29,729 results
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pXTK-BB1 [Backbone_RK2-KanR_PartTypeBB]
Plasmid#229387PurposeXanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_RK2-KanR_PartTypeBB and an sfGFP dropoutDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(pos36)GFP
Plasmid#62937PurposeExpression of 6xHis-(+36)GFP in bacterial cellsDepositorInsert(pos36)GFP
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-11-LECT2-I40
Plasmid#183032PurposeExpression of LECT2 I40 variant protein from T7 promoterDepositorInsertLECT2 (LECT2 Human)
UseTagsExpressionBacterialMutationI40 variantPromoterT7Available sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
PE-Max-pet21A
Plasmid#204471PurposeExpression plasmid for PE protein in bacteria.DepositorInsertPrime editor
UseCRISPRTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Opto-CRAC version 1
Plasmid#101245PurposeGenetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signalingDepositorInsertLOV2 and STIM1 cytosolic fragment
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
elavl3:hGCGR-TEVcs-QF;he1.1:CFP
Plasmid#218852PurposeD. rerio elavl3 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-TangoDepositorInserthGCGR (GCGR Human)
UseTagsQF fusion proteinExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21 gamma-tubulin
Plasmid#101825Purposeproduction of gamma-tubulin in bacteriaDepositorInsertgamma-tubulin 1 (TUBG1 Human)
UseTagsHisExpressionBacterialMutationWhole proteinPromoterT7Available sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
SupD v2
Plasmid#198598PurposePlasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.DepositorInsertSupD
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Hyg)
Plasmid#110084PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221pmCDA1-T7RNAP
Plasmid#167978PurposeExpresses fusion protein pmCDA1-T7RNAP in E. coliDepositorInsertpmCDA1-T7RNAP (T7p07 Synthetic)
UseTagsT7 tag and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A
Plasmid#83485PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).DepositorInsertLbu_C2c2_R472A_H477A_R1048A_H1053A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR472A, H477A, R1048A, H1053APromoterAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II SK--POLQ
Plasmid#64873Purposecloning vector for human POLQDepositorInsertPOLQ (POLQ Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAS528 (TJF059)
Plasmid#65602PurposepETDuet-1 Containing FadD-HisDepositorInsertFadD
UseTags6x HisExpressionBacterialMutationPromoterT7 lacOAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2C
Plasmid#15779DepositorInsertUbiquitin conjugating enzyme UbE2C (UBE2C Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-Tn5-F
Plasmid#160081PurposeBacterial expression of C-terminally FLAG-tagged Tn5 transposaseDepositorInsertTn5 transposase
UseTagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mCherry-hphMX6
Plasmid#105156PurposeYeast gene targetingDepositorInsertmCherry-hphMX6
UseTagsmCherryExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAT-WT-Myc
Plasmid#177717PurposeER protein alpha-1-antitrypsin (WT), myc taggedDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
UseTagsMycExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET GFP LIC cloning vector (2GFP-T)
Plasmid#29716DepositorTypeEmpty backboneUseTags6xHis, SiriusGFP, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET32bplus_trxAChEwt
Plasmid#83916PurposeEncodes an E. coli codon optimized version of wild type human acetylcholinesteraseDepositorInsertShort form of hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
UseTagsHis, S-tag, and TRXExpressionBacterialMutationcodon optimised for E.coli expressionPromoterT7 promoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only
