30,738 results

1. pShHELIX_sgRNA_entry (CJT32)

   Plasmid

   #181782

   Purpose

   Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQTEV-PSMD11

   Plasmid

   #31333

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 (PSMD11 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

   Plasmid

   #213024

   Purpose

   Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

   Depositor

   Martin S. Taylor, Oliver Weichenrieder

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (238-1061)
   

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Promoter

   T7 / Lac

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLG001 (empty vector)

   Plasmid

   #157879

   Purpose

   Empty vector backbone for defense systems

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   None
   

   Expression

   Bacterial

   Available Since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28_Cas9d_Plasmid

   Plasmid

   #239445

   Purpose

   Expression plasmid codon-optimized for E. coli

   Depositor

   David Taylor

   Article

   Ocampo et al Nat Commun. 2025 Jan 7;16(1):457. doi: 1

   Insert

   Cas9d
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28_ancCas9d_Plasmid

   Plasmid

   #239444

   Purpose

   Expression plasmid codon-optimized for E. coli

   Depositor

   David Taylor

   Article

   Ocampo et al Nat Commun. 2025 Jan 7;16(1):457. doi: 1

   Insert

   ancCas9d
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. RNAPII_CTD27-52

   Plasmid

   #98678

   Purpose

   Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

   Depositor

   Nicolas Fawzi

   Article

   Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

   Insert

   RNA polymerase II C-terminal domain (POLR2A Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a-His-IleRS

   Plasmid

   #124112

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Isoleucyl-tRNA ligase (ileS Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTHSSe_59

   Plasmid

   #109253

   Purpose

   TALEsp1 stabilized promoter driving GFP

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Expression

   Bacterial

   Promoter

   TALEsp1 stabilized promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSUMO3

    Plasmid

    #52260

    Purpose

    Bacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-3 (SUMO3 Human)
    UBE2l (UBE2I Human)
    SAE-2 (UBA2 Human)
    SAE-1 (SAE1 Human)
    

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMV306hsp+Lux

    Plasmid

    #26159

    Depositor

    Brian Robertson, Siouxsie Wiles

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    Bacterial luciferase operon
    

    Expression

    Bacterial

    Mutation

    It contains a gram-positive enhanced translation …

    Available Since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. SeAvs3

    Plasmid

    #188791

    Purpose

    Avs3 from Salmonella enterica NCTC13175

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    SeAvs3
    

    Expression

    Bacterial

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTPH405c

    Plasmid

    #185726

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    gIII(N)-NpuN-NpuC-gIII(C) single PAM linker
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLPT119

    Plasmid

    #85526

    Purpose

    Repressilator without degradation tags, with integrated reporter

    Depositor

    Johan Paulsson

    Article

    Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

    Inserts

    mCfp
    mVenus
    tetR
    lacI
    cI
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. ELONGIN BC (XX01TCEB1A-c001)

    Plasmid

    #110274

    Purpose

    Elongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin C

    Depositor

    Nicola Burgess-Brown

    Insert

    Elongin BC (ELOC, ELOB Human)
    

    Expression

    Bacterial

    Mutation

    Elongin B is full length. Elongin C contains a.a…

    Promoter

    T7

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQE30-His-IF2

    Plasmid

    #124124

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Initiation factor 2 (infB Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a-LeuRS-His

    Plasmid

    #124113

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Leucyl-tRNA ligase (leuS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a-TrpRS-His

    Plasmid

    #124120

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Tryptophanyl-tRNA ligase (trpS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBlu-CGT-1

    Plasmid

    #170158

    Purpose

    Expresses bacterial Cyclomaltodextrin Glucanotransferase from Paenibacillus sp. c36 in E.coli

    Depositor

    Sarah MacDonald

    Insert

    Cyclomaltodextrin Glucanotransferase
    

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMBP-HRV3C-AcrIF9

    Plasmid

    #141442

    Purpose

    Expression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.

    Depositor

    Blake Wiedenheft

    Article

    Hirschi et al Nat Commun. 2020 Jun 1;11(1):2730. doi:

    Insert

    AcrIF9
    

    Tags

    HRV3C cleavage site and Maltose Binding Protein

    Expression

    Bacterial

    Promoter

    ptac

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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