32,803 results
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Plasmid#222602PurposeExpression of MAC3-tagged GFP (N-terminal)DepositorInsertEGFP
TagsMAC3ExpressionBacterial and MammalianPromoterCMVAvailable SinceAug. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
O-FHL1-1
Plasmid#166543PurposescFv of a human scaffold targeting Four and a half LIM domains protein 1. Antigen coverage aa 1-280 aa, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbA15c-RFP
Plasmid#126108PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterJ23100Available SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD_sfGFP3TCG4TAG
Plasmid#174518PurposeRecoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.DepositorInsertsfGFP
Tags6 x HisExpressionBacterialMutationRecoded. TCG and TAG codons were introduced in po…PromoteraraBADAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
T-IL2-12
Plasmid#166564PurposescFv of a human scaffold targeting Interleukin-2. Antigen coverage aa 21-153 of 153, full-length excl. signal peptideDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, Sortag, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJH1295
Plasmid#36914DepositorInsert3x mCherry
ExpressionBacterial, Mammalian, and Y…Available SinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1_ATG4B_BamHI_NotI
Plasmid#190862PurposePlasmid for the expression and purification of of ATG4B. Internal reference: SMC1392DepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAT218
Plasmid#134886PurposeBacterial expressionDepositorInserteGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
FH
Plasmid#38978PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pN006
Plasmid#188470PurposeLentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)DepositorInsertAsCas12a
UseLentiviralExpressionBacterial and MammalianAvailable SinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCpf1b
Plasmid#122187PurposeBacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection markerDepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET Trx His6 LIC cloning vector (2Tc-T)
Plasmid#37239DepositorTypeEmpty backboneTags6xHis and Thioredoxin (Trx)ExpressionBacterialPromoterT7Available SinceAug. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS G12V
Plasmid#111850PurposeExpresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G12V (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 12 to ValinePromoterT5Available SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-EGFP
Plasmid#177136PurposeBacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFPDepositorInsertEGFP
ExpressionBacterialPromoterTacAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSHDY
Plasmid#137661PurposeConjugative vector with streamlined cloning capabilities. Contains two cloning sites for modular insertion of genetic parts.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFNR5
Plasmid#149483PurposeBroad-Host range expression vector, RSF1010 origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertNahR(AM)-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPSalTTCAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a Lm-iPGM
Plasmid#180242PurposeBacterial expression plasmid for production of recombinant Leishmania mexicana iPGM His10DepositorInsertLeishmania mexicana iPGM-10His
TagsHisExpressionBacterialPromoterT7Available SinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b T7pCONS TIR-2 sfGFP
Plasmid#154468PurposepET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSU20
Plasmid#173829PurposeExpresses 20 kD Penn State ladder proteinDepositorInsertSTRHSTPABPACCBPv3
ExpressionBacterialPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pet21_TurboID_18A
Plasmid#170922PurposeExpress TurboID_18A in Bl21DepositorInsertTurboID-18a
ExpressionBacterialAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTW334i
Plasmid#237319Purposeexpresses A. thaliana Cpn60α Cpn60β Cpn20 Raf2 RbcX Bsd2 N. tabacum Raf1DepositorInsertA. thaliana Cpn60α Cpn60β Cpn20 Raf2 RbcX Bsd2 N. tabacum Raf1
ExpressionBacterialAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2M
Plasmid#15798DepositorAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBroad-T7RNAP-chlorR
Plasmid#199992PurposeT7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) chlorRDepositorInsertT7RNAP Circuit
ExpressionBacterialMutationcopFnullAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNCS mHoneydew
Plasmid#91760PurposeBacterial expression of a fluorescent protein, monomeric Honeydew (mHoneydew)DepositorInsertmonomeric Honeydew
Tagsno tagsExpressionBacterialPromoterT7Available SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJMP2834
Plasmid#160673PurposeMobile CRISPRi sfGFP 'test', gmc6 sgRNADepositorInsertgmc6 (gfp) sgRNA
ExpressionBacterialAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-antiMouseMHCIINb
Plasmid#198292PurposeProduces PVCs retargeted with VHH7 anti-mouse MHC class II nanobodyDepositorInsertPVCpnf_pvc13-antiMouseMHCIINb
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mut1-RS
Plasmid#182881PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut1-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertMut1-RS
ExpressionBacterialAvailable SinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD461
Plasmid#178198PurposeRok with a C-terminal His-tag overexpressionDepositorInsertrok 6xHis
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBADZ-HisCre
Plasmid#111187PurposeHelper plasmid containing Cre-recombinase gene, which is expressed in DH10MultiBac cellsDepositorInsertCre recombinase
TagsHis tagExpressionBacterialAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRT498-OtUBD(V203D)
Plasmid#190090PurposeExpresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsertOtUBD(V203D)
Tags6xHis and MBPExpressionBacterialAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only