32,387 results
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Plasmid#166502PurposeExpression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertalkS
UseSynthetic BiologyExpressionBacterialMutationL401GPromoterPalkSAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM6-G6-vioABE-4A6-vioC-3A2-vioD
Plasmid#73440PurposeViolacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.DepositorInsertPG6-vioABE-P4A6-vioC-P3A2-vioD
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPG6, P4A6, and P3A2 (orthogonal T7-lac variants)Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTKLP-tetA
Plasmid#71325PurposetetA landing pad template plasmidDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1 PDZD8-WT
Plasmid#227849PurposeTo express in E. coli PDZD8-WTDepositorAvailable SinceJuly 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
p005-RFP-strong
Plasmid#108317PurposeSynthetic RFP based on Corynactis spp under strong synthetic constitutive promoter pFAB4005; kanamycin resistant.DepositorInsertRFP under controlf of strong synthetic constitutive promoter pFAB4005
ExpressionBacterialPromoterpFAB4005Available SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC-SP6
Plasmid#102418PurposeAc transposase with SP6 promoter for in vitro transcriptionDepositorInsertAc transposase
ExpressionBacterialPromoterSP6Available SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
RepX_pET28a(+)
Plasmid#172199PurposeExpresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.DepositorInsertRepX superhelicase
Tags6xHis tagExpressionBacterialMutationC18L, C43S, C167V, S400C, C612AAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCN38-yfp
Plasmid#227637PurposeNegative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.DepositorInsertyfp
ExpressionBacterialAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX_hDDX5_FL_wt
Plasmid#88871Purposeinducible expression of GST-DDX5 in bacteriaDepositorAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pScaf-1512.1
Plasmid#111402PurposepScaf phagemid with insert for generating DNA origami scaffold 1512 bases in lengthDepositorInsertpScaf-1512
ExpressionBacterialAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL0284 (pQCascade_entry)
Plasmid#130635PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJ251-GERC
Plasmid#47441PurposeExpresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.DepositorInsertGene Expression Reporter Construct
ExpressionBacterialAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET22b-msfGFP-NPM1
Plasmid#237670PurposeFor expression of msfGFP-NPM1 in EcoliDepositorAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSB235
Plasmid#164130PurposePhlF-RFP expression plasmidDepositorInsertPhlF-RFP
ExpressionBacterialPromoterPtetAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMyNT-kan
Plasmid#84693PurposeProtein expression in mycobacteriaDepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterAcetamidaseAvailable SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSM019
Plasmid#128291Purposeasymmetric plasmid partitioning E. coli - regulatory plasmidDepositorInsertPara - lp-sfyfp-parB
ExpressionBacterialAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
p6XHis_NLS-SaCas9
Plasmid#101086PurposeExpress SaCas9 in bacteria with a 6xHis tag for purificationDepositorInsertCRISPR-associated protein Cas9/Csn1
UseCRISPRTagsTrxA-6xHis-STagExpressionBacterialPromoterT7lacAvailable SinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LuxI
Plasmid#193626PurposeConstitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.DepositorInsertLuxI
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
ED_p_126
Plasmid#246073PurposeBacterial expression of ECOR31 2TMbeta residues 72-200 with N-terminal His-SUMO tag and co-expression of ECOR31 mCpolDepositorInsert2TMbeta
ExpressionBacterialAvailable SinceOct. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQF-lacZ
Plasmid#48094PurposeCumate-inducible expression of lacZDepositorInsertE. coli lacZ
ExpressionBacterialPromoterPQ5Available SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only