Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

27,462 results

Showing: 11141 - 11160 of 27462 results

  1. EK0438 SFFV-mCherry-SGc(s70587)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid
    #191163
    Purpose
    Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).
    Depositor
    Xiaojing Gao
    Publication
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry:s70587:EGFP-3xMS2
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    SFFV
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191163-plasmid-map-sequence-id-375409

    Enlarge

  2. EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)

    Plasmid
    #191164
    Purpose
    Similar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequences
    Depositor
    Xiaojing Gao
    Publication
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry:s70587:EGFP (additional cutsites)
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    SFFV
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191164-plasmid-map-sequence-id-375473

    Enlarge

  5. mCherry-p62-Silent_Mutation_WT

    Plasmid
    #187986
    Purpose
    human p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516
    Depositor
    Sascha Martens
    Publication
    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p62 (NUP62 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    silent mutation for resistance to sip62 #5
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    187986-plasmid-map-sequence-id-374441

    Enlarge

  8. pCAGGS SARS-CoV-2 BA.4.6 Spike

    Plasmid
    #190013
    Purpose
    Expresses codon-optimized full length SARS-CoV-2 BA.4.6 Spike
    Depositor
    Marceline Côté
    Publication
    Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 BA.4.6 Spike (S )
    Expression
    Mammalian
    Mutation
    Contains the following mutations: T19I, LPPA24S, …
    Promoter
    chicken β-actin promoter, CMV enhancer
    Available since
    Sept. 7, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    190013-plasmid-map-sequence-id-371149

    Enlarge

Showing: 11141 - 11160 of 27462 results