30,753 results

1. pAtetO_6xHis-Bst-LF

   Plasmid

   #145799

   Purpose

   Plasmid for the aTc-inducible expression of the large-fragment of Bst DNAP

   Depositor

   Andrew Ellington

   Article

   Bhadra et al mSphere. 2021 May 19;6(3):e00911-20. doi

   Insert

   Bst-LF
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   tet PA

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCDM4

   Plasmid

   #49796

   Purpose

   ePathBrick vector for pathway engineering in E. coli.

   Depositor

   Mattheos Koffas

   Article

   Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

   Type

   Empty backbone

   Tags

   S-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)

   Plasmid

   #174516

   Purpose

   Recoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Inserts

   PylS_1R26 - CBZK
   Af_TyrRS - p-I-Phe
   Ma tRNA pylT(8) with CGA anticodon
   A.fulgidus tRNA with CUA anticodon
   

   Expression

   Bacterial

   Mutation

   CUA anticodon, M.alvus Pyl tRNA with CGA anticodo…

   Promoter

   GlnS and lpp

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbB5k-GFP

   Plasmid

   #35354

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   lacUV5

   Available Since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBECKP-spe

   Plasmid

   #117236

   Purpose

   ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Insert

   ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZac2.1 gfaABC1D-Aqp4-BioID2-BioID2-HA

   Plasmid

   #176742

   Purpose

   Expresses Aquaporin-4 fused with BioID2 at the C-terminus in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   Aqp4-BioID2-BioID2-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Promoter

   gfaABC1D

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMW363

   Plasmid

   #238208

   Purpose

   Expresses TasR with an N-terminal Twin-StrepII tag, followed by 3xRNA1 TIGR array. AmpR

   Depositor

   Feng Zhang

   Article

   Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

   Insert

   TaTasR
   

   Tags

   TwinStrepII

   Expression

   Bacterial

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMW364

   Plasmid

   #238205

   Purpose

   Expresses untagged TasR from a PBAD promoter, followed by 3xRNA1 TIGR array

   Depositor

   Feng Zhang

   Article

   Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

   Insert

   TaTasR
   

   Expression

   Bacterial

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST17-UbE2T

   Plasmid

   #15808

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2T (UBE2T Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pET21b-Casp1-His

    Plasmid

    #11809

    Depositor

    Guy Salvesen

    Insert

    Caspase-1 (CASP1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept

    Plasmid

    #182424

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteins

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ-mNeongreen-Etanercept (TNFRSF1B Human)
    VNp-LZ
    

    Tags

    Vesicle Nucleating peptide (VNp), and Vesicle Nuc…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDD56

    Plasmid

    #119883

    Purpose

    Arabinose inducible EYFP on pAL5000/pMB1 backbone with kanamycin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    pBAD_EYFP
    

    Expression

    Bacterial

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXTK025 [Origin_RK2-oriT_PartType1]

    Plasmid

    #229262

    Purpose

    XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_RK2-oriT_PartType1

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Insert

    RK2-oriT Origin
    

    Expression

    Bacterial

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)

    Plasmid

    #218513

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferase

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Expression

    Bacterial

    Mutation

    deletion of the Plac_mrfp cassette

    Promoter

    PgyrA_ermBL-ermB'

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCR8-attL1-pegRNA-attR3

    Plasmid

    #213051

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    35S-CmYCLV-AtU6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-(SUMO)3

    Plasmid

    #127093

    Purpose

    Bacterial expression plasmid for eGFP-(SUMO)3 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    EGFP - 3 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
    

    Tags

    EGFP

    Expression

    Bacterial

    Promoter

    unknown

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-mGinger1

    Plasmid

    #119784

    Purpose

    Expresses mGinger1 tagged N-terminally with an MHHHHHHGSG tag

    Depositor

    Stephen Mayo

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

    Insert

    mGinger1
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTSlb-wt

    Plasmid

    #60728

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. HS-GFP-PACT

    Plasmid

    #105954

    Purpose

    heat shock inducible transgenesis, PACT is a centriol marker

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    PACT (AKAP9 Human)
    

    Tags

    GFP

    Expression

    Bacterial

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGSHU

    Plasmid

    #72244

    Purpose

    Contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter

    Depositor

    Michael Resnick, Francesca Storici

    Article

    Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

    Inserts

    KlURA3
    hyg
    I-SceI
    

    Expression

    Bacterial

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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