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34,175 results

Showing: 2321 - 2340 of 34175 results

  1. pDule-para-aminoPhe

    Plasmid
    #85502
    Purpose
    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Publication
    Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.
    Insert
    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32T, E107T, D158P, I159L, L162A
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85502-plasmid-map-sequence-id-169860

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  3. pG2K

    Plasmid
    #71742
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Publication
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Expression
    Bacterial
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71742-plasmid-map-sequence-id-516189

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  4. pET-6His-MBP-MpAgo

    Plasmid
    #79249
    Purpose
    This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression
    Depositor
    Jennifer Doudna
    Publication
    Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p
    Insert
    Marinitoga piezophila Argonaute
    Use
    CRISPR
    Tags
    6XHis-tag + MBP + PreScission Cleavage Site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    July 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    79249-plasmid-map-sequence-id-151486

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  7. MAPK1

    Plasmid
    #73248
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAPK1 (MAPK1 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73248-plasmid-map-sequence-id-137205

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  8. pNatA (pACYCduet-naa10-naa15)

    Plasmid
    #72928
    Purpose
    Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker
    Depositor
    Dan Mulvihill
    Publication
    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187
    Inserts
    naa10 (naa10 Synthetic, Fission Yeast)
    naa15 (naa15 Fission Yeast)
    Expression
    Bacterial
    Mutation
    Codon optomised for E.coli expression
    Promoter
    T7
    Available since
    Feb. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    72928-plasmid-map-sequence-id-194851

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  13. pDual-eGFP

    Plasmid
    #63215
    Purpose
    Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Publication
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  14. pSRKBB-empty

    Plasmid
    #59449
    Purpose
    Contains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.
    Depositor
    Claudia Schmidt-dannert
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    modified lac
    Available since
    Sept. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    59449-plasmid-map-sequence-id-521747

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  15. pSEVA3b67Rb

    Plasmid
    #58311
    Purpose
    Shuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.
    Depositor
    Tom Ellis
    Publication
    Wright et al ACS Synth Biol. 2014 May 13.
    Insert
    mRFP1
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    Aug. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    58311-plasmid-map-sequence-id-89824

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  18. pCVL.SSA TLR (Sce)

    Plasmid
    #45576
    Purpose
    Codes for the SSA-TLR with the target site for I-Sce I in a lentiviral backbone
    Depositor
    Andrew Scharenberg
    Publication
    Kuhar et al Nucleic Acids Res. 2013 Oct 10.
    Inserts
    5' iRFP arm
    eGFP with I-Sce I TS
    +3 mCherry
    3' iRFP arm
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    embedded I-Sce I TS from 163-185bp, truncated 25 …
    Promoter
    SFFV
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    45576-plasmid-map-sequence-id-67850

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Showing: 2321 - 2340 of 34175 results