34,213 results
-
Plasmid#103058Purposemedium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the hydrogen peroxide-inducible promoter katGpDepositorInsertGFP-OVA
ExpressionBacterialPromoterhydrogen peroxide-inducible promoter katGpAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNO4
Plasmid#101066PurposeBphP1-PpsR2 reporter plasmid; PBr_crtE-sfGFPDepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA-32Ala9
Plasmid#102616PurposeExpression of human RPA with an alanine substituted phosphorylation domain (with the following mutations in RPA32 :S8,11-13,23,29,33,39A,T21A).DepositorExpressionBacterialMutationwith an alanine substituted phosphorylation domainPromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pVC-Ds-MCS-Ds
Plasmid#102416PurposeEmpty vector with a multiple cloning site flanked by Ds integration elementsDepositorTypeEmpty backboneExpressionBacterialAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21 gamma-tubulin
Plasmid#101825Purposeproduction of gamma-tubulin in bacteriaDepositorInsertgamma-tubulin 1 (TUBG1 Human)
TagsHisExpressionBacterialMutationWhole proteinPromoterT7Available sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AcrIIA2
Plasmid#101042PurposeAnti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.DepositorInsertAcrIIA2
TagsGSTExpressionBacterialAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET21-Streptavidin-K121R
Plasmid#89880PurposeStreptavidin that has unimpaired biotin binding after dye labelingDepositorInsertStreptavidin-K121R-Glutamate_Tag
Tags6 glutamate tag (C terminal on insert)ExpressionBacterialMutationK121RAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm_P1T_DP-GH023
Plasmid#90317PurposeIntegration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023DepositorTypeEmpty backboneExpressionBacterialAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKD233.7-3
Plasmid#90955PurposeExpresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutivelyDepositorInsertsTetrathionate response regulator
Superfolder GFP
mCherry
ExpressionBacterialPromoterBba_J23114, PltetO-1, and PttrB185-269Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
ReNL_pRSETb
Plasmid#89536PurposeRed color variant of bright luminescent protein for bacterial expressionDepositorInsertReNL
TagsHis X 6ExpressionBacterialPromoterT7Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET21b-Caspase-3(V266E)
Plasmid#90089PurposeExpresses caspase 3 V266E mutant in bacterial cellsDepositorAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET14-Encap
Plasmid#86405PurposeExpresses T. maritima encapsulin in E. coliDepositorInsertEncapsulin
ExpressionBacterialPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(pos15)GFP
Plasmid#89248Purpose[Edit] Expresses GFP with +15 theoretical charge in E.ColiDepositorInsertHis6-(pos15)-GFP
TagsHis6ExpressionBacterialPromoterT7Available sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLZ12Km2-P23R:TA:ffluc
Plasmid#88900PurposeToxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expressionDepositorInserttoxin-antitoxin + firefly luciferase
UseLuciferaseExpressionBacterialAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ZIKV_Sensor_32B_LacZ
Plasmid#75007PurposeToehold switch sensor to detect Zika VirusDepositorInsertZIKV_sensor_32B_LacZ
TagsLacZExpressionBacterialPromoterT7Available sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCali2
Plasmid#86119PurposeMultiplex temperature-gated expression of mWasabi and mCherry reportersDepositorInsertsTlpA36
mWasabi
TcI
mCherry
Tags6xHisExpressionBacterialMutationA67T and D341A, P60L, D135V, K187R, K202I, L208QPromoterpR/pL, pR/pL, pLacI, pTlpA, and pTlpA, pLacIAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTcI42-Wasabi
Plasmid#86118PurposemWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42DepositorInsertsTcI42
mWasabi
Tags6xHisExpressionBacterialMutationA67T, K6N, S33T, Y61H, L119P, F122CPromoterpR/pL and pR/pL, LacIAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A
Plasmid#83484PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).DepositorInsertLbu_C2c2_R1048A_H1053A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR1048A, H1053AAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-roGFP1-R12
Plasmid#83311PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-R12
Tags6xHISExpressionBacterialMutationC48S/Q80R/S147C/Q204C/N149K/F223R/S202KPromoterT7Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDF-mTET1
Plasmid#81052Purposebacterial expression of murine TET1DepositorAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by