29,779 results

1. pHH0103_GNAI2_G-alpha

   Plasmid

   #109791

   Purpose

   Protein expression and purification of GNAI2_G-alpha

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   GNAI2_G-alpha (GNAI2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM33_MDM2_SWIB

   Plasmid

   #178481

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   MDM2_SWIB (MDM2 Synthetic, Human)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFCiR5

   Plasmid

   #149479

   Purpose

   Broad-Host range expression vector, RSF1010 origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CinR(AM)-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PCin

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJL017

   Plasmid

   #53532

   Purpose

   This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

   Depositor

   James Fleckenstein

   Article

   Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

   Insert

   etpBA
   

   Use

   Tags

   6His and myc

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL030

   Plasmid

   #53533

   Purpose

   supplies EtpC required for optimal EtpA expression on compatible plasmid

   Depositor

   James Fleckenstein

   Article

   Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

   Insert

   etpC
   

   Use

   Pacyc184

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

6. R701-X71-527: His6-tev-Hs.CALM1(1-80)

   Plasmid

   #159694

   Purpose

   E. coli protein expression of His6-tev-Hs.CALM1(1-80)

   Depositor

   Dominic Esposito

   Article

   Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

   Insert

   His6-tev-Hs.CALM1(1-80) (CALM1 Human)
   

   Use

   Tags

   His6-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pgex-cIAP1 mut

   Plasmid

   #8334

   Depositor

   Jon Ashwell

   Article

   Li et al Nature 2002 Mar 21;416(6878):345-7.

   Insert

   ciap1mut (BIRC2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   H588A

   Promoter

   Available since

   Nov. 15, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pETDuet-AtRBX1-T7-AtSKP1-T7-AtCUL1-S

   Plasmid

   #238003

   Purpose

   Expression AtRBX1-T7, AtSKP1-T7 and AtCUL1-S in in bacterial cells

   Depositor

   Lei Wu

   Article

   Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

   Inserts

   AtRBX1 (RBX1 Mustard Weed)
   AtSKP1 (SKP1 Mustard Weed)
   AtCUL1 (CUL1 Mustard Weed)
   

   Use

   Tags

   S and T7

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYO1125

   Plasmid

   #235742

   Purpose

   Protein expression of GST-tagged PX domain (Cys at 2) in bacteria

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   p40 PX domain (NCF4 Human)
   

   Use

   Tags

   GST-TEV

   Expression

   Bacterial

   Mutation

   aa 2-149 with Cys at 2

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSF-MjCouRS

    Plasmid

    #229066

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    Methanococcus jannaschii tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGal-Trans-Myc-eGFP-KDEL

    Plasmid

    #177729

    Purpose

    ER protein collagen beta (1-O) galactosyltransferase fused to eGFP at the C-terminus, myc tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)
    

    Use

    Tags

    KDEL, Myc, and eGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. R701-X72-527: His6-tev-Hs.CALM1(76-148)

    Plasmid

    #159695

    Purpose

    E. coli protein expression of His6-tev-Hs.CALM1(76-148)

    Depositor

    Dominic Esposito

    Article

    Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

    Insert

    His6-tev-Hs.CALM1(76-148) (CALM1 Human)
    

    Use

    Tags

    His6-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHHM.X513-iNicSnFR3b-(V7)

    Plasmid

    #124881

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

    Depositor

    Henry Lester

    Article

    Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

    Insert

    iNicSnFR3b-(V7)
    

    Use

    Tags

    HA Tag, His Tag, and NA

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    T7

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBBR1MCS-2

    Plasmid

    #85168

    Purpose

    Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

    Depositor

    Kenneth Peterson

    Article

    Kovach et al Gene. 1995 Dec 1;166(1):175-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive (derepressed P-lac)

    Available since

    Nov. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pTP264

    Plasmid

    #149334

    Purpose

    Bacterial expression of Biotin ligase BirA

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Biotin ligase BirA
    

    Use

    Tags

    His14 and bdNEDD8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

    Plasmid

    #199390

    Purpose

    Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    14xHis-Avi-SUMOEu

    Expression

    Bacterial

    Mutation

    Promoter

    T5-LacO

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBBR1-kan-Ptet-RecETh1h2h3h4_agrob6

    Plasmid

    #180428

    Purpose

    Genome engineering of Agrobacterium

    Depositor

    Jun Fu

    Article

    Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

    Insert

    RecETh1h2h3h4_agrob6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28 His6-mEGFP-D4H

    Plasmid

    #226373

    Purpose

    Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

    Depositor

    Catherine Tomasetto

    Article

    Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

    Insert

    D4H (pfoA Clostridium perfringens)
    

    Use

    Tags

    His6 and monomeric EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCI-neo-cOVA

    Plasmid

    #25097

    Depositor

    Maria Castro

    Article

    Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

    Insert

    cytoplasmic ovalbumin
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMAL-AGR2

    Plasmid

    #18097

    Depositor

    Ronny Drapkin

    Insert

    AGR2 (AGR2 Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only