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27,006 results

Showing: 6181 - 6200 of 27006 results

  1. pC0051-W85X REPAIR targeting guide cloned into pC0043

    Plasmid
    #103867
    Purpose
    Guide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    W85X targeting guide RNA
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103867-plasmid-map-sequence-id-199148

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  2. AICSDP-25:MAP1LC3B-mEGFP

    Plasmid
    #101783
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3B
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    101783-plasmid-map-sequence-id-197671

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  3. dCas9-FOG1[N+C]

    Plasmid
    #100085
    Purpose
    dCas9 fused to two FOG1 peptide [aa 1-45] , one at the N-terminus and one at the C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FOG1(aa 1-45) (ZFPM1 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-FOG1-dCas9-FOG1-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    100085-plasmid-map-sequence-id-197489

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  5. CD8-GFP-FKBP

    Plasmid
    #100751
    Purpose
    Expression of GFP-FKBP at the plasma membrane in mammalian cells. Plasma membrane targeting is via CD8 alpha chain.
    Depositor
    Stephen Royle
    Publication
    Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD8A (CD8A Human)
    Tags
    FKBP and GFP
    Expression
    Mammalian
    Mutation
    Truncated
    Promoter
    CMV
    Available since
    Sept. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    100751-plasmid-map-sequence-id-194049

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  6. pCS2+ H2B-mCherry

    Plasmid
    #99265
    Purpose
    Contains a fusion of human histone H2B to fluorescent protein mCherry in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.
    Depositor
    Philipp Keller
    Publication
    Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Tags
    H2B
    Expression
    Mammalian
    Promoter
    SP6
    Available since
    Sept. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    99265-plasmid-map-sequence-id-192948

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  7. pcDNA3 Flag-humanTRAF3 (1096)

    Plasmid
    #66928
    Purpose
    Mammalian Expression of TRAF3
    Depositor
    Michael Karin
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TRAF3 (TRAF3 Human)
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    66928-plasmid-map-sequence-id-190145

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  10. pMOD_B2515

    Plasmid
    #91072
    Purpose
    Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning
    Use
    CRISPR
    Promoter
    AtU6
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    91072-plasmid-map-sequence-id-181899

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  11. pGP-CMV-GCaMP6-150

    Plasmid
    #91777
    Purpose
    Mammalian expression of low affinity calcium-sensing fluorescent protein
    Depositor
    Douglas Kim, GENIE Project
    Publication
    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GCaMP6-150
    Tags
    6xHis, T7 epitope, and Xpress tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    91777-plasmid-map-sequence-id-178990

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  12. AICSDP-24:MYH10-mEGFP

    Plasmid
    #87428
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10
    Depositor
    Allen Institute for Cell Science
    Publication
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MYH10 Homology Arms with mEGFP-linker (MYH10 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    May 2, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87428-plasmid-map-sequence-id-177429

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  13. pTetON-DeAct-GS1

    Plasmid
    #89454
    Purpose
    Tet/Dox-inducible expression of EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells
    Depositor
    Brad Zuchero
    Publication
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DeAct-GS1 (GSN Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Truncation spanning cytoplasmic Gsn amino acids 5…
    Promoter
    TRE3G
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    89454-plasmid-map-sequence-id-176821

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  16. pTRE-Tight-BI-Gl NORM-LacZA-TER-LacZB

    Plasmid
    #86194
    Purpose
    beta Globin NMD construct with and without PTC under Bi-directional TET on promoter
    Depositor
    Robert Singer
    Publication
    Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    beta Globin (HBB, Hbb Human, Mouse)
    Use
    Tet on
    Expression
    Mammalian
    Mutation
    39TER in beta Globin in MCS II
    Promoter
    Tet on
    Available since
    Jan. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    86194-plasmid-map-sequence-id-169938

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  17. ITSN-mcherry-sspB

    Plasmid
    #85220
    Purpose
    Role of Cdc-42 selective GEF domain from ITSN in immune cell migration
    Depositor
    Narasimhan Gautam
    Publication
    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ITSN1(1225-1575) (ITSN1 Human)
    mcherry
    sspB
    Tags
    NA
    Expression
    Mammalian
    Mutation
    R73Q
    Promoter
    CMV
    Available since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    85220-plasmid-map-sequence-id-169605

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  19. pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

    Plasmid
    #84247
    Purpose
    Expresses direct fusion VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    VPR-tagBFP-Sp dCas9
    Puro
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), HA tag, VPR, and tagBFP
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    84247-plasmid-map-sequence-id-160890

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Showing: 6181 - 6200 of 27006 results