30,249 results

1. pB18cmBFPAzurite

   Plasmid

   #14034

   Depositor

   Patrick Daugherty

   Article

   Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

   Insert

   Azurite
   

   Use

   Yeast/bacteria optimized codons

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEMG-AIS

   Plasmid

   #225133

   Purpose

   For genome editing in P. putida KT2440, arabinose is used for the induction of I-sceI gene. Km.

   Depositor

   Lars Blank

   Article

   Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. ilux pGEX(-)

   Plasmid

   #107879

   Purpose

   Bacterial expression of ilux for bioluminescence imaging

   Depositor

   Stefan Hell

   Article

   Gregor et al Proc Natl Acad Sci U S A. 2018 Jan 30;11

   Insert

   ilux
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX4T-1_WT_OCT4

   Plasmid

   #40633

   Depositor

   James Thomson

   Article

   Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

   Insert

   POU5F1 (POU5F1 Human)
   

   Use

   Tags

   GST, TEV cleavage, and Thrombin cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGJJ045

   Plasmid

   #183621

   Purpose

   abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.

   Depositor

   Ben Lehner

   Article

   Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT7-7 asyn WT

   Plasmid

   #36046

   Depositor

   Hilal Lashuel

   Article

   Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTW160C

   Plasmid

   #194801

   Purpose

   PCadBA3 expressing LuxAB

   Depositor

   Tina Wang

   Article

   Lee et al ACS Synth Biol. 2022 Oct 21;11(10):3198-

   Insert

   LuxAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBL069

   Plasmid

   #194800

   Purpose

   cCadC-GCN4 under the control of Ptet

   Depositor

   Tina Wang

   Article

   Lee et al ACS Synth Biol. 2022 Oct 21;11(10):3198-

   Insert

   cCadC-GCN4
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBL069B

   Plasmid

   #194802

   Purpose

   cCadC-GCN4* under the control of Ptet

   Depositor

   Tina Wang

   Article

   Lee et al ACS Synth Biol. 2022 Oct 21;11(10):3198-

   Insert

   cCadC-GCN4*
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHH0103_GNAI3_G-alpha

    Plasmid

    #110014

    Purpose

    Protein expression and purification of GNAI3_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNAI3_G-alpha (GNAI3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX-6P-1 Stu2 1-590

    Plasmid

    #38314

    Depositor

    Anthony Hyman

    Article

    Widlund et al Mol Biol Cell. 2012 Sep 19.

    Insert

    5' 1770 bp from STU2 ORF (STU2 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLibacceptorV2

    Plasmid

    #106250

    Purpose

    Recombination-mediated cassette exchange with target genomic loci

    Depositor

    Sriram Kosuri

    Article

    Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KLC2

    Plasmid

    #40735

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    KLC2 (KLC2 Human)
    

    Use

    Tags

    6xHis TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. SP-Cas9

    Plasmid

    #62731

    Purpose

    Expresses SP-Cas9 protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    SP-CAS9
    

    Use

    CRISPR

    Tags

    His tag and NLS

    Expression

    Bacterial

    Mutation

    Codon optimazed for e.coli

    Promoter

    T7

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRAN473

    Plasmid

    #120813

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

    Insert

    red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBL090J

    Plasmid

    #195045

    Purpose

    cCadC-CCB3.5 under the control of PBAD

    Depositor

    Tina Wang

    Article

    Lee et al ACS Synth Biol. 2022 Oct 21;11(10):3198-

    Insert

    cCadC-CCB3.5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNCST-AvicFP1

    Plasmid

    #129504

    Purpose

    Expresses AvicFP1 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AvicFP1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNCST-AvicFP4

    Plasmid

    #129507

    Purpose

    Expresses AvicFP4 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AvicFP4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    One of two variants of AvicFP4 tested with essent…

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-DAXX-ΔD/E-His

    Plasmid

    #179618

    Purpose

    Express GST-DAXX-ΔDE-His in bacteria

    Depositor

    Xiaolu Yang

    Article

    Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

    Insert

    death domain associated protein (DAXX Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deleted amino acids 424-495

    Promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNCST-AvicFP2

    Plasmid

    #129505

    Purpose

    Expresses AvicFP2 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AvicFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge