30,743 results

1. PAP-delta10-cHIS

   Plasmid

   #21716

   Depositor

   Andrew Bohm

   Article

   Balbo et al Biochemistry. 2005 May 31. 44(21):7777-8

   Insert

   polyA polymerase (PAP1 Budding Yeast)
   

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   30 amino acids off the c terminus

   Available Since

   Aug. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

2. Brg1 Del3 pET28a

   Plasmid

   #122117

   Purpose

   Expression Brg1 amino acids 836-1446 in bacteria

   Depositor

   Paul Herring

   Article

   Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

   Insert

   Brg1 (SMARCA4 Human)
   

   Tags

   His-T7

   Expression

   Bacterial

   Available Since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-4T1 human PP2A Aα full length

   Plasmid

   #132634

   Purpose

   expresses human PP2A Aα full length in E. coli

   Depositor

   Wenqing Xu

   Article

   Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

   Insert

   PP2A Aα (PPP2R1A Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK792

   Plasmid

   #8830

   Depositor

   David Waugh

   Article

   Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

   Insert

   TEV protease, S219P mutant
   

   Tags

   His, MBP (cleaved), and polyarginine

   Expression

   Bacterial

   Mutation

   S219P mutation

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b-His-RF2

   Plasmid

   #124130

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Release factor 2 (prfB Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEvol-MmKlacRS1

   Plasmid

   #232157

   Purpose

   Site-specific incorporation of L-lactyl-lysine into target proteins in E. coli cells.

   Depositor

   Nanxi Wang

   Article

   Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

   Insert

   KlacRS1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLuc-N-HiBiT

   Plasmid

   #207117

   Purpose

   Bacterially expressed HiBiT-FLuc for Ni-NTA purification

   Depositor

   James Inglese

   Article

   Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

   Insert

   luciferin 4-monooxygenase
   

   Tags

   HiBiT Tag and His-tag with thrombin site

   Expression

   Bacterial

   Mutation

   Codon optmization for expression in BL21DE3

   Promoter

   T7 Promotor

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTn7xTS_SpyCatcher:mKate2

   Plasmid

   #225188

   Purpose

   Used for inserting SpyCatcher fused to mKate2 at the bacterial Tn7 site

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   SpyCatcher:mKate2
   

   Tags

   SpyCatcher

   Expression

   Bacterial

   Available Since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. VAMP8

   Plasmid

   #186689

   Purpose

   Expresses VAMP8 in E. Coli

   Depositor

   Axel Brunger

   Article

   Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

   Insert

   VAMP8
   

   Tags

   3xFLAG, GST, TEV, and Thrombin

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. 20 pCOLI_A_N-His_C_STREP

    Plasmid

    #160162

    Purpose

    N-term 6xHis 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    2xStrepII and 6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. His-Tev-Nedd4L

    Plasmid

    #186806

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-Nedd4L.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    His-Tev-Nedd4L (NEDD4L Human)
    

    Tags

    His6-Tev

    Expression

    Bacterial

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRIS

    Plasmid

    #120424

    Purpose

    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.

    Depositor

    Tamás Fehér

    Article

    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

    Insert

    CRISPR spacers targeting IS1, IS5, IS3, IS150
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    constitutive

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTSara

    Plasmid

    #60720

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    N Terminal fragment of T7 RNAP split betwen 179-180
    N Terminal fragment of T7 RNAP split betwen 179-180
    

    Expression

    Bacterial

    Mutation

    T7 RNAP split between positions 179-180 and wt T7…

    Promoter

    PBAD

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE2-TetOminiCMV-OSKM

    Plasmid

    #136541

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Sox2, Pou5f1, Myc, Klf4 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-mCherry-hphMX6

    Plasmid

    #105156

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

    Insert

    mCherry-hphMX6
    

    Tags

    mCherry

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET26b_Nb.b201

    Plasmid

    #131404

    Purpose

    E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

    Depositor

    Andrew Kruse

    Article

    McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

    Insert

    Nanobody b201
    

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Terminase_PhiV1

    Plasmid

    #188836

    Purpose

    Large terminase from phage PhiV1

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    Terminase
    

    Expression

    Bacterial

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDB179-ThermoPETase

    Plasmid

    #218698

    Purpose

    Encodes codon optimized ThermoPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    ThermoPETase
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. A24M04 mIgM heavy chain-pFEEKW

    Plasmid

    #234809

    Purpose

    Express membrane form of IgM heavy chain containing the VH region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1

    Depositor

    Susan Pierce

    Article

    Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

    Insert

    A24M04 membrane IgM heavy chain (IGHM Human)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Promoter

    EEK promoter

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX Rin1-SH2

    Plasmid

    #46474

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Rin1 (RIN1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 54-204

    Promoter

    Tac

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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