29,712 results

1. pBAD/HisD-rsTagRFP

   Plasmid

   #31927

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

   Insert

   rsTagRFP
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

   Promoter

   araB

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVoPo-mCh

   Plasmid

   #187841

   Purpose

   Constitutive mCherry-expression vector for Fusobacterium nucleatum

   Depositor

   Jörg Vogel

   Article

   Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX4T-1-Optimized UBE1L

   Plasmid

   #165099

   Purpose

   ecoli optimized expression of GST-fusion of human Ube1L

   Depositor

   Arthur Haas

   Insert

   UbeE1L (UBA7 Human)
   

   Use

   Tags

   GST from plasmid.

   Expression

   Bacterial

   Mutation

   codon optimized for E. coli expression

   Promoter

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

   Plasmid

   #104394

   Purpose

   Yeast MCP-GFP expression

   Depositor

   Robert Singer, Evelina Tutucci

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   MCP-NLS-2xyeGFP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   MCP fused to NLS(SV40) and 2x yeast codon optimiz…

   Promoter

   CYC1

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBad-mCherry-XL (R125K)

   Plasmid

   #188054

   Purpose

   Expresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteria

   Depositor

   Ralph Jimenez

   Article

   Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

   Insert

   mCherry-XL (R125K)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   differs from mCherry by R125K, W143S, I161V, Q163…

   Promoter

   araBAD

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a(+)-Bst LF-6xHis

   Plasmid

   #159148

   Purpose

   expresses His-tagged large fragment of Bst polymerase

   Depositor

   Andrea Pauli

   Article

   Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

   Insert

   large fragment of Geobacillus stearothermophilus DNA polymerase I
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pVP141

   Plasmid

   #222079

   Purpose

   Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion.

   Depositor

   Wayne Parrott

   Article

   Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

   Insert

   dCas9-PmCDA1, sfGFP and sacB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-MH6-RspCas13d

   Plasmid

   #108305

   Purpose

   Expresses E. coli codon optimized RspCas13d in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

   Insert

   RspCas13d
   

   Use

   CRISPR

   Tags

   mH6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBbE0k-RFP

   Plasmid

   #35365

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-hisMBP-AsCpf1-EC

    Plasmid

    #79007

    Purpose

    Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    AsCpf1
    

    Use

    Tags

    6xHis-MBP-NLS and NLS-HA

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMVV108

    Plasmid

    #215338

    Purpose

    Expresses sfGFP under induction with the Salicylic Acid Marionette Cassette (NahRAM + PSalTTC-sfGFP) with a BBR1 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    PSalTTC

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMBA334

    Plasmid

    #214746

    Purpose

    High copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RiboJ-RBS(21992)-gfpmut3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PET-B-Sniper SpCas9-NLS-6xHis

    Plasmid

    #207377

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Sniper SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F539S, M763I, K890N, amino acids 1005-1013 replac…

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. H6GB1tevP53SS(1-393)

    Plasmid

    #200311

    Purpose

    Bacterial expression of human superstable p53 as His6GB1tev fusion

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    M133L, V203A, N239Y, N268D

    Promoter

    T7

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET3a-OSF-CypA

    Plasmid

    #79039

    Purpose

    Bacterial vector for cloning and propagation in DH5alpha

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OneSTrEP-FLAG- Cyclophilin A (PPIA Human)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-TS2126 RNA Ligase

    Plasmid

    #119941

    Purpose

    Express TS2126 RNA ligase in E. coli

    Depositor

    Takashi Ito

    Article

    Miura et al Nucleic Acids Res. 2019 May 22. pii: 549

    Insert

    TS2126 RNA ligase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBR322-Pdp1_NTD-Cas9

    Plasmid

    #198275

    Purpose

    For loading SpCas9 into PVCs; also contains PVC regulatory genes

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1_NTD-Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLG026 (tmn)

    Plasmid

    #157904

    Purpose

    Expresses the tmn defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    tmn (transmembrane ATPase)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCambia1300-PacI

    Plasmid

    #44183

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVoPo-01

    Plasmid

    #187836

    Purpose

    Backbone for transcriptional reporter system in Fusobacterium nucleatum

    Depositor

    Jörg Vogel

    Article

    Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge