30,577 results

1. pGEM-Oct4

   Plasmid

   #16352

   Depositor

   James Thomson

   Article

   Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

   Insert

   Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (POU5F1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a T7pCONS TIR-1 sfGFP

   Plasmid

   #154463

   Purpose

   pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Use

   Tags

   His-thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-28a (+)-hTFIIB

   Plasmid

   #171084

   Purpose

   Expresses human TFIIB in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TFIIB

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Insert

   TF2B (GTF2B Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSB207

   Plasmid

   #164122

   Purpose

   J23101 driving PP7 repeats

   Depositor

   Christopher Voigt

   Article

   Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

   Insert

   YFP-PP7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. IL-2 promoter luciferase

   Plasmid

   #12194

   Depositor

   Anjana Rao

   Article

   Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

   Insert

   IL-2 promoter (Il2 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. keratin 8 (krt8)-H2B-RFP

   Plasmid

   #105955

   Purpose

   transgenesis

   Depositor

   Caren Norden

   Article

   Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

   Insert

   krt8 (KRT8 Human)
   

   Use

   Tags

   RFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-GST-AGO2

   Plasmid

   #24317

   Depositor

   Carl Novina

   Article

   Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

   Insert

   human AGO2 (AGO2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-28c(+)-YTHDF2

   Plasmid

   #102275

   Purpose

   For bacterial expression of His-tagged human YTHDF2

   Depositor

   Samie Jaffrey

   Article

   Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

   Insert

   YTHDF2 (YTHDF2 Human)
   

   Use

   Tags

   6X HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. HotPETase_pET21b

   Plasmid

   #215407

   Purpose

   Expression construct for HotPETase gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   HotPETase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCWori cytochrome P450-BM3h 9D7

    Plasmid

    #61308

    Purpose

    Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

    Depositor

    Alan Jasanoff

    Article

    Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

    Insert

    Cytochrome P450-BM3 heme domain 9D7
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    T268A, I263A, T438V, A328G

    Promoter

    Tac

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSB208

    Plasmid

    #164123

    Purpose

    Ptac driving PP7 repeats

    Depositor

    Christopher Voigt

    Article

    Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

    Insert

    YFP-PP7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZP06B

    Plasmid

    #232321

    Purpose

    Make sacB a counterselection marker to replace galK

    Depositor

    Chenggang Wu

    Insert

    PrpsJ-sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTRK669

    Plasmid

    #71313

    Purpose

    Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

    Depositor

    Rodolphe Barrangou, Todd Klaenhammer

    Article

    Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

    Type

    Empty backbone

    Use

    E. coli-gram positive bacteria shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJYS1Ptac

    Plasmid

    #85545

    Purpose

    Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    recT
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac and unknown

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRE140

    Plasmid

    #118492

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRMCD28

    Plasmid

    #167556

    Purpose

    Plasmid pWSK29 encoding E. coli PhoA' for making reporter fusion proteins for topology mapping

    Depositor

    Renato Morona

    Article

    Daniels et al Mol Microbiol. 1998 Jun;28(6):1211-22. d

    Type

    Empty backbone

    Use

    Tags

    PhoA'

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST-TACC3-His6

    Plasmid

    #69106

    Purpose

    For bacterial expression of recombinant human TACC3 tagged with GST and His.

    Depositor

    Stephen Royle

    Article

    Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

    Insert

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    

    Use

    Tags

    GST and His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEM-Sox2

    Plasmid

    #16353

    Depositor

    James Thomson

    Article

    Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

    Insert

    Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETM6-G6-vioABE-4A6-vioC

    Plasmid

    #73439

    Purpose

    Deoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    PG6-vioABE-P4A6-vioC
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PG6 and P4A6 (orthogonal T7-lac variants)

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDule-pCNF

    Plasmid

    #85494

    Purpose

    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

    Insert

    para-cyanophenylalanine Mj synthetase
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    lpp (constitutive)

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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