30,535 results

1. pet26-MBP-ELIC

   Plasmid

   #39239

   Depositor

   Raimund Dutzler

   Article

   Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

   Insert

   Eriwinia chrysanthemi - pentameric ligand gated ion channel
   

   Use

   Tags

   His-10 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET23a-H6-TEV-cAbl

   Plasmid

   #214234

   Purpose

   Bacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

   Depositor

   Neel Shah

   Article

   Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

   Insert

   ABL1 Kinase domain (ABL1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p40phox-PX (1-144)

   Plasmid

   #119123

   Purpose

   Bacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)

   Depositor

   Brett Collins

   Article

   Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

   Insert

   p40phox-PX (1-144)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBLxG2

   Plasmid

   #149472

   Purpose

   Broad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, GFPmut3b, Kanamycin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   LuxR-GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLuxB

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOSV806

   Plasmid

   #126606

   Purpose

   modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

   Depositor

   Jean-Luc Pernodet

   Article

   Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKCyR6

   Plasmid

   #149493

   Purpose

   Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Spectinomycin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CymR(AM)-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PCymRC

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMG023 MBP-Axin 384-518-His (miniAxin)

   Plasmid

   #154012

   Purpose

   Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   Axin1 (AXIN1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   381-518 fragment

   Promoter

   Tac

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSET.HaloTag.v7.CoOp.Cys-less

   Plasmid

   #195487

   Purpose

   Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Insert

   HaloTag.v7
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   C61T, C262V

   Promoter

   T7

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSF_his6_sumo_SARS_CoV2_NSP14_D90A/E92A

   Plasmid

   #213527

   Purpose

   Expresses NSP14 of SARS-CoV-2 with two point mutations D90A and E92A

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   NSP14 of SARS-CoV-2 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   D90A and E92A

   Promoter

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSF_his6_sumo_OC43_NSP14

    Plasmid

    #213519

    Purpose

    Expresses NSP14 of hCoV OC43

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV OC43 NSP14 (ORF1ab hCoV OC43)
    

    Use

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSF_his6_sumo_NL63_NSP14

    Plasmid

    #213520

    Purpose

    Expresses NSP14 of hCoV NL63

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV NL63 NSP14 (ORF1ab hCoV NL63)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSF_his6_sumo_229E_NSP14

    Plasmid

    #213521

    Purpose

    Expresses NSP14 of hCoV 229E

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV 229E NSP14
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRAR074-control-sfGFP

    Plasmid

    #185032

    Purpose

    J23108 promoter, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides of rpmE 5'UTR scrambled, Only fi…

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPSU10-30-50-100

    Plasmid

    #173838

    Purpose

    Coexpresses 10, 30, 50 and 100 kD Penn State ladder proteins

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-29b(+)_NSP4ss_SerM_NucA-His

    Plasmid

    #218190

    Purpose

    Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

    Depositor

    Florence Pojer

    Insert

    nucA
    

    Use

    Tags

    His and NSP4 signal secretion peptide

    Expression

    Bacterial

    Mutation

    codon optimized ecoli residues - 22-266

    Promoter

    T7

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSETB/mStayGold

    Plasmid

    #212017

    Purpose

    mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pComb3XSS

    Plasmid

    #63890

    Purpose

    3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment

    Depositor

    Carlos Barbas

    Article

    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

    Inserts

    SS Stuffer
    Light Chain Stuffer
    Heavy Chain Stuffer
    

    Use

    Phage display

    Tags

    6x His, HA tag, and geneIII

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSIJ8

    Plasmid

    #68122

    Purpose

    Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

    Depositor

    Alex Nielsen

    Article

    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

    Inserts

    flp recombinase
    rhaS
    rhaR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhamnose

    Available Since

    Dec. 8, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGEX-4T-1-3xFLAG-HSP27

    Plasmid

    #129229

    Purpose

    Encodes FLAG tagged human HSP27 for purification by GST affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    HSP27 (HSPB1 Human)
    

    Use

    Tags

    FLAG and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX6P1-mCherry-Nanobody

    Plasmid

    #70696

    Purpose

    Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    mCherry-Nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge