30,260 results

1. RepX_pET28a(+)

   Plasmid

   #172199

   Purpose

   Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.

   Depositor

   Taekjip Ha

   Article

   Arslan et al Science. 2015 Apr 17;348(6232):344-7. do

   Insert

   RepX superhelicase
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   C18L, C43S, C167V, S400C, C612A

   Promoter

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MBP-FUS_LC_WT

   Plasmid

   #98653

   Purpose

   Expresses MBP-tagged FUS AA 1-163

   Depositor

   Nicolas Fawzi

   Article

   Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCA24N-sso7d-ligase

   Plasmid

   #87745

   Purpose

   IPTG-inducible expression of sso7d-ligase fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Use

   Tags

   6xHis and codon optimized sso7d (Sulfolobus solfa…

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC18/promEco/Lysostaphin

   Plasmid

   #125765

   Purpose

   Production of staphylolytic protein lysostaphin from a constitutive promoter

   Depositor

   Benedykt Wladyka

   Article

   Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

   Insert

   lysostaphin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   sequence corresponding to the mature form protein

   Promoter

   pemIK-Sa1 promoter

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLAM12

   Plasmid

   #26908

   Depositor

   Graham Hatfull

   Article

   van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

   Insert

   Acetamidase Promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

6. HA-mDyn3 pcDNA3

   Plasmid

   #36265

   Depositor

   Pietro De Camilli

   Article

   Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

   Insert

   Dynamin 3 (Dnm3 Mouse)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pRSET his-eGFP

   Plasmid

   #113551

   Purpose

   For bacterial expression and purification of eGFP

   Depositor

   Jeanne Stachowiak

   Article

   Busch et al Nat Commun. 2015 Jul 24;6:7875. doi: 10.

   Insert

   his-eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A206K

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115214

   Purpose

   Expresses PauCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PauCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Pet22b-YAP

   Plasmid

   #166443

   Purpose

   Bacterial expression of 6x His tagged YAP

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   YAP (YAP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST17-UbE2T

    Plasmid

    #15808

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2T (UBE2T Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pBAD-LSSmGFP

    Plasmid

    #186542

    Purpose

    LSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    araB

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. ath5:GFP-CAAX

    Plasmid

    #105958

    Purpose

    transgenesis, EGFP membrane

    Depositor

    Caren Norden

    Article

    Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

    Insert

    CAAX
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCF1148

    Plasmid

    #222330

    Purpose

    Fluorescent reporter plasmid for Veillonella

    Depositor

    J Christopher Fenno

    Article

    Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

    Insert

    bs2 gene with Veillonella parvula SKV38 mdh promoter
    

    Use

    E. coli-veillonella shuttle plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Veillonella parvula SKV38 mdh promoter

    Available since

    Oct. 31, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pET28a-MSP1D1deltaH4-H6

    Plasmid

    #71717

    Purpose

    Membrane scaffold protein (MSP), helix 4 to 6 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTYB1-T4uvsX

    Plasmid

    #228213

    Purpose

    Expression in BL21(DE3) of T4 recombinase

    Depositor

    Stephen Benkovic

    Insert

    uvsX (uvsX Escherichia phage T4)
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTU1-A-SP44-sfGFP

    Plasmid

    #163757

    Purpose

    Expresses Streptomyces codon-optimised sfGFP

    Depositor

    Paul Freemont, Simon Moore

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    SP44

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pG108 - K

    Plasmid

    #178039

    Purpose

    Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers

    Depositor

    Janina Lewis

    Article

    Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

    Insert

    Kanamycin resistance
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ErmAM has been replaced with Kanamycin for select…

    Promoter

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mTFP1-pBAD

    Plasmid

    #54553

    Purpose

    Localization: Protein Expression Vector , Excitation: 462, Emission: 492

    Depositor

    Michael Davidson, Robert Campbell

    Article

    Ai et al Biochem J. 2006 Dec 15. 400(3):531-40.

    Type

    Empty backbone

    Use

    Tags

    6xHis and mTFP1

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21-10XHis-GST-HRV-dL5

    Plasmid

    #73214

    Purpose

    Bacterial expression vector for dL5(E52D) protein with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

    Insert

    EGFR (EGFR Human, Schistosoma japonicum)
    

    Use

    Tags

    10XHis-GST-HRV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAW019-2

    Plasmid

    #85614

    Purpose

    Inducible dCas9 integration vector for Bacillus subtilis

    Depositor

    Perry Chou

    Article

    Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PxylA (B. megaterium)

    Available since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge