Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

30,881 results

Showing: 5781 - 5800 of 30881 results

  1. pcDNA3.1.Gαi3-LgB91

    Plasmid
    #134359
    Purpose
    Nanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gαi3-LgB91 (GNAI3 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134359-plasmid-map-sequence-id-306392

    Enlarge

  3. pcDNA3.1-GGamma1-GFP2

    Plasmid
    #140989
    Purpose
    Encodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGamma1-GFP2 (GNGT1 Human)
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140989-plasmid-map-sequence-id-302012

    Enlarge

  8. pDONR221_SLC2A1

    Plasmid
    #132103
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Publication
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SLC2A1 (SLC2A1 Human)
    Expression
    Mammalian
    Available since
    Dec. 3, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    132103-plasmid-map-sequence-id-265224

    Enlarge

  11. pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)

    Plasmid
    #85798
    Purpose
    donorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression system
    Depositor
    Roland Friedel
    Publication
    Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PPP1R12C (PPP1R12C Human)
    Use
    Human targeting
    Mutation
    homology arms for knock-in into first intron
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85798-plasmid-map-sequence-id-516551

    Enlarge

  12. TNFR2-GFP

    Plasmid
    #111207
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TNFRSF1B (TNFRSF1B Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    S175P, D306G
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111207-plasmid-map-sequence-id-213090

    Enlarge

  14. pGW1-mCherry-EGFP-PIM

    Plasmid
    #111759
    Purpose
    High expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition
    Depositor
    Lukas Kapitein
    Publication
    Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
    Expression
    Mammalian
    Mutation
    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …
    Promoter
    CMV
    Available since
    Oct. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111759-plasmid-map-sequence-id-222507

    Enlarge

  15. pEBTet_NADP-Snifit-mito

    Plasmid
    #113919
    Purpose
    Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells
    Depositor
    Kai Johnsson
    Publication
    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NADP-Snifit
    Expression
    Mammalian
    Promoter
    CMV TetO2
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    113919-plasmid-map-sequence-id-215103

    Enlarge

  16. AICSDP-29: LMNB1-mTagRFP-T

    Plasmid
    #114403
    Purpose
    Homology arms and mTagRFPT-linker sequence for N-terminus tagging of human LMNB1
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNB1 Homology Arms with mTagRFPT-linker (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    mTagRFPT-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    114403-plasmid-map-sequence-id-220813

    Enlarge

Showing: 5781 - 5800 of 30881 results