27,006 results
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Plasmid#182144PurposeTo insert attP attachment site at AAVS1 in human cells via twinPEDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAAVS1_B3903c_attP pegRNA
ExpressionMammalianPromoterhU6Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-gRNA-AAVS1_A3786c_attP
Plasmid#182143PurposeTo insert attP attachment site at AAVS1 in human cells via twinPEDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertA3786c-attP pegRNA
ExpressionMammalianPromoterhU6Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-mCherry-EEA1
Plasmid#174452PurposeExpress mCherry with early endosome-targeting sequence in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry with early endosome-targeting sequence
Tagsearly endosomal antigen 1 (EEA1)ExpressionMammalianPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Rogi1-CMV-LAMB3-T2A-GFP-bGH
Plasmid#179864PurposeTargets Rogi1 genomic region with HDR and expresses LAMB3-T2A-GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLAMB3-T2A-GFP
Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_IRF1
Plasmid#167760PurposeExpresses IRF1 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIRF1 (IRF1 Human)
TagsMACExpressionMammalianMutationno mutationsAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
ELP-TEMP/pcDNA3.1(-)
Plasmid#178434PurposeGenetically encoded ratiometric fluorescent thermometerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertELP-TEMP
TagsNo tagExpressionMammalianAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-noPS
Plasmid#177943PurposeTransient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
ExpressionMammalianAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA
Plasmid#171380PurposeExpression vector for encoding a human codon-optimized RfxCas13d driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized RfxCas13d
TagsHAExpressionMammalianPromoterCMV, U6Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAEMXT-eLACCO1.1
Plasmid#167946PurposeFluorescent biosensor for extracellular L-lactateDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD59-eLACCO1.1-GPI
ExpressionMammalianPromoterCMVAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSB-CMV-MCS-Puro dr_cPLA2-mKate2
Plasmid#163844PurposeUsed as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
TagsmKate2ExpressionMammalianPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 H2B-mIFP T2A Mpro (wt)
Plasmid#163079PurposeExpresses wild type Mpro (active) and a near-infrared fluorescent protein mIFP fused to H2BDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertWild type Mpro of SARS-CoV-2 and H2B-mIFP
ExpressionMammalianAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB_SBE4:mScarlet-I-NLS
Plasmid#154961Purposeencodes transcriptional reporter of SMAD2/3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmScarlet
UsePiggybacTagsNLS-PESTExpressionMammalianAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
iOn-CAG∞RFP
Plasmid#154014PurposeVector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposaseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmRFP1
ExpressionMammalianPromoterCAGAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi2-LgB91
Plasmid#134358PurposeNanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGαi2-LgB91 (GNAI2 Human)
TagsHAExpressionBacterial and MammalianPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlpha15-RLuc8
Plasmid#140984PurposeEncodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAlpha15-RLuc8 (GNA15 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlpha13-RLuc8
Plasmid#140986PurposeEncodes a G alpha subunit (GNA13) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAlpha13-RLuc8 (GNA13 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA4/TO/GFP-GIGYF2
Plasmid#141189PurposeExpresses GFP-GIGYF2 in mammalian cells, can be used to make inducible cell lineDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGIGYF2 (GIGYF2 Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceJune 5, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP - FLAG-APEX2- hs Lamin B1
Plasmid#139442PurposeAPEX2-Lamin B1 expression plasmidDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLMNB1 (LMNB1 Human)
Tags3xGGGGS linker (between APEX2 and Lamin B1), APEX…ExpressionMammalianPromoterCMVAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PL-5LTR-RGR(DMD#1)-AmCyan-A
Plasmid#138482PurposeExpresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRibozyme-flanked gRNA and AmCyan
UseCRISPRExpressionMammalianAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP680-tubulin
Plasmid#136575PurposeHuman alpha-tubulin labeling with a near-infrared fluorescent protein miRFP680DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmiRFP680-tubulin
TagsmiRFP680ExpressionMammalianPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only