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30,881 results

Showing: 6141 - 6160 of 30881 results

  2. pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

    Plasmid
    #85072
    Purpose
    Green/red FRET biosensor for RhoA in the GTP locked constitutively active state
    Depositor
    Dorus Gadella
    Publication
    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RhoA Biosensor constitutively active (Q63L) (RHOA Human)
    Tags
    6xHis and mScarlet-i and SGFP2
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Q63L
    Promoter
    CMV
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    85072-plasmid-map-sequence-id-163878

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  6. Lyn11-CIBN-GFP

    Plasmid
    #79572
    Purpose
    expression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP
    Depositor
    Pietro De Camilli, Olof Idevall-Hagren
    Publication
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn11
    Tags
    CIBN (CRY2-binding domain) and EGFP
    Expression
    Mammalian
    Mutation
    G8R (please see depositor comments below)
    Promoter
    CMV
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  7. KRas FAK biosensor

    Plasmid
    #78301
    Purpose
    FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells
    Depositor
    Yingxiao Wang
    Publication
    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KRas FAK biosensor
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. Rlucrarr3-Flash5

    Plasmid
    #74133
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.
    Depositor
    Louis Luttrell
    Publication
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Arrestin3
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  11. cpVenus-Gγ2

    Plasmid
    #69626
    Purpose
    The heterotrimeric G-protein subunit, Gγ2, tagged with circular permutated Venus (cp173V).
    Depositor
    Dorus Gadella
    Publication
    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cp173Venus-Ggamma2 (GNG2 Synthetic)
    Tags
    ciruclar permutated Venus (cp173V)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    69626-plasmid-map-sequence-id-123360

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  14. HTR1B-Tango

    Plasmid
    #66405
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HTR1B (HTR1B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66405-plasmid-map-sequence-id-347879

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  15. FFA3-Tango

    Plasmid
    #66282
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FFA3 (FFAR3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  16. ADRB3-Tango

    Plasmid
    #66221
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ADRB3 (ADRB3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. Str-STIM1-NN_ST-SBP-EGFP

    Plasmid
    #65262
    Purpose
    synchronize trafficking of ST from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
    Tags
    EGFP and Streptavidin Binding Protein (SBP)
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 43 of ST
    Promoter
    CMV
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65262-plasmid-map-sequence-id-120697

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  19. HaloTag-G protein gamma-2

    Plasmid
    #67018
    Purpose
    G protein gamma-2 tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HaloTag-G protein gamma-2 (GNG2 Human)
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    67018-plasmid-map-sequence-id-115487

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  20. GFP-CECR2

    Plasmid
    #65385
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CECR2 (CECR2 Human)
    Tags
    EmGFP
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 6141 - 6160 of 30881 results