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SARS-CoV-2 Nucleocapsid

Plasmid

#242452

Purpose

Expresses the SARS-CoV-2 Nucleocapsid protein

Depositor

Benjamin Schuster

Article

Favetta et al bioRxiv [Preprint]. 2025 Jun 10:2024.10.

Insert

Nucleocapsid protein

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-TEV-HsFbxo7-129-398/Skp1

Plasmid

#202592

Purpose

Bacterial expression of HsFbxo7-129-398 and HsSkp1

Depositor

Brenda Schulman

Article

Kraus et al EMBO Rep. 2023 Aug 3;24(8):e56399. doi:

Inserts

Fbxo7 (FBXO7 Human)
Skp1 (SKP1 Human)

Tags

GST, TEV

Expression

Bacterial

Mutation

aa 129-398 only

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-AviTag-DogCatcher-MBP

Plasmid

#171928

Purpose

Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

AviTag-DogCatcher-MBP

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

DogCatcher added to N-terminus of MBP via a GSGES…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pB1H1

Plasmid

#12611

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

Insert

Dorsal RHR in pB1H1 (dl Fly)

Tags

flag

Expression

Bacterial

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pFA6a-CLIP-KanMX6

Plasmid

#87025

Purpose

Tag S. pombe genes in C terminal with CLIP

Depositor

Julien Berro

Insert

CLIP

Expression

Bacterial and Yeast

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCDF-5xT7-TtCsm

Plasmid

#128572

Purpose

For expression of T. thermophilus Csm in E. coli (Csm1-5)

Depositor

Jennifer Doudna

Article

Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

Inserts

Csm1
Csm2, Csm3, Csm4, Csm5

Tags

HRV 3C protease cleavage site and 10xHis tag on C…

Expression

Bacterial

Mutation

Codon optimized for expression in E. coli and Cod…

Promoter

T7 promoter, lac operator

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pDropVn

Plasmid

#184873

Purpose

Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pTriEx-NTOM20-mVenus-Zdk1

Plasmid

#81010

Purpose

Expression of Zdk1 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

Plasmid

#107581

Purpose

B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

Depositor

Paul Freemont

Article

Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Promoter

pdh promoter Bacillus megaterium DSM319

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMGE-T7

Plasmid

#153021

Purpose

Single-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.

Depositor

Vito Valiante

Article

Kufs et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

Plasmid

#172506

Purpose

Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 bacterial expression vector with golden gate site for guides

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSHS212

Plasmid

#87346

Purpose

Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/C574S

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCE35

Plasmid

#174409

Purpose

C. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.

Depositor

Clarissa Nobile

Article

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pWH1266-Apra-sgRNA

Plasmid

#218372

Purpose

sgRNA expression plasmid for CRISPR-intereference

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Insert

sgRNA expression cassette

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMBP-HIS2-Vps2

Plasmid

#21494

Depositor

James Hurley

Article

Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

Insert

VPS2 (DID4 Budding Yeast)

Tags

6x His and MBP

Expression

Bacterial

Mutation

none

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-4T1-USP11

Plasmid

#78849

Purpose

To purify GST-tagged recombinant USP11 protein

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2015 Sep;22(9):1463-7

Insert

USP11 (USP11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pQE-60NA-mScarlet-2I

Plasmid

#160463

Purpose

Encodes a variant of mScarlet-I that displays reduced cytotoxicity in bacteria

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

mScarlet-2I

Expression

Bacterial

Promoter

T5

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pREC1010-Eco1

Plasmid

#240680

Purpose

RSF1010 origin of replication plasmid containing Eco1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Eco1 RT, Eco1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pREC1010-Eas1

Plasmid

#240685

Purpose

RSF1010 origin of replication plasmid containing Eas1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Eas1 RT, Eas1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-FKP-ET64

Plasmid

#234665

Purpose

Expression an ELP-tagged FKP enzyme

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Insert

fucokinase/GDP-fucose pyrophosphorylase

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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