31,059 results
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Plasmid#187843PurposeConstitutive mNeonGreen-expression vector for Fusobacterium nucleatumDepositorInsertmNeonGreen
ExpressionBacterialAvailable SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
yTREX
Plasmid#177306Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5DepositorTypeEmpty backboneUseSynthetic Biology; Yeast expression, tn5 genomic …ExpressionBacterialAvailable SinceAug. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-AspS
Plasmid#159618PurposeExpression plasmid for AcpS phosphopantetheinyl transferaseDepositorInsertAcpS
Tags6HISExpressionBacterialAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCold-TF-hPLEKHM1
Plasmid#64146PurposeFor protein expression in E.coliDepositorInsertPLEKHM1 (PLEKHM1 Human)
Tags6xHis-tag and Trigger Factor (TF)ExpressionBacterialPromotercspAAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS Wnt-2HA
Plasmid#17368DepositorAvailable SinceJuly 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pWZ853
Plasmid#208115PurposemSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-BSDDepositorInsertmNeonGreen-HPRT1-BSD
TagsT2AExpressionBacterial and MammalianPromoterEF1alphaAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM43 p62 FL (1-440)
Plasmid#210425PurposeBacterial expression of MBP-p62 FL (1-440) with 3C cleavage siteDepositorAvailable SinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)
Plasmid#169186PurposeTo express His-SUMO-nsp7 (SARS-CoV-2) in E. coliDepositorInsert14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)
Tags14His-SUMO (Ulp1-cleavable)ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα12-LgB115
Plasmid#134363PurposeNanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-rIgG-Fc2-ICAM1
Plasmid#156462PurposeExpresses a rabbit Fc-ICAM1 fusion protein in mammalian cellsDepositorInsertIntercellular Adhesion Molecule 1 (Icam1 Mouse)
TagsICAM1-rFcExpressionBacterial and MammalianMutationendogenous signal peptide replaced with IL2 signa…PromoterhEF1-HTLCAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-ADARcd-E488Q-V5
Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Casp1-His
Plasmid#11809DepositorAvailable SinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHCL147
Plasmid#134454PurposeTetR, lacI-Plac-ssdsbA-mscarC, Integrative plasmid for the expression of fully-secreted prey. C-terminal mScarlet-I fusion.DepositorInsertlinker-mScarlet-I for C-terminal fusion
ExpressionBacterialPromoterplacAvailable SinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-KP (KanR, Phi80)
Plasmid#45986DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJAT13araE
Plasmid#18987DepositorAvailable SinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHTganA-Cpf1
Plasmid#158647PurposeConstitutive transcription of FnCpf1 and ganA crRNADepositorInsertCpf1, ganA crRNA
UseCRISPR and Synthetic Biology; Shuttle vector baci…ExpressionBacterialPromoterP43Available SinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pASPIre2
Plasmid#154843PurposeDerivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1DepositorInsertBxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
ExpressionBacterialAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKnockinDonor-human-p16INK4A-P2A-mCherry
Plasmid#137026Purposedonor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4ADepositorInsertp16INK4A-knockin-homology arm-P2A-mCherry
TagsP2A-mCherryExpressionBacterialPromoterno promoterAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
p005-RFP-strong
Plasmid#108317PurposeSynthetic RFP based on Corynactis spp under strong synthetic constitutive promoter pFAB4005; kanamycin resistant.DepositorInsertRFP under controlf of strong synthetic constitutive promoter pFAB4005
ExpressionBacterialPromoterpFAB4005Available SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMD14
Plasmid#138654PurposephiOZJ integrating Streptomyces expression vector, intact RDFDepositorTypeEmpty backboneExpressionBacterialPromoterPermE* variant sequence from pDA1652Available SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only