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30,870 results

Showing: 5221 - 5240 of 30870 results

  2. dead Sa Cas9

    Plasmid
    #138162
    Purpose
    For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSaCas9
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    D10A + N580A
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138162-plasmid-map-sequence-id-272344

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  9. pEBTet_NAD-Snifit-mito

    Plasmid
    #113916
    Purpose
    Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells
    Depositor
    Kai Johnsson
    Publication
    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NAD-Snifit
    Expression
    Mammalian
    Promoter
    CMV TetO2
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113916-plasmid-map-sequence-id-222706

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  11. pcDNA 3.1 SIRT1-FL

    Plasmid
    #105670
    Purpose
    Mammalian expression construct of mouse SIRT1 full length isoform
    Depositor
    Ullas Kolthur-Seetharam
    Publication
    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SIRT1 (Sirt1 Mouse)
    Expression
    Mammalian
    Mutation
    Synonymous base changes not affecting the protein…
    Promoter
    CMV
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105670-plasmid-map-sequence-id-207902

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  12. xCas9(3.6)-BE3

    Plasmid
    #108385
    Purpose
    Mammalian expression vector for xCas9(3.6)-BE3
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9(3.6)-BE3
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    108385-plasmid-map-sequence-id-207181

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  13. VPR-ps-dSpCas9

    Plasmid
    #102855
    Purpose
    A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains
    Depositor
    Michael Lin
    Publication
    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSpCas9, pdDronpa1.2
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS and VP64-p64-Rta transcription activator
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    102855-plasmid-map-sequence-id-199006

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  15. Ezh2[SET]-dCas9

    Plasmid
    #100087
    Purpose
    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ezh2[SET] (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Ezh2[SET] includes aa 482-746
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100087-plasmid-map-sequence-id-197556

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  16. pSNAP-CaaX Control Plasmid

    Plasmid
    #101133
    Purpose
    Expression of SNAP-Tag and CaaX box (Farnesylation Signal) in Mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CaaX Box
    Tags
    SNAP-tag (SNAP26m)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101133-plasmid-map-sequence-id-194147

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  17. pMOD_B2303

    Plasmid
    #91068
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    Use
    CRISPR
    Promoter
    CmYLCV
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91068-plasmid-map-sequence-id-181874

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  19. pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

    Plasmid
    #92390
    Purpose
    expresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cells
    Depositor
    Hyungbae Kwon
    Publication
    Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92390-plasmid-map-sequence-id-181393

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  20. MCS+

    Plasmid
    #90412
    Purpose
    A FRET sensor that report the electric potential at the inner leaflet of cell plasma membrane
    Depositor
    Katharina Gaus
    Publication
    Ma et al Nat Biotechnol. 2017 Mar 13. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lck10+ Venus+mcherry+ strech of positively charged amino acids
    Expression
    Mammalian
    Available since
    May 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90412-plasmid-map-sequence-id-178958

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Showing: 5221 - 5240 of 30870 results