30,602 results

1. nifF in pET21b

   Plasmid

   #124520

   Purpose

   Azotobacter vinelandii flavodoxin II of Nitrogenase

   Depositor

   Douglas Rees

   Article

   Segal et al Protein Sci. 2017 Oct;26(10):1984-1993.

   Insert

   Flavodoxin II (nifF Azotobacter vinelandii)
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   T7 / lac

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKD236-4b

   Plasmid

   #90956

   Purpose

   Expresses ThsS constitutively

   Depositor

   Jeffrey Tabor

   Article

   Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

   Insert

   ThsS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Bba_J23104

   Available Since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-Ki67-mCherry

   Plasmid

   #183748

   Purpose

   Expresses EGFP-Ki67-mCherry

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 (MKI67 Human)
   

   Use

   Tags

   Ki-67 with N-terminal EGFP tag and Ki-67 with C-…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSMART-SOS-GFPuv

   Plasmid

   #102283

   Purpose

   Fluorescent sensor of E. coli SOS response

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   cda-GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   cda SOS promoter

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJLG038

   Plasmid

   #192981

   Purpose

   BHR plasmid (pBBR1 ori) expressing T7 polymerase under control of the lac promoter

   Depositor

   Clay Fuqua

   Article

   Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

   Insert

   T7 polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-6His-MBP-MpAgo

   Plasmid

   #79249

   Purpose

   This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression

   Depositor

   Jennifer Doudna

   Article

   Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p

   Insert

   Marinitoga piezophila Argonaute
   

   Use

   CRISPR

   Tags

   6XHis-tag + MBP + PreScission Cleavage Site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHybrid-Electra1

   Plasmid

   #179474

   Purpose

   Expresses Electra1 in bacterial cells and Electra1 and mScarlet in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Inserts

   Electra1
   mScarlet-I
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET24a-VHH-std

   Plasmid

   #109417

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody. VHH-std also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. His-GST-TEV-BRD4(42-459) BD1-BD2

   Plasmid

   #233887

   Purpose

   pGex for protein overexpression of human BRD4

   Depositor

   Alessio Ciulli

   Article

   Hsia et al Nature. 2024 Mar;627(8002):204-211. doi:

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   His-GST-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. EHMT2

    Plasmid

    #25503

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EHMT2 (EHMT2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pAWP78-PVCpnf_pvc13-antiMouseMHCIINb

    Plasmid

    #198292

    Purpose

    Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-antiMouseMHCIINb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-5X-TRIM28

    Plasmid

    #45570

    Depositor

    Fanxiu Zhu

    Article

    Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available Since

    May 29, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pPath-Violacein-SGE

    Plasmid

    #200003

    Purpose

    Violacein pathway. SGE

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Pathways
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3993 pET ubcH7

    Plasmid

    #8654

    Depositor

    Peter Howley

    Article

    Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

    Insert

    UbcH7 (UBE2L3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pZS2-123

    Plasmid

    #26598

    Depositor

    Michael Elowitz

    Article

    Cox RS et al J Biol Eng. 2010 Jul 21;4:10. doi: 10.11

    Insert

    Synthetic
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available Since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pColE1-ISceI

    Plasmid

    #179578

    Purpose

    Expresses I-SceI from an arabinose promoter

    Depositor

    Kevin Solomon

    Article

    Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

    Insert

    I-SceI (I-SceI Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDNA2.0 6H-FLAG-TEV-KRAS G12C

    Plasmid

    #111848

    Purpose

    Expresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.

    Depositor

    Kenneth Westover

    Article

    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

    Insert

    KRAS G12C (KRAS Human)
    

    Use

    Tags

    6xHis-tag, FLAG-tag, and TEV protease cleavage se…

    Expression

    Bacterial

    Mutation

    Changed Glycine 12 to Cysteine

    Promoter

    T5

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-53-RtcB

    Plasmid

    #51282

    Purpose

    E. coli expression vector for E. coli RtcB RNA ligase

    Depositor

    Jay Hesselberth

    Article

    Peach et al Nucleic Acids Res. 2015 May 22. pii: gkv

    Insert

    E. coli RtcB
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNA Polymerase

    Available Since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTP261

    Plasmid

    #104166

    Purpose

    Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
    

    Use

    Tags

    14x Histidine tag and NEDD8 from Brachypodium dis…

    Expression

    Bacterial

    Mutation

    1x Cysteine located at bps 493-495 in full sequen…

    Promoter

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. IL-4 promoter luciferase

    Plasmid

    #12195

    Depositor

    Anjana Rao

    Article

    Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

    Insert

    IL-4 promoter (Il4 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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