30,738 results

1. pKM444

   Plasmid

   #108319

   Purpose

   For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Inserts

   Che9C RecT
   Bxb1 Integrase
   Tet Repressor
   

   Expression

   Bacterial

   Available Since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUCP20T-eyfp

   Plasmid

   #78466

   Purpose

   Broad range bacterial fluorescence labeling

   Depositor

   Mariette Barbier

   Article

   Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

   Insert

   eYFP
   

   Expression

   Bacterial

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX_hDDX5_FL_wt

   Plasmid

   #88871

   Purpose

   inducible expression of GST-DDX5 in bacteria

   Depositor

   Dan Littman

   Article

   Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

   Insert

   DDX5 (DDX5 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   Tac

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSF-G1(7FTrp)RS

   Plasmid

   #177310

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al ACS Sens. 2022 Jan 10. doi: 10.1021/acss

   Insert

   tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMC037-HisMS2_PLP_pac

   Plasmid

   #128233

   Purpose

   Expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

   Depositor

   Paul Freemont

   Article

   de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

   Inserts

   Maturation Protein
   Coat Protein Dimer
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 15, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pLPO2

   Plasmid

   #209976

   Purpose

   Contains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   replication origin (SH71_rep)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pDL17

   Plasmid

   #106483

   Purpose

   pDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosome

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

   Inserts

   gam
   bet
   exo
   lacI
   rhaR
   rhaS
   

   Expression

   Bacterial

   Available Since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNCS mHoneydew

   Plasmid

   #91760

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Honeydew (mHoneydew)

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Insert

   monomeric Honeydew
   

   Tags

   no tags

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Human LFA-1 alpha

   Plasmid

   #8630

   Depositor

   Timothy Springer

   Article

   Larson et al J Cell Biol 1989 Feb;108(2):703-12.

   Insert

   LFA1 alpha (ITGAL Human)
   

   Expression

   Bacterial

   Available Since

   June 23, 2005

   Availability

   Academic Institutions and Nonprofits only

10. 6xHis-MBP-huMb3Cas12a

    Plasmid

    #174677

    Purpose

    Expresses 6xHis-MBP tagged Mb3Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huMb3Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-Neq2X7

    Plasmid

    #182366

    Purpose

    Expresses Neq2X7 DNA polymerase

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

    Insert

    Neq2X7 DNA polymerase
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTn7CdrA-gfpC

    Plasmid

    #111616

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Expression

    Bacterial

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid

    #73440

    Purpose

    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    PG6-vioABE-P4A6-vioC-P3A2-vioD
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNMT

    Plasmid

    #42578

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    N-myristoyl tranferase (NMT1 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    two silent mutations T690C and T942C

    Promoter

    T7

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pDM067

    Plasmid

    #216820

    Purpose

    Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for C-terminal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mStayGold2
    

    Tags

    VinnyLinker

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPlacPbuCas13b-gRNA-T-deGFP

    Plasmid

    #184840

    Purpose

    Expression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    single-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119 and lac promoter

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRF-SrpR

    Plasmid

    #49372

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    SrpR repressor
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSL1-GFP

    Plasmid

    #180420

    Purpose

    Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.

    Depositor

    Nancy Moran

    Article

    Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a T7pCONS sfGFP

    Plasmid

    #154462

    Purpose

    pET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Tags

    His-thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSF-T20S

    Plasmid

    #110805

    Purpose

    expresses Thermoplasma acidophilium 20S proteasome in E. Coli

    Depositor

    Yifan Cheng

    Article

    Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

    Inserts

    PsmB
    PsmA
    

    Tags

    tev-His

    Expression

    Bacterial

    Mutation

    N2D and Q2E

    Promoter

    T7 and T7 promoter

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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