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34,213 results

Showing: 3921 - 3940 of 34213 results

  1. pSUB11

    Plasmid
    #169699
    Purpose
    Template plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Publication
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
    Tags
    3xFLAG tag
    Expression
    Bacterial
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169699-plasmid-map-sequence-id-336760

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  3. FHL-cpmTq2-Calcium-lifetime-sensor

    Plasmid
    #129628
    Purpose
    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes
    Depositor
    Dorus Gadella
    Publication
    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FHL-cpmTq2-Calcium-lifetime-sensor
    Tags
    6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …
    Expression
    Bacterial and Mammalian
    Promoter
    CMV,&Rhamnose
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129628-plasmid-map-sequence-id-260204

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  8. pExp-DsbC

    Plasmid
    #112564
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-DsbC and Strep tag
    Expression
    Bacterial
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112564-plasmid-map-sequence-id-217214

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  9. pExp-sfGFP

    Plasmid
    #112573
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis, Strep II, and sfGFP
    Expression
    Bacterial
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112573-plasmid-map-sequence-id-217227

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  10. pExp-Trx

    Plasmid
    #112577
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-TrxA and Strep tag
    Expression
    Bacterial
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112577-plasmid-map-sequence-id-217212

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  14. pCLASP_mScarlet_CLASP_RGS2membrane

    Plasmid
    #133086
    Purpose
    Plasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to bl
    Depositor
    Hana El-Samad
    Publication
    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2
    Insert
    mScarlet-CLASP
    Expression
    Bacterial and Yeast
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    133086-plasmid-map-sequence-id-296450

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  16. pET28-Nsp14-TEV-6His

    Plasmid
    #154952
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Nsp14-TEV-6His (ORF1ab SARS-CoV-2)
    Tags
    TEV-6His
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154952-plasmid-map-sequence-id-307820

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  17. pET28-6His-TEV-Nsp14

    Plasmid
    #154954
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    Tags
    6His-TEV
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    154954-plasmid-map-sequence-id-307815

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  18. pETM33_Nsp9

    Plasmid
    #156478
    Purpose
    Bacterial expression of Sars-CoV2 Nsp9 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Publication
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156478-plasmid-map-sequence-id-307616

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  20. pCgsgRNA_crtYf

    Plasmid
    #153338
    Purpose
    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr
    Depositor
    Sheng Yang
    Publication
    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19
    Insert
    crRNA targeting crtYf
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153338-plasmid-map-sequence-id-306018

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Showing: 3921 - 3940 of 34213 results