30,260 results

1. pAD43-daf-16 RNAi

   Plasmid

   #34833

   Depositor

   Cynthia Kenyon

   Article

   Dillin et al Science. 2002 Oct 25;298(5594):830-4.

   Insert

   daf-16 (daf-16 Nematode)
   

   Use

   RNAi

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 13, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pET32a-HD16Q

   Plasmid

   #11487

   Depositor

   Pamela Bjorkman

   Article

   Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

   Insert

   huntingtin, exon 1, 16 glutamines (HTT Human)
   

   Use

   Tags

   His, His (out of frame), and Trx

   Expression

   Bacterial

   Mutation

   huntingtin exon 1 with 16 glutamines, vector m…

   Promoter

   Available since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

   Plasmid

   #110201

   Purpose

   Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   LexPR transactivator - 2A - Cerulean
   

   Use

   Tags

   Cerulean

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET22b - PA

   Plasmid

   #84863

   Purpose

   bacterial expression of Bacillus anthracis protective antigen

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Insert

   PA
   

   Use

   Tags

   pelB signal sequence

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNBU2_erm_P1T_DP-GH023 - NanoLuc

   Plasmid

   #117727

   Purpose

   Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023 upstream of the NanoLuc reporter

   Depositor

   Andrew Goodman

   Article

   Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

   Insert

   NanoLuc
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Parp14-c012

   Plasmid

   #173099

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP14 (PARP14 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   W1003-A1190

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEQ111m

   Plasmid

   #40190

   Depositor

   Paul Modrich

   Article

   Wright et al J Biol Chem. 1989 Jul 15;264(20):11816-2

   Insert

   EcoRI endonuclease & EcoRI methylase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Glutamic Acid 111 to Glutamine

   Promoter

   Lambda PL

   Available since

   Nov. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)

   Plasmid

   #218513

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferase

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   deletion of the Plac_mrfp cassette

   Promoter

   PgyrA_ermBL-ermB'

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQE9-His-p97(wt)

   Plasmid

   #14666

   Depositor

   Graham Warren

   Article

   Meyer et al EMBO J. 2000 May 15. 19(10):2181-92.

   Insert

   p97 (Vcp Mouse)
   

   Use

   Tags

   RGS6xHis

   Expression

   Bacterial

   Mutation

   See depositor comments below.

   Promoter

   Available since

   April 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-AviTag-DogTag-MBP

    Plasmid

    #171773

    Purpose

    Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    AviTag-DogTag-MBP
    

    Use

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Peptide tag added to N-terminus of MBP via a GSGE…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCdrA-gfpC

    Plasmid

    #111614

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Parp14-c010

    Plasmid

    #173100

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP14 (PARP14 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    F1208-G1388

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNR220

    Plasmid

    #79541

    Purpose

    Input plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Inserts

    TP901
    BxbI
    A118
    

    Use

    Tags

    His tag and LAA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-GST-His6-2_HBD

    Plasmid

    #171870

    Purpose

    Expresses tandem repeats of HBD domain of RNase H1 for better DNA-RNA Hybrid recognition specificity in bacterial cells

    Depositor

    Kaiwei Liang

    Article

    Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

    Insert

    Tandem repeats Hybrid binding domain of Rnase H1
    

    Use

    Tags

    GST- and His10-tag

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SIRT3L-12

    Plasmid

    #13736

    Depositor

    John Denu

    Article

    Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

    Insert

    SIRT3 (SIRT3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115220

    Purpose

    Expresses Pin3Cas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    Pin3Cas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST14-Cys-DogCatcher

    Plasmid

    #171929

    Purpose

    Express Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    Cys-DogCatcher
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115210

    Purpose

    Expresses AspCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    AspCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUCP20T-morange

    Plasmid

    #78468

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    mOrange
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET15bpATnp

    Plasmid

    #121137

    Purpose

    This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

    Depositor

    Keji Zhao

    Article

    Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

    Insert

    Protein A-Tnp
    

    Use

    Tags

    his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge