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27,462 results

Showing: 9021 - 9040 of 27462 results

  6. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

    Plasmid
    #226105
    Purpose
    ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)
    Depositor
    Michael Rape
    Publication
    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    atf4 (ATF4 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains uORF1-2 of ATF4, whose stability can ser…
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    226105-plasmid-map-sequence-id-448414

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  7. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Publication
    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

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  11. ISDra2-TnpB-mCherry-U6-ωRNA*

    Plasmid
    #212967
    Purpose
    Expression vector for expression of ISDra2-TnpB driven by chicken β-actin promoter and mCherry driven by CMV promoter
    Depositor
    Chunlong Xu
    Publication
    Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ISDra2-TnpB-T2A-mCherry
    ωRNA*
    Use
    CRISPR
    Tags
    NLS and NLS-FLAG
    Expression
    Mammalian
    Promoter
    CAG, T7 and U6
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    212967-plasmid-map-sequence-id-423077

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  13. SuperClomeleon

    Plasmid
    #203724
    Purpose
    An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride
    Depositor
    George Augustine
    Publication
    Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cerulean linked topaz fluorescent protein
    Expression
    Bacterial and Mammalian
    Mutation
    Cerulean, modified Topaz Fluorescent Protein with…
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    203724-plasmid-map-sequence-id-403640

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  14. pIG-601_NY-ESO-1_TCR_GFP

    Plasmid
    #207482
    Purpose
    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
    Depositor
    Alexander Marson
    Publication
    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP, NY-ESO-1_TCR
    Use
    CRISPR
    Mutation
    Potential silent point mutation in GFP sequence
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    207482-plasmid-map-sequence-id-412258

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  15. pDisplay-GRAB_SST1.0

    Plasmid
    #208664
    Purpose
    Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in mammalian cells
    Depositor
    Yulong Li
    Publication
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    208664-plasmid-map-sequence-id-419238

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Showing: 9021 - 9040 of 27462 results