29,747 results

1. lacI-PT7-gusA-pBAV1k

   Plasmid

   #30504

   Depositor

   Ichiro Matsumura

   Article

   Murin et al Appl Environ Microbiol. 2011 Oct 21.

   Insert

   beta-glucoronidase (gusA Escherichia coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pgRNAtet-lvaA

   Plasmid

   #106397

   Purpose

   Guide RNA plasmid targeting lvaA on BBR1-UP origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   sgRNA towards lvaA in Pseudomonas putida
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMRE-Tn5-145

   Plasmid

   #118529

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mScarlet-I
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBad-HisB-RRvT

   Plasmid

   #87364

   Purpose

   Expresses RRvT, a tandem heterodimer red fluorescent protein

   Depositor

   Robert Campbell

   Article

   Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

   Insert

   RRvT
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBad (Arabinose)

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSFDuet1-Cdc45

   Plasmid

   #126878

   Purpose

   Expresses human Cdc45 in bacteria

   Depositor

   Luca Pellegrini

   Article

   Simon et al Nat Commun. 2016 May 18;7:11638. doi: 10

   Insert

   CDC45 (CDC45 Human)
   

   Use

   Tags

   No tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFDH

   Plasmid

   #131706

   Purpose

   Express constitutively an NAD-dependent formate dehydrogenase from Pseudomonas sp. 101 in E. coli

   Depositor

   Ron Milo

   Article

   Gleizer et al Cell. 2019 Nov 27;179(6):1255-1263.e12.

   Insert

   formate dehydrogenase
   

   Use

   Tags

   his-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDSW1728

   Plasmid

   #120812

   Purpose

   E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)

   Depositor

   David S. Weiss, Craig Ellermeier

   Article

   Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

   Insert

   red fluorescent protein (mCherry), codon-optimized for C. difficile
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-SpyCatcher

   Plasmid

   #223633

   Purpose

   Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   SpyCatcher003 S49C
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pETM33_Nsp5_Mpro

   Plasmid

   #156475

   Purpose

   Bacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp5_Mpro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. htpG1-GFP

    Plasmid

    #109387

    Purpose

    GFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    phtpG1-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTAT-CRE

    Plasmid

    #35619

    Depositor

    Steven Dowdy

    Article

    Wadia et al Nat Med. 2004 Mar;10(3):310-5. Epub 2004

    Insert

    CRE (cre Bacteriophage P1)
    

    Use

    Tags

    6xHIS, T7 tag, and TAT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pFW2000

    Plasmid

    #224211

    Purpose

    E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT2/BH

    Plasmid

    #26556

    Depositor

    Perry Hackett

    Insert

    pT2/BH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Nme2Cas9_pMCSG7

    Plasmid

    #119925

    Purpose

    Nme2Cas9 bacterial expression plasmid

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    Nme2Cas9
    

    Use

    Tags

    NLS, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-5X-3-SARS-CoV-2-3CL

    Plasmid

    #168457

    Purpose

    Bacterial codon optimized expression vector for SARS-CoV-2 3CL protease.

    Depositor

    David Ho, Alejandro Chavez, Sho Iketani

    Article

    Iketani et al Nat Commun. 2021 Apr 1;12(1):2016. doi:

    Insert

    SARS-CoV-2 3CL protease (ORF1ab Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pR_Lux

    Plasmid

    #226457

    Purpose

    lambda immunity region to the luciferase operon (lux)

    Depositor

    Emily Balskus

    Article

    Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

    Insert

    lambda immunity region to the luciferase operon (lux) from Photorhabdus luminescens
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBbB8c-GFP

    Plasmid

    #35362

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BAD

    Available since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOPINB-hRNF216(457-784)

    Plasmid

    #171922

    Purpose

    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic helix-RBR-helix construct suitable for activity assays.

    Depositor

    Bernhard Lechtenberg

    Article

    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

    Insert

    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    

    Use

    Tags

    3C protease cleavage site and 6x-His tag

    Expression

    Bacterial

    Mutation

    codon optimised for expression in E. coli

    Promoter

    T7

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBA562

    Plasmid

    #186237

    Purpose

    pTet-RfxCas13d crRNA entry vector

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBSV2_P0826-mCherryBb

    Plasmid

    #118242

    Purpose

    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0826 promoter driven mCherry

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    bb0826 promoter driven mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge