30,145 results

1. pJL1-sfGFP

   Plasmid

   #102634

   Purpose

   In vitro expression of sfGFP from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   sfGFP
   

   Use

   Tags

   strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBB540

   Plasmid

   #27393

   Depositor

   Bernd Bukau

   Article

   de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

   Insert

   grpE, clpB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE30-His-T7RNAP

   Plasmid

   #124138

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   T7 RNA polymerase (T7p07 Enterobacteria phage T7)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

   Plasmid

   #199371

   Purpose

   Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   anti-ALFA nanobody
   

   Use

   Tags

   14xHis-Avi

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T5-LacO

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDB179-ThermoPETase

   Plasmid

   #218698

   Purpose

   Encodes codon optimized ThermoPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   ThermoPETase
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMBP-AsCas12a

   Plasmid

   #113430

   Purpose

   Expresses 10xHis-MBP fused to AsCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   AsCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28 His6-mEGFP-D4H

   Plasmid

   #226373

   Purpose

   Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

   Depositor

   Catherine Tomasetto

   Article

   Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

   Insert

   D4H (pfoA Clostridium perfringens)
   

   Use

   Tags

   His6 and monomeric EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD-mTagBFP2

   Plasmid

   #34632

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   mTagBFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   I174A compared to mTagBFP (enhanced photostabilit…

   Promoter

   Available since

   Jan. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-HisMBP

   Plasmid

   #11085

   Depositor

   David Waugh

   Article

   Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac (lactose/IPTG inducible)

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. S100A6

    Plasmid

    #196217

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses S100A6.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    S100A6 (S100A6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBB542

    Plasmid

    #27395

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    dnaK, dnaJ, groESL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT-HA-rM3D(Gs)

    Plasmid

    #45549

    Purpose

    Expresses rM3D (Gs) neuronal activator

    Depositor

    Bryan Roth

    Article

    Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

    Insert

    rM3D (Gs)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEVOL_PylRS(WT)-tRNA

    Plasmid

    #223512

    Purpose

    PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

    Depositor

    Edward Lemke

    Article

    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

    Inserts

    Pyrrolysyl-tRNA Synthetase (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD, rrnB, and glnS and proK

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAD33.1

    Plasmid

    #36267

    Depositor

    Christian Raetz

    Article

    Chung et al Biochemistry. 2010 May 18;49(19):4126-37

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET29a-YS14

    Plasmid

    #66890

    Purpose

    Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

    Depositor

    Jung-Hyun Min

    Article

    Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

    Inserts

    His6-Thrombin-Xenopus laevis histones H2A
    Xenopus laevis histones H2B
    Xenopus laevis histones H3
    Xenopus laevis histones H4-Thrombin
    

    Use

    Tags

    H2A, H2B, H3, H4, His6 tag, and Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMal-T-Avi-His/BirA

    Plasmid

    #102962

    Purpose

    Vector for in vivo biotinylation of a fusion protein in bacteria.

    Depositor

    Tonia Rex

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD-HisD-miniGFP2

    Plasmid

    #188897

    Purpose

    Express 6xHis-Tag miniGFP2 in E.coli

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBad-PhoCl2c

    Plasmid

    #164035

    Purpose

    Improved PhoCl variant with enhanced dissociation efficiency

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    PhoCl2c
    

    Use

    Tags

    His tag, T7 tag, Xpress Tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBad promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. His6-pAG-RTase

    Plasmid

    #214932

    Purpose

    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems

    Depositor

    Chuan He

    Article

    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s

    Insert

    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…

    Promoter

    T7 promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid

    #212829

    Purpose

    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.

    Depositor

    Wolfgang Enard

    Article

    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

    Insert

    ZIM3-KRAB-dCas9-P2A-mCherry
    

    Use

    Tags

    HA-tag and ZIM3-KRAB

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG, TetOn

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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