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Showing: 8451 - 8500 of 29646 results

  2. pAtCHYb1Δ129N

    Plasmid
    #53367
    Purpose
    Contains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.
    Insert
    chyB1 (BETA-OHASE 1 Mustard Weed)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Deleted codons for the first 129 amino acids
    Promoter
    Trc
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    53367-plasmid-map-sequence-id-109672

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  3. YARS_pNIC-CTB10H

    Plasmid
    #153059
    Purpose
    Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    YARS (YARS1 Human)
    Use
    Tags
    TEV-Avi-His10
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153059-plasmid-map-sequence-id-304479

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  8. pHybr_r2a>mG2a-srt-his

    Plasmid
    #124803
    Purpose
    HDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.
    Depositor
    Martijn Verdoes, Ferenc Scheeren
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG2a
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124803-plasmid-map-sequence-id-244318

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  12. RHOB

    Plasmid
    #38801
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RHOB (Rhob Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pJK615

    Plasmid
    #71699
    Purpose
    Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71699-plasmid-map-sequence-id-137034

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  14. pAV10.KN

    Plasmid
    #63209
    Purpose
    pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    63209-plasmid-map-sequence-id-115799

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  15. NM-01

    Plasmid
    #69799
    Purpose
    Bacterial expression of Cas9 with His, MBP, and Strep tags
    Depositor
    Gerard Cagney
    Insert
    Cas9
    Use
    Tags
    HIS, MBP, and StrepII
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine (D10A) and Histidine 840 …
    Promoter
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. CBX4

    Plasmid
    #25237
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX4_02 (CBX4 Human)
    Use
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. pCDF-mcherry2

    Plasmid
    #87145
    Purpose
    Constitutive E. coli mcherry Expression, with DAS4 tag
    Depositor
    Michael Lynch
    Insert
    mcherry
    Use
    Tags
    C terminal DAS4 tag
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive proD
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87145-plasmid-map-sequence-id-176838

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  20. pTXB1-hMeCP2

    Plasmid
    #48091
    Purpose
    Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-WT
    Depositor
    Huda Zoghbi
    Article
    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10
    Insert
    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mxe intein/chitin binding domain tagged
    Promoter
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. pJJS010

    Plasmid
    #188333
    Purpose
    PCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Article
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    Linker::mKate2::mIAA7
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188333-plasmid-map-sequence-id-370755

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  22. pJJS004

    Plasmid
    #188327
    Purpose
    PCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Article
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    Linker::mCherry::mIAA7
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188327-plasmid-map-sequence-id-370750

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  24. pJK633

    Plasmid
    #72397
    Purpose
    Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72397-plasmid-map-sequence-id-134629

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  25. pJK515

    Plasmid
    #72385
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide formyltransferase 2 (EcPurT)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylglycinamide formyltransferase 2
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72385-plasmid-map-sequence-id-133919

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  26. pES133

    Plasmid
    #127552
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Gavin Thomas, Emmanuele Severi
    Insert
    F5-flanked tetracycline resistance cassette
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Multiple SNPs in tetA (originally from pBR322) to…
    Promoter
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    127552-plasmid-map-sequence-id-251926

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  34. pE4c

    Plasmid
    #17464
    Purpose
    modified pENTR vector for C-terminal GST tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    GST
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    Promoter
    Available since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17464-plasmid-map-sequence-id-8632

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  38. RAB9B

    Plasmid
    #25625
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB9B (RAB9B Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. pGBW-m4046754

    Plasmid
    #145647
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145647-plasmid-map-sequence-id-292580

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  44. DinoIII

    Plasmid
    #128100
    Purpose
    Dinoflagellate expression vector backbone. XbaI and BglII restriction sites for gene insertion.
    Depositor
    Senjie Lin
    Article
    Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m
    Insert
    RNA complex & upstream/downstream of LHC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Inserts are potential promoters
    Available since
    April 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    128100-plasmid-map-sequence-id-292693

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  47. MYST1

    Plasmid
    #25180
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MYST1_01 (KAT8 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  48. pOGG010

    Plasmid
    #113984
    Purpose
    Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    RK2 (IncP)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113984-plasmid-map-sequence-id-223395

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Showing: 8451 - 8500 of 29646 results