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30,881 results

Showing: 9021 - 9040 of 30881 results

  1. pY036_ATP1A1_G3_Array

    Plasmid
    #86619
    Purpose
    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86619-plasmid-map-sequence-id-168855

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  6. pC-FLAG-ZCD2

    Plasmid
    #79274
    Purpose
    Mammalian expression of ZCD2 with a C-terminal FLAG tag
    Depositor
    Rita Shiang
    Publication
    Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ZCD2 (CISD2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 10, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pEQ276

    Plasmid
    #83945
    Purpose
    express human cytomegalovirus IE1 and IE2 genes in eukaryotic cells
    Depositor
    Adam Geballe
    Publication
    Biegalke et al Virology. 1991 Jul;183(1):381-5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IE1 and IE2
    Expression
    Mammalian
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  10. pMA-SpCas9-g5

    Plasmid
    #80788
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80788-plasmid-map-sequence-id-162408

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  11. DT12A

    Plasmid
    #80416
    Purpose
    Expresses 12 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats
    Depositor
    Thomas Cooper
    Publication
    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
    Expression
    Mammalian
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80416-plasmid-map-sequence-id-457373

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  13. RP4-7

    Plasmid
    #79811
    Purpose
    Modified broad host range plasmid RP4. Chloramphenicol resistant. Helper plasmid
    Depositor
    Michael Hynes
    Publication
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Chloramphenicol acetyltransferase
    Use
    Helper plasmid for mobilization
    Promoter
    Own promoter
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  17. pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4

    Plasmid
    #78349
    Purpose
    Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.
    Depositor
    Michael Elowitz
    Publication
    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    rTetR
    Expression
    Mammalian
    Promoter
    pEF alpha
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    78349-plasmid-map-sequence-id-146274

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  20. pCB6-X:31HA

    Plasmid
    #74371
    Purpose
    Mammalian expression of wild-type HA from the X:31 strain of influenza virus
    Depositor
    Judith White
    Publication
    Qiao et al Mol Biol Cell. 1999 Aug;10(8):2759-69.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HA
    Expression
    Mammalian
    Available since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 9021 - 9040 of 30881 results