34,178 results
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Plasmid#177277PurposeEncodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.DepositorInsertRFP, CauNEO
TagsENO1-RFPExpressionBacterialMutationAll CUG Leucine codons were replaced with the syn…PromoterAgTEF2Available sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-NTD
Plasmid#171235PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain (NTD)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert2xHA-tagged human androgen receptor trancate: N-terminal-domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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p70a-T7RNAP
Plasmid#105683PurposeExpresses T7 polymerase in cell-free TXTL.DepositorInsertT7 polymerase
ExpressionBacterialPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE-60NA-proinsulin-msGFP2
Plasmid#160466PurposeEncodes a fusion of human proinsulin and the monomeric superfolder GFP2DepositorInsertproinsulin-msGFP2
ExpressionBacterialPromoterT5Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pM2s2TsR
Plasmid#137923PurposeE. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…ExpressionBacterialPromoterJ23101Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG007 (Ec86 retron)
Plasmid#157885PurposeExpresses the Ec86 retron defense systemDepositorInsertEc86 retron
ExpressionBacterialAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
bNS2B47NS3
Plasmid#155320Purposeco-expression of DENV4 47 peptides from NS2B cofactor and NS3 full lengthDepositorInsertCo expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationWTPromoterT7Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
GEF-H1 FLARE212.pa
Plasmid#134930PurposeGEF-H1 biosensor that does not have GEF activity.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
TagsNone.ExpressionBacterial and MammalianPromoterCMV and T7Available sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
T7 promoter + terminators reporter plasmid
Plasmid#156456PurposeA mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.DepositorInsertsNeoR/KanR
TcR
ExpressionBacterialMutationATG start codon mutated to ACGPromoterEm7 and tetAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF
Plasmid#149446PurposeExpression of WT MIF in E. coli for protein productionDepositorAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-His-SCov2-nsp8
Plasmid#154758PurposeExpression of SARS-CoV-2 nsp8DepositorInsertSARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionBacterialPromoterT7Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGBW-m4046920
Plasmid#145717PurposeBacterial Expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVDM1001
Plasmid#134660PurposeCRISPR delivery and repair template delivery vectorDepositorInsertCRISPR guide RNA array
ExpressionBacterialAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH1105
Plasmid#131864PurposeProduces holo-PhyB-AviTag in E. coliDepositorInsertsPhyB(1-651)-AviTag-His6
HO1
PcyA
ExpressionBacterialPromoterT7 promoter-1 and T7 promoter-2Available sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC1749
Plasmid#127983PurposeAMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTOX1
Plasmid#127446PurposeEncodes YhaV toxin. CAM-RDepositorInsertyhaV toxin
ExpressionBacterialPromoterpRhaAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAR17_StrepI_hFASh_H8_pET22b
Plasmid#122846PurposeExpresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInserthuman FASN/ human fatty acid synthase (FASN Human)
TagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAR357_SFP_pCDF-1b
Plasmid#122851PurposeCo-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.DepositorInsertSfp
ExpressionBacterialPromoterT7 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1
Plasmid#122083PurposeExpress M.EcoGII fused to LaminB1 with detabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII-V5-LaminB1
UseRetroviralTagsV5 tag on Nter of LaminB1ExpressionBacterial and MammalianAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by