30,881 results
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Plasmid#129093PurposeKI CreER with a puromycin/mRuby selection cassette into human LGR5 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCreERT2 and LGR5 homology arms (LGR5 Human)
UseHomologous recombination donor plasmidAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCIneoGFP-BMI1
Plasmid#128328PurposeMammalian expression vector of GFP-tagged human BMI1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBMI1 (BMI1 Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1+/-FLI1-C‐(K)‐DYK
Plasmid#129497PurposeMammalian expression of human FLI1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLI1 (FLI1 Human)
TagsFlagExpressionMammalianPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-TRIM28deltaPB
Plasmid#124959PurposeMammalian expression of Human TRIM28 with deleted PHD and BROMO domainDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthTRIM28 (TRIM28 Human)
TagsHAExpressionMammalianMutationDeletion of PHD and Bromo DomainAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBT120b
Plasmid#122604PurposeBE PACE selection, express gIII, luxAB and degron-GTCCA guide RNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgIII, luxAB, guide RNA
UseSynthetic BiologyAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT376
Plasmid#125617PurposeExpresses BE4max-NG in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBE4max-NG
ExpressionMammalianAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Influenza-FPV-HA-mEGFP
Plasmid#127810PurposeExpression vector Influenza A virus FPV (H7N1) hemagglutinin mEGFP fusion protein, plasma membrane localizationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertInfluenza A virus FPV (H7N1) hemagglutinin mEGFP fusion
ExpressionMammalianAvailable sinceJune 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-TetON-dual-SunTag-Hygro
Plasmid#121119PurposeEndogenous gene activation with the SunTag CRISPR activation systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-GCN4x10_scFv-VP64
UseCRISPRExpressionMammalianPromoterTet-ONAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ArchT-BFP2-TSERex
Plasmid#123312PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertArchT-BFP2
ExpressionMammalianPromoterCAGAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
cycLuc_TEVS
Plasmid#119207PurposeCircularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcyclic luciferase with TEVs cleavage site
UseLuciferaseTags6xHis and AU1 tagExpressionMammalianPromoterCMVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9(BB)-T2A-HygR
Plasmid#118153PurposeSpCas9 expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthSpCas9-T2A-HygR
UseCRISPRTags3XFLAGExpressionMammalianPromoterCAG and U6Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330-sgAAVS1
Plasmid#85802PurposeCas9/CRISPR vector for cut at human AAVS1 gene intron1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPPP1R12C (PPP1R12C Human)
ExpressionMammalianAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK365 (Tet-OsTIR1-loxP-PURO-loxP)
Plasmid#121185PurposeOsTIR1 introductionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTet-OsTIR1-loxP-PURO-loxP
UseCRISPRMutationOsTIR1 codon optimized for human cellsAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
K9L mutant of H3K9me3 biosensor
Plasmid#120807PurposeFRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capabilityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
ExpressionMammalianMutationlysine 9 is mutated to leucine 9 on histone H3PromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/Envelope
Plasmid#120821PurposeEctopic expression of ZIKV proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEnvelope ZIKV
TagsV5ExpressionMammalianPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sapphireCKAR
Plasmid#118468Purposecp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsapphireCKAR
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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mScarlet-I-PXN
Plasmid#112956PurposeIn vivo visualization of focal adhesions (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPXN
TagsmScarlet-IExpressionMammalianPromoterCMVAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
ITPKA_3xmScarletI
Plasmid#112960PurposeIn vivo visualization of F-actin (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertITPKA
Tags3x mScarlet-IExpressionMammalianPromoterCMVAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by