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pET20b-delta133HtrA2

Plasmid

#14126

Depositor

L. Miguel Martins

Article

Martins et al J Biol Chem. 2003 Dec 5. 278(49):49417-2

Insert

HtrA2 (HTRA2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 7, 2007

Availability

Academic Institutions and Nonprofits only

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pRG/III-hs-MBP-CB2-HF

Plasmid

#121682

Purpose

Bacterial expression of cannabinoid receptor CB2 fusion protein

Depositor

Reinhard Grisshammer, Richard Henderson

Article

Calandra et al Biotechnology Letters

Insert

peripheral cannabinoid receptor (CNR2 Human)

Use

Tags

His6 Flag tag

Expression

Bacterial

Mutation

Promoter

lac

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM4Z-OMA1

Plasmid

#61718

Purpose

human Oma1 protease in the E.coli/in vitro translation vector

Depositor

Oleh Khalimonchuk

Insert

OMA1 (OMA1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only
pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635

Plasmid

#91918

Purpose

Expresses LbfCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LbfCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pET-28-Strep-OPA1(253-960) (SB263)

Plasmid

#227612

Purpose

Inducible expression of Strep-tagged Opa1(253-960) in E.coli

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

OPA1 (OPA1 Human)

Use

Tags

Twin-StrepTag

Expression

Bacterial

Mutation

contains aa 253-960 only

Promoter

T7

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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WDV-Echistatin

Plasmid

#226985

Purpose

Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

Depositor

Wendy Gordon

Article

Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

Inserts

WDV (repA )
Echistatin

Use

Tags

6xHis and SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQE81XN-msYFP

Plasmid

#218960

Purpose

Expresses Hisx6-Tagged msYFP in bacteria

Depositor

Benjamin Glick

Insert

msYFP

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

T5

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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PJEBAN3

Plasmid

#203682

Purpose

Expresses Yellow Fluorescent Protein (YFP) in Listeria monocytogenes

Depositor

Tine Rask Licht

Article

Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

Insert

Yellow Fluorescent Protein (YFP)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRSETB/mStayGold

Plasmid

#212017

Purpose

mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

mStayGold

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLacO-ISce1

Plasmid

#58505

Purpose

provides 256 Lac operator repeats adjacent to the ISce1 restriction site

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Lac repeats adjacent to 6 copies of the ISce1 restriction site

Use

Tags

Expression

Bacterial

Mutation

Promoter

no promoter

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p-PDI

Plasmid

#202486

Purpose

Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Budding Yeast, Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

EM7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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N8his-GFPenhancer-GGGGS4-LaG16

Plasmid

#140442

Purpose

We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

Depositor

Motoyuki Hattori

Article

Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

Insert

GFPenhancer-GGGGS4-LaG16

Use

Tags

8 histidine tag

Expression

Bacterial

Mutation

Promoter

T7 promotor

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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NylCK-TS_pET28a

Plasmid

#215423

Purpose

Expression construct for NylCK-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCK-TS

Use

Tags

Expression

Bacterial

Mutation

S111G/A137L

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP1039

Plasmid

#119239

Purpose

expresses Tn7 transposase

Depositor

Oren Rosenberg, Jason Peters, Carol Gross

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

tnsABCD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pDisCoTune

Plasmid

#173713

Purpose

Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.

Depositor

Morten Norholm

Article

Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2

Inserts

ERV1 (ERV1 Budding Yeast)
PDI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pNeae2

Plasmid

#168300

Purpose

Bacterial surface display of polypeptides (nanobodies) in E. coli

Depositor

Luis Ángel Fernández

Article

Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:

Insert

Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus

Use

Tags

6xHis, E-tag, and myc-tag

Expression

Bacterial

Mutation

Promoter

LacI-Plac

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBA559

Plasmid

#186235

Purpose

pTet-LbuCas13a crRNA entry vector

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pPROEX Aqua

Plasmid

#42889

Depositor

Fabienne Merola

Article

Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

Insert

Aquamarine

Use

Tags

6xHis and TEV cleavage

Expression

Bacterial

Mutation

T65S and H148G

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pYAL_HLA-A2_MART1

Plasmid

#171177

Purpose

Displays MART-1 peptide in HLA-A*02 as a single-chain trimer

Depositor

Michael Birnbaum

Article

Huisman et al Methods Mol Biol. 2022;2491:263-291. doi

Insert

MART1_HLA-A2*01 single-chain trimer fusion

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

GAL1

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSF1389

Plasmid

#104962

Purpose

Bacterial expression plasmid of bdSENP1 protease

Depositor

Dirk Görlich

Article

Frey et al J Chromatogr A. 2014 Apr 11;1337:95-105.

Insert

SENP1

Use

Tags

His14 tag and Tev protease cleavage site

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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