32,671 results
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Plasmid#35045DepositorInsertSpyCatcher EQ
TagsHis6ExpressionBacterialMutationDeletion of C-terminal till I113, and point mutat…Available SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
Ec dnaN-pHK/P
Plasmid#237239Purposeover-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)DepositorInsertdnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
Tagshis-10+PKA kinase motif+PreScission Protease clea…ExpressionBacterialAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBWB514
Plasmid#209326Purposep15A-tetR-tet-SapI-ME-RBS-mRFP-ME-U1-Barcode-U2-SapI-dbI terminator-oriT-Cm. Fragment expression vector with Golden Gate sites.DepositorInsertmRFP
ExpressionBacterialPromotertetAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL2319
Plasmid#195697PurposeCryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaCDepositorInsertFoldon-MlaC
ExpressionBacterialMutation6xHis-Foldon-(Gly-Ser-Linker)-MlaCAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_BCAR1-1/1
Plasmid#91418PurposeProtein expression and purification of human SH3 domain construct BCAR1-1/1DepositorInsertBCAR1-1/1 (BCAR1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYTK080
Plasmid#65187PurposeEncodes ZeocinR as a Type 6 part to be used in the Dueber YTK systemDepositorInsertZeocinR
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
NylCA_pET28a
Plasmid#215418PurposeExpression construct for NylCA gene, codon optimized for expression in E. coliDepositorInsertNylCA
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylCp2_pET28a
Plasmid#215420PurposeExpression construct for NylCp2 gene, codon optimized for expression in E. coliDepositorInsertNylCp2
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-optimizedNedd4.2d
Plasmid#165103Purposebacterial expression of a GST fusion of Human Nedd4.2dDepositorInsertNedd4.2d (NEDD4L Human)
TagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expressionAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAM111
Plasmid#86481PurposeExpresses p9-6His-tnaADepositorInsertp9-6His-tnaA
UseSynthetic BiologyTags6His and tnaAExpressionBacterialPromoterpBADAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_RBFOX2-Halo
Plasmid#175304PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJS183
Plasmid#134142PurposeBacterial expression of GFP-Ska1 and Ska2DepositorInsertHis-GFP-human Ska1; human Ska2
TagsGFPExpressionBacterialAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-pMJ915v2
Plasmid#88916PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRExpressionBacterialAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-Sangamo-HIS
Plasmid#40787Purposefor e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokIDepositorInsertTranscription Activator Like Effector Nuclease
UseTALENTagsC Terminal 6xHISExpressionBacterialMutation+63 C Terminus,Codon Optimized FokI EndonucleasePromoterT7Available SinceDec. 12, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST15 hSlp4-a
Plasmid#40046DepositorAvailable SinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
EC10161
Plasmid#154060PurposeLevel 0.5 Golden Gate backbone, containing loxP sites.DepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyExpressionBacterialPromoterN/AAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX6-81nmt1
Plasmid#105164PurposeYeast gene targetingDepositorInserthphMX6-81nmt1
Tags81nmt1ExpressionBacterial and YeastPromoter81nmt1Available SinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX6-41nmt1
Plasmid#105163PurposeYeast gene targetingDepositorInserthphMX6-41nmt1
Tags41nmt1ExpressionBacterial and YeastPromoter41nmt1Available SinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB2_mScarletI_mVenusNB-MrkHR113A
Plasmid#182292Purposeexpresses c-di-GMP mutant biosensor in E. coliDepositorInsertmVenusNB-MrkHR113A
TagsmVenusNB internal fusion to MrkH with a R113A poi…ExpressionBacterialMutationchanged arginine 113 to alanine, MrkH numbering …PromoterproCAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXBI-mclover-YTHDC1-FL
Plasmid#177121PurposeBacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purificationDepositorInsertYTHDC1 (YTHDC1 Human)
TagsMxe GyrA intein-Chitin binding domain for protein…ExpressionBacterialPromoterT7Available SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pbacPuroR
Plasmid#26348DepositorInsertBacterial promoter::PuroR::bacterial 3p flank
ExpressionBacterialAvailable SinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
CBX7
Plasmid#25241PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBBR1_DVS_AF
Plasmid#188950PurposeLevel 2 destination vector containing pBBR1 origin of replication and CIDAR AF scarsDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19b+H2B(T116C)
Plasmid#157808PurposeExpression of human histone H2B with T116C mutation to facilitate maleimide chemistryDepositorInsertH2B(T116C)
ExpressionBacterialMutationT116CPromoterT7Available SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
htpG1-GFP
Plasmid#109387PurposeGFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG1-GFP
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_RRM2
Plasmid#157866Purposeexpress His tagged hnRNPA2 RRM2DepositorAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PIK3C2GA
Plasmid#42451PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
CD Ugi4
Plasmid#177196PurposeGreengate entry vector pGGC000 modified to contain bladderwort insulator 4DepositorInsertUtricularia gibba insulator 4
ExpressionBacterial and PlantAvailable SinceJune 28, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX6P1-DEST-Strep2
Plasmid#119753PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.DepositorTypeEmpty backboneTagsGST and Strep-tag II fusion tagExpressionBacterialPromotertac promoterAvailable SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
ibpAB-GFP
Plasmid#109390PurposeGFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertpibpAB-GFP
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
ALIX-His(1-868, P801G)
Plasmid#186808PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).DepositorInsertALIX-His (1-868, P801G) (PDCD6IP Human)
TagsHis6ExpressionBacterialMutationProline 801 to GlycineAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
DUSP16
Plasmid#38918PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
DDX3XA
Plasmid#42362PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKEK3395
Plasmid#234079PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertYukonOFP
ExpressionBacterialPromoterpJ23100-BsaIAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
Plasmid#198450PurposeProduces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10).DepositorInsertPVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
ExpressionBacterialAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 stat
Plasmid#112886PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertstat
Tags6xHisExpressionBacterialPromoterPblaAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLC133
Plasmid#198639PurposeExpression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.DepositorInsertdCas9
Tags3x-FLAGExpressionBacterialPromoterpTetAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
MacroH2A.2-c002
Plasmid#173089PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
MacroH2A1.2-c002
Plasmid#173088PurposeExpression of recombinant fusion protein in E. coliDepositorInsertMACROH2A1 (MACROH2A1 Human)
TagsmCeruleanExpressionBacterialMutationQ162-N372, codon optimizedAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pAN3944
Plasmid#74702PurposeCircuit plasmid: 0x1CDepositorInsertCircuit 0x1C
ExpressionBacterialAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
GABARAPL1
Plasmid#25215PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 12, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
human p53-(82-360)
Plasmid#24868DepositorAvailable SinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKPY514
Plasmid#62598PurposeEncodes Thr251Gly-EcPheRS Under IPTG-Inducible (PT5) ControlDepositorInsertThr251Gly-EcPheRS
Tags6xHisExpressionBacterialMutationThr251GlyPromoterT5Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRE141
Plasmid#118493Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
ExpressionBacterialAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pH6HTC_minus_cer-region
Plasmid#175297PurposeProduction of HaloTag fusion proteinDepositorTypeEmpty backboneUseCell free expressionTagsHaloTag-6xHisExpressionBacterialAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
PSIP1
Plasmid#40744PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceOct. 18, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSFDuet-1_VNp-Uricase
Plasmid#182401PurposeBacterial expression of Vesicle Nucleating peptide-uricase fusionDepositorInsertVNp-uricase
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only