthetic Biology
    Promoter
    No
    Available since
    July 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    112852-plasmid-map-sequence-id-217036

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  19. AICSDP-47: CTNNB1-mEGFP

    Plasmid
    #109119
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human CTNNB1
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CTNNB1 Homology Arms with mEGFP-linker (CTNNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    May 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    109119-plasmid-map-sequence-id-209765

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  20. SID4x-dCas9-KRAB

    Plasmid
    #106399
    Purpose
    Expresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domain
    Depositor
    Jason Gertz
    Publication
    Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SID4x (MXD1 Human)
    KRAB (ZNF10 Human)
    Tags
    FLAG, HA, and NLS
    Expression
    Mammalian
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    106399-plasmid-map-sequence-id-207594

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Showing: 4901 - 4920 of 27006 results