29,712 results

1. pSCrhaB2

   Plasmid

   #113634

   Purpose

   An expression vector containing a rhamnose-inducible promoter to regulate gene expression in Burkholderia cenocepacia

   Depositor

   Miguel Valvano

   Article

   Cardona et al Plasmid. 2005 Nov;54(3):219-28. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaB

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBHSTC104

   Plasmid

   #124356

   Purpose

   Plasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin

   Depositor

   Hideo Iwai

   Article

   Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

   Insert

   gp41-1 N-intein and C-intein (bicistronic)
   

   Use

   Tags

   CcdA-C (SFAD)

   Expression

   Bacterial

   Mutation

   Promoter

   STC

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMV306hsp+FFluc

   Plasmid

   #26156

   Depositor

   Siouxsie Wiles, Brian Robertson

   Article

   Andreu et al PLoS One. 2010 May 24;5(5):e10777.

   Insert

   Firefly luciferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Firefly luciferase gene codon optimised for mycob…

   Promoter

   Available since

   Feb. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRsetB-His7tag-Peredox-mCherry

   Plasmid

   #32382

   Depositor

   Gary Yellen

   Article

   Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

   Insert

   His7-Peredox-mCherry
   

   Use

   Tags

   His7tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. hCD12

   Plasmid

   #225541

   Purpose

   Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.

   Depositor

   David Christianson

   Article

   Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

   Insert

   Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
   

   Use

   Tags

   His6-MBP tag with cleavable TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SNX10-PX (1-201)

   Plasmid

   #119091

   Purpose

   Bacterial expression of human phox homology (PX) domain, SNX10-PX (1-201)

   Depositor

   Brett Collins

   Article

   Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

   Insert

   SNX10-PX (1-201) (SNX10 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD/myc-his A Rv0712 (FGE)

   Plasmid

   #16132

   Purpose

   Bacterial expression of Mycobacterium tuberculosis FGE

   Depositor

   Carolyn Bertozzi

   Article

   Carrico et al Nat Chem Biol. 2007 Jun . 3(6):321-2.

   Insert

   Formylglycine-generating enzyme
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDB016

   Plasmid

   #99220

   Purpose

   Accessory plasmid for aaRS PACE; negative selection

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   PT7 [SD8] gIII-neg
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PT7

   Available since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-28a(+)-GDH

   Plasmid

   #108954

   Purpose

   heterologous expression of GDH of B. megaterium in E. coli

   Depositor

   Frank Schulz

   Article

   Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

   Insert

   Glucose dehydrogenase IV (GDH IV)
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. USP2

    Plasmid

    #36894

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    USP2 (USP2 Human)
    

    Use

    Tags

    His tag with thrombin cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pNitET-SacB-kan

    Plasmid

    #107692

    Purpose

    Expression of the phage Che9c RecET system for Recombineering in mycobacteria

    Depositor

    Kenan Murphy

    Article

    Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

    Inserts

    Che9 phage RecET
    PNit2-NitR
    SacRB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pComb3XLambda

    Plasmid

    #63892

    Purpose

    Backbone pComb3X containing PCR template for human antibody lambda light chain constant region

    Depositor

    Carlos Barbas

    Article

    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

    Insert

    human antibody lambda light chain (IGLL5 Human)
    

    Use

    Phage display

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKM493

    Plasmid

    #140191

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    TEV-Flag-Gly4-eGFP
    

    Use

    Tags

    Flag + eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    PGroEL

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. GST HP1 gamma

    Plasmid

    #24076

    Depositor

    Naoko Tanese

    Article

    Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

    Insert

    HP1 gamma (CBX3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pUC-GFP-HS

    Plasmid

    #133305

    Purpose

    "Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisation

    Depositor

    Chris Barnes

    Article

    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

    Insert

    hok/sok toxin-antitoxin cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. WDR5 (2H9N)

    Plasmid

    #25229

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WDR5_01 (WDR5 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

17. CJ8421_04975 Rptr2 recording plasmid

    Plasmid

    #194211

    Purpose

    CJ8421_04975 Rptr2 targeted DNA sequence

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    CJ8421_04975 Rptr2 DNA target
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPBAD-yhjH

    Plasmid

    #111613

    Purpose

    Arabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjH

    Depositor

    Tim Tolker-Nielsen

    Article

    Christensen et al Infect Immun. 2013 Aug;81(8):2705-13. do

    Insert

    yhjH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A52V

    Promoter

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Nkx2.1

    Plasmid

    #15540

    Depositor

    John Rubenstein

    Article

    Shimamura et al Development. 1995 Dec . 121(12):3923-33.

    Insert

    Nkx2.1 (Nkx2-1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFloat.cspA_v1

    Plasmid

    #133071

    Purpose

    Entry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-His6 and His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    cspA

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge