30,738 results

1. pQE60-EFTs-His

   Plasmid

   #124128

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Elongation factor Ts (tsf Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRelA*

   Plasmid

   #175590

   Purpose

   Tet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.

   Depositor

   Greg Bokinsky

   Article

   Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

   Insert

   Catalytic domain of E.coli relA gene.
   

   Tags

   Fused with a flexible GS linker to mVenus

   Expression

   Bacterial

   Mutation

   Only the catalytic domain of the enzyme is used (…

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZ8-T_dCas9

   Plasmid

   #74062

   Purpose

   pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   dcas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   ptac

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET9d-hnRNP-A1

   Plasmid

   #23026

   Depositor

   Douglas Black

   Insert

   hnRNPA1 (HNRNPA1 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTHSSe_49

   Plasmid

   #109247

   Purpose

   pSC101 var7 BbsI cloning vector

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Expression

   Bacterial

   Promoter

   PJ23105

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-24a-ELP[V-30]

   Plasmid

   #67014

   Purpose

   Expresses ELP[V-30] in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   ELP[V-30]
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. 37 pCOLI_K_STREP

   Plasmid

   #160263

   Purpose

   N-term 2xStrepII 3C cleavable Kan resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   2xStrepII

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACyR1

   Plasmid

   #172565

   Purpose

   Inducible gene expression, pACYC origin, CymRAM repressor, PCymRC promoter, mRFP, Chloramphenicol Resistance Marker.

   Depositor

   Christopher Reisch

   Insert

   CymRAM-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PCymRC

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCWori cytochrome P450-BM3h 9D7

   Plasmid

   #61308

   Purpose

   Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

   Depositor

   Alan Jasanoff

   Article

   Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

   Insert

   Cytochrome P450-BM3 heme domain 9D7
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   T268A, I263A, T438V, A328G

   Promoter

   Tac

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Brg1 Del2 pET28a

    Plasmid

    #122116

    Purpose

    Expression Brg1 amino acids 1-837 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Tags

    6XHis and His-T7

    Expression

    Bacterial

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMycVec2

    Plasmid

    #32361

    Depositor

    Michael Niederweis

    Article

    Kaps et al Gene. 2001 Oct 31;278(1-2):115-24.

    Insert

    pJAZ38 ori
    

    Expression

    Bacterial

    Available Since

    Nov. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMDH

    Plasmid

    #133129

    Purpose

    A recombinant plasmid expressing mdh (malate dehydrogenase) gene

    Depositor

    Gregg Beckham

    Article

    Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    malate dehydrogenase gene
    

    Expression

    Bacterial

    Promoter

    malate dehydrogenase promoter

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. ecALDH

    Plasmid

    #67921

    Purpose

    Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Insert

    Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG79-attL1-pegRNA-attL2

    Plasmid

    #213050

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    35S-CmYCLV-AtU6

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQLink-GST-4xCTD

    Plasmid

    #138471

    Purpose

    Expresses GST-tagged 4xCTD heptapeptide repeat in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    Rpb1 (POLR2A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    4x CTD repeat

    Promoter

    T5

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-HxB.L

    Plasmid

    #65414

    Purpose

    contains complete cDNA for TNC large splice variant flanked by NotI-KpnI

    Depositor

    Harold Erickson

    Article

    Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

    Insert

    TNC large splice variant (TNC Human)
    

    Expression

    Bacterial

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPSU50

    Plasmid

    #173832

    Purpose

    Expresses 50 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABMBP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-GST-AGO2

    Plasmid

    #24317

    Depositor

    Carl Novina

    Article

    Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

    Insert

    human AGO2 (AGO2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. GST-PR-LBD

    Plasmid

    #89117

    Purpose

    bacterial expression of human progesterone receptor ligand binding domain amino acid residues 637-933 with GST tag in pGEX-3X-1

    Depositor

    Elizabeth Wilson

    Insert

    human progesterone receptor ligand binding domain (PGR Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains Ligand Binding Domain (637-933)

    Promoter

    tac

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pY001 (pFnCpf1_full)

    Plasmid

    #69973

    Purpose

    Expresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array.

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 locus
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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