33,441 results

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pC0075 TtCsm6 His6-TwinStrep-SUMO-BsaI

Plasmid

#115270

Purpose

Expresses TtCsm6 from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

TtCsm6

Tags

SUMO

Expression

Bacterial

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a-SpyCatcher-mi3

Plasmid

#112255

Purpose

Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

Depositor

Mark Howarth

Publication

Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

Insert

SpyCatcher-mi3

Tags

C-tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJL1-dTomato

Plasmid

#102631

Purpose

In vitro expression of tdTomato from the T7 promoter

Depositor

Michael Jewett

Publication

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

tdTomato

Expression

Bacterial

Promoter

T7

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pMP2463

Plasmid

#107774

Purpose

Expression of EGFP

Depositor

Herman Spaink

Publication

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-EGFP in pBBR1-mcs5

Tags

pLacZ-EGFP

Expression

Bacterial

Promoter

pLacZ

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDNA2.0 6H-TEV-KRAS G13D

Plasmid

#111851

Purpose

Expresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Depositor

Kenneth Westover

Publication

Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

Insert

KRAS G13D (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Mutation

Changed Glycine 13 to Aspartic Acid

Promoter

T5

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA_mTurquoise2

Plasmid

#108237

Purpose

Broad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

Depositor

Rosemarie Wilton

Publication

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

PsbA promoter - mTurquoise2

Expression

Bacterial

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn G51D

Plasmid

#105747

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Publication

Fares et al Hum Mol Genet. 2014 Sep 1;23(17):4491-50

Insert

alpha-synuclein G51D (SNCA Human)

Expression

Bacterial

Mutation

G51D

Promoter

T7

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mScarlet-I

Plasmid

#104028

Purpose

Plasmid encoding 2ndVal mScarlet-I

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mScarlet-I

Use

Low copy

Expression

Bacterial

Mutation

mScarlet-I with valine inserted in 2nd position

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMyCA

Plasmid

#84268

Purpose

A versatile vector for recombinant mycobacterial protein production

Depositor

Young-Hwa Song

Publication

Magana Vergara et al Protein Sci. 2017 Aug 31. doi: 10.1002/p

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

Aceteamidase

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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Profiline

Plasmid

#89925

Purpose

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin

Depositor

Antoine Jégou, Guillaume Romet-Lemonne

Publication

Jegou et al PLoS Biol. 2011 Sep;9(9):e1001161. doi:

Insert

profiline-1 (PFN1 Human)

Tags

None

Expression

Bacterial

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pBad-HisB-GRvT

Plasmid

#87363

Purpose

Expresses GRvT, a tandem heterodimer green and red fluorescent protein

Depositor

Robert Campbell

Publication

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

GRvT

Tags

His-Tag

Expression

Bacterial

Promoter

AraBad

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWUKI 1+2

Plasmid

#79879

Purpose

Encodes Cas1+2 and a CRISPR array

Depositor

George Church

Publication

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1
Cas2
CRISPR Array

Expression

Bacterial

Promoter

T7

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pECGMC3

Plasmid

#75441

Purpose

Plasmid for mf-lon mediated mCherry degradation in Lactococcus lactis. Contains mcherry-pdt3

Depositor

James Collins

Publication

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

mf-lon

Expression

Bacterial

Promoter

nisA promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUCP30T-mKo1

Plasmid

#78477

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Publication

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

mKO1

Expression

Bacterial

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pET21-Drp1

Plasmid

#72927

Purpose

Bacterial expression of mouse Drp1 isoform b (699amino acids) with C-terminal His-tag and PreScission protease cleavage site

Depositor

David Chan

Publication

Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

Insert

Drp1 (Dnm1l Mouse)

Tags

6xHis-tag and PreScission Protease site

Expression

Bacterial

Promoter

T7

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGM190

Plasmid

#69614

Purpose

inducible gene expression in Streptomyces

Depositor

Günther Muth

Publication

Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

Inserts

aphII
tsr
rep
sso
TipA promoter
dso

Use

Streptomyces- e. coli shuttle vector

Expression

Bacterial

Available since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pY003 (pFnCpf1_delta Cas)

Plasmid

#69974

Purpose

Expresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.

Depositor

Feng Zhang

Publication

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus without Cas genes

Use

CRISPR

Expression

Bacterial

Mutation

Cas genes are deleted from locus

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRAV23

Plasmid

#67715

Purpose

Parental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag for native purification

Depositor

Robert Batey, Jeffrey Kieft

Publication

Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

Insert

Transcription cassette for 3'-tagged RNAs

Expression

Bacterial

Promoter

T7 RNA polymerase

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only
pBT1-proD-mCherry

Plasmid

#65823

Purpose

Const. mCherry expression , proD promoter, E. coli Expression

Depositor

Michael Lynch

Insert

mCherry

Expression

Bacterial

Promoter

proD E . coli Constitutive Promoter

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCRISPReporter-mCherry

Plasmid

#65008

Purpose

E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.

Depositor

Mattheos Koffas

Publication

Cress et al ACS Synth Biol. 2015 Mar 30.

Insert

mCherry

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

User-defined

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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