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pET28a-SpyCatcher

Plasmid

#223633

Purpose

Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETB-roGFP1-R12

Plasmid

#83311

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Cannon et al Protein Sci. 2006 Jan;15(1):45-57. Epub

Insert

roGFP1-R12

Use

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/Q80R/S147C/Q204C/N149K/F223R/S202K

Promoter

T7

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pACYC-T7-Nla-repeat-BbsI

Plasmid

#180215

Purpose

For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-nsp7-nsp8

Plasmid

#159092

Purpose

Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

Depositor

Seth Darst

Article

Chen et al bioRxiv. 2020 Jul 13. doi: 10.1101/2020.

Inserts

NSP7
NSP8

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pWZ403

Plasmid

#208113

Purpose

mSwAP-In marker cassette 1 with mScarlet-PuroTK

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Insert

mScarlet-PuroTK

Use

Tags

P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1alpha

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMApra-Ptet2

Plasmid

#218361

Purpose

Empty backbone with tetR-PtetA expression system with two tetR operator sites

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pKM427

Plasmid

#210788

Purpose

L5 integrating vector for delivery of defective Hyg gene (ZeoR) for oligonucleotide-mediated SNP transfer

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2021;2314:301-321. doi

Insert

Defective hygromycin resistant gene

Use

Tags

Expression

Bacterial

Mutation

Changed hygromycin resistant gene W190 codon (TGG…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMJ841

Plasmid

#39318

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A) and Histidine 840 …

Promoter

T7

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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GST-p25

Plasmid

#24896

Depositor

Harish Pant

Article

Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

Insert

p25 (CDK5R1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

N-terminal truncated version of p35, contains aa …

Promoter

Available since

May 17, 2010

Availability

Academic Institutions and Nonprofits only
pET_RGG

Plasmid

#124929

Purpose

Expression of RGG domain from LAF-1 gene

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

LAF-1 RGG domain (laf-1 Nematode)

Use

Tags

His

Expression

Bacterial

Mutation

RGG domain

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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LacI-pLacI-pIV pm

Plasmid

#218101

Purpose

Phagemid that expresses LacI, and pIV under the Lac promoter

Depositor

Kevin Esvelt

Insert

pIV

Use

Tags

Expression

Bacterial

Mutation

Promoter

Lac promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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TOPO GFP

Plasmid

#68488

Purpose

TOPO construct expressing GFP for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Green Fluorescent Protein

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pProEx Rab5C(S30C) CL

Plasmid

#224273

Purpose

Protein purification of Rab5C (S30C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rab5C (RAB5C Human)

Use

Tags

Expression

Bacterial

Mutation

S30C

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCWori cytochrome P450-BM3h 9D7

Plasmid

#61308

Purpose

Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

Depositor

Alan Jasanoff

Article

Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

Insert

Cytochrome P450-BM3 heme domain 9D7

Use

Tags

6xHis

Expression

Bacterial

Mutation

T268A, I263A, T438V, A328G

Promoter

Tac

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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KG#62

Plasmid

#110874

Purpose

Expresses the C. elegans acy-1 cDNA pan-neuronally

Depositor

Kenneth Miller

Article

Reynolds et al Genetics. 2005 Feb;169(2):651-70. doi: 1

Inserts

rab-3 promoter
acy-1 cDNA
unc-54 3' control region with 1 intron just upstream and 1 intron in control region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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PET-HiFi SpCas9-NLS-6xHis

Plasmid

#207376

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HiFi SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R691A

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-P2A-EGFP_mRFPstuf

Plasmid

#137730

Purpose

Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA

Depositor

Fredrik Wermeling

Article

Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

Type

Empty backbone

Use

CRISPR, Lentiviral, Mouse Targeting, and Syntheti…

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA

Plasmid

#108549

Purpose

Bacterial Target-AID vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

dCas9-PmCDA1

Use

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

lambda OR

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBA562

Plasmid

#186237

Purpose

pTet-RfxCas13d crRNA entry vector

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Nme2Cas9_pMCSG7

Plasmid

#119925

Purpose

Nme2Cas9 bacterial expression plasmid

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

Nme2Cas9

Use

Tags

NLS, Nucleoplasmin NLS, and SV40 NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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