31,663 results
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Plasmid#89950PurposeF-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends.DepositorInsertsTags6*HisExpressionBacterialMutationV222IAvailable SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only
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pCFJ1955
Plasmid#84830PurposeMinimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized tdTomato
ExpressionBacterial and WormPromoterPeft-3Available SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DogCatcher
Plasmid#171772PurposeExpresses DogCatcher protein in bacterial cytoplasmDepositorInsertDogCatcher
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HIV p24
Plasmid#212195PurposeBacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminusDepositorInsertChain A, Capsid protein p24, HIV type 1
TagsPolyhistidineExpressionBacterialAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a-MetRS-His
Plasmid#124115PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL
Plasmid#26096PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneTags6xHis and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMBP-FnCas12a
Plasmid#113432PurposeExpresses 10xHis-MBP fused to FnCas12aDepositorInsertFnCas12a
TagsMBPExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMG046 pETARA-CK1α-His
Plasmid#154074PurposeExpresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coliDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUS252
Plasmid#127674PurposeExpresses fuGFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFP to place in other plasmid backbonesDepositorInsertFree Use GFP (fuGFP)
ExpressionBacterialPromoterlacAvailable SinceJune 24, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
Human Parkin (1-465)
Plasmid#110758PurposeBacterial expression of human ParkinDepositorAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mCherry LIC cloning vector (MBP-mCherry)
Plasmid#29747DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), MBP, TEV cleavage site, a…ExpressionBacterialAvailable SinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAcFRS.2.t1
Plasmid#73544PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAcFRS.2.t1
pAcFRS.2.t1
ExpressionBacterialAvailable SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET His6 NusA TEV LIC cloning vector (2N-T)
Plasmid#29709DepositorTypeEmpty backboneTagsHis6-NusA-TEVExpressionBacterialAvailable SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
GST-beta-arrestin2
Plasmid#36919DepositorAvailable SinceJune 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDIV270
Plasmid#226181PurposeExpresses Cas9 and gRNA targeting KU70 gene in K. phaffii.DepositorInserttRNA-sgRNA-tRNA
UseCRISPRExpressionBacterial and YeastPromoterTEF1p (Komagataella phaffii)Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
2Bc-T MBP_BoMoC(ed)_6xH
Plasmid#186461PurposeRecombinant B. mori truncated R2 RT expressionDepositorInsertB. mori truncated R2 RT
Tags6xHis and MBPExpressionBacterialPromoterT7Available SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
R989-X28-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D
Plasmid#159548PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G12D (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG12DAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHJL4
Plasmid#199379PurposeEncodes dual cistronic cytosolic jGCaMP7f and mScarlet-I optimized for C. elegans expressionDepositorInsertjGCaMP7f::SL2::mScarlet-I
ExpressionBacterialMutationno mutationsAvailable SinceApril 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
R989-X05-566: His6-MBP-tev-G-Hs.KRAS4b(1-188)
Plasmid#159546PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188)DepositorAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET MBP GFP LIC cloning vector (MBP-msfGFP)
Plasmid#29750DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), MBP, SiriusGFP, and TEV c…ExpressionBacterialAvailable SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only