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pComb3XTT

Plasmid

#63891

Purpose

Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

Depositor

Carlos Barbas

Publication

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Insert

TT Fab

Use

Phage display

Tags

6xHis, HA tag, and geneIII

Expression

Bacterial

Promoter

lacZ

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pRsetB-pHRed

Plasmid

#31472

Depositor

Gary Yellen

Publication

Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

Insert

pHRed

Tags

7xHis

Expression

Bacterial

Available since

July 21, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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RFP-zyxin

Plasmid

#26720

Depositor

Anna Huttenlocher

Publication

Bhatt et al J Cell Sci. 2002 Sep 1. 115(Pt 17):3415-

Insert

Zyxin (ZYX Human)

Tags

DsRed

Expression

Bacterial and Mammalian

Available since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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SpyCatcher003 S49C

Plasmid

#133448

Purpose

Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

Depositor

Mark Howarth

Publication

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003 S49C

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Contains S49C mutation

Promoter

T7

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET259-pUC57 24xMS2V6

Plasmid

#104391

Purpose

MS2V6 cloning

Depositor

Robert Singer, Evelina Tutucci

Publication

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pGEMTEZ-TeTxLC

Plasmid

#32640

Depositor

Richard Axel, Joseph Gogos, C. Ron Yu

Publication

Yu et al Neuron. 2004 May 27;42(4):553-66.

Insert

Tetanus toxin light chain

Expression

Bacterial

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pET His6 MBP TEV LIC cloning vector (2M-T)

Plasmid

#29708

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, MBP, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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pET28a T7pCONS TIR-2 sfGFP

Plasmid

#154464

Purpose

pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Publication

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pJC175e

Plasmid

#79219

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PPSP gIII, luxAB

Expression

Bacterial

Promoter

PPSP

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMLRT

Plasmid

#78234

Purpose

Expresses Murine Leukemia Reverse Transcriptase (MLRT)

Depositor

Christopher Voigt

Publication

Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

Insert

Murine leukemia reverse transcriptase

Expression

Bacterial

Promoter

J23102

Available since

Aug. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pNCS-mRuby3

Plasmid

#74234

Purpose

Constitutive expression of mRuby3 in bacteria

Depositor

Michael Lin

Publication

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

mRuby3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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BPK764

Plasmid

#65767

Purpose

Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

Depositor

Keith Joung

Publication

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Bacterial

Promoter

T7 (x2)

Available since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTKRED

Plasmid

#41062

Depositor

Edward Cox, Thomas Kuhlman

Publication

Kuhlman et al Nucleic Acids Res. 2010 Apr;38(6):e92. E

Type

Empty backbone

Expression

Bacterial

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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FLAG-MATR3

Plasmid

#32880

Depositor

Yossi Shiloh

Publication

Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

Insert

MATR3 Matrin3 (MATR3 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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T4 Lysozyme WT

Plasmid

#18110

Depositor

Brian Matthews

Insert

T4 Lysozyme (e Bacteriophage T4)

Expression

Bacterial

Available since

Aug. 26, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJ4M/TDP-43

Plasmid

#104480

Purpose

bacterial expression of TDP-43

Depositor

Nicolas Fawzi

Publication

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP-43 (TARDBP Human)

Tags

MBP-6xHis

Expression

Bacterial

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pOpen_taq

Plasmid

#153315

Purpose

Bacterial expression of Taq polymerase

Depositor

Drew Endy

Insert

Taq polymerase

Expression

Bacterial

Promoter

Tac

Available since

June 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET-45b(+)-Tn5

Plasmid

#112112

Purpose

Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

Depositor

Frank Pugh

Publication

Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

Insert

Tn5

Tags

His6

Expression

Bacterial

Mutation

E54K E110K P242A L372P

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBB541

Plasmid

#27394

Depositor

Bernd Bukau

Publication

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

groESL (groL, groS E. coli)

Expression

Bacterial

Available since

March 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTP1112

Plasmid

#104158

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)

Depositor

Dirk Görlich

Publication

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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