31,059 results
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Plasmid#61285PurposeConstitutive repeat-only E. coli CRISPR arrayDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterJ23119Available SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only
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SEPTOPO
Plasmid#64944Purposesource of SEP (Super Ecliptic pHluorin) sequenceDepositorInsertSEP
ExpressionBacterialPromoterlacAvailable SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046806
Plasmid#145746PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-4T-1-p19-T7-Vif
Plasmid#46313DepositorInsertsp19
Vif DNA-1
linker
Vif DNA-2
UsePro-sirnaTags6XHis and GSTExpressionBacterialAvailable SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 FlAsH LIC cloning vector (H6-FlAsH)
Plasmid#30184DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), FlAsH, and TEV cleavage s…ExpressionBacterialAvailable SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-H6-FGB
Plasmid#49203PurposeFluorescent reporter for cGMPDepositorInsertH6-FGB
TagsHis tag + Other tagsExpressionBacterial, Insect, and Mamm…MutationNonePromoterCMVAvailable SinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAB085d
Plasmid#79209PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F3
ExpressionBacterialPromoterPlacZ-opt (OR1); PtetAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylB_pET29b
Plasmid#215414PurposeExpression construct for NylB gene, codon optimized for expression in E. coliDepositorInsertNylB
ExpressionBacterialAvailable SinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
S9_attL1-EGFP-C-term-attL2
Plasmid#128473PurposeEGFP entry cloneDepositorInsertEGFP entry clone
ExpressionBacterialAvailable SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
L1-H210Q
Plasmid#15500DepositorAvailable SinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSA12
Plasmid#186546PurposeLSSA12 fluorescent protein (ex/em = 398/511 nm): bacterial expression plasmidDepositorInsertLSSA12
Tags6xHisExpressionBacterialMutationhfYFP-G65S/Y203I/Q204E/E222D/R223FPromoteraraBAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1
Plasmid#116963PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
ExpressionBacterialPromoterT7Available SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-156
Plasmid#118538PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmCardinal
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rha
Plasmid#129390PurposeDerived from mini-CTX1. An integrase vector that can integrate the rhamnose-inducible promoter system into the chromosome of a host bacterium.DepositorInsertRhamnose-inducible promoter
ExpressionBacterialMutationN/APromoterPrhaBADAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PI(WT)-Src(KD)-mCherry
Plasmid#87358PurposePI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutationDepositorInsertsTagsmCherryExpressionBacterial and MammalianMutationD388RPromoterCMVAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIB164
Plasmid#90187PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pami promoterDepositorTypeEmpty backboneTagsNoneExpressionBacterialAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-MBP-TEV-NAP1 (WT)
Plasmid#208871PurposeFor expression in E. coli of NAP1DepositorAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSP2283
Plasmid#70702PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialPromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMS18: pHelper(PmcCAST)_PmcPSP1
Plasmid#168151PurposeInducible expression of PmcCAST proteins with crRNA targeting PmcPSP1.DepositorInsertsPmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTKDP-cat
Plasmid#71321PurposeDonor plasmid - chloramphenicol resistant donor cassetteDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
WT-β3
Plasmid#166579PurposeFor expression of cytoplasmic tail of mouse beta3 integrin with N-terminal GST fusion protein in bacterial cells.DepositorAvailable SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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HERC2 (3KCI)
Plasmid#25344PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertherc2.3951.4321 (HERC2 Human)
TagsHisExpressionBacterialMutationcontains amino acid residues 3951-4321Available SinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXN-FBLWLF-LexA
Plasmid#26266DepositorInsertLexA
UseDrosophila transgenesisExpressionBacterial and InsectAvailable SinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBTRCk-pVhb-maACR
Plasmid#215651PurposepBBR1 Origin, KanR, with maACR gene under Vhb promoterDepositorInsertacyl-CoA reductase
TagsMaltose binding protein and His tagExpressionBacterialPromoterVhbAvailable SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-pVhb-vfFadBA
Plasmid#215650PurposepACYC Origin, CmR, with vfFadBA gene under Vhb promoterDepositorInsertthiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
ExpressionBacterialPromoterVhbAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK_ToeRepG2_N19_trigger
Plasmid#132747PurposeT7 RNAP-driven expression of second-generation toehold repressor index 19 trigger RNADepositorInsertToeRepG2_N19_trigger
ExpressionBacterialAvailable SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS
Plasmid#117389PurposeTn7 tagging vector with temperature-sensitive origin of replicationDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-AR-1-566
Plasmid#104195PurposeExpresses human AR-1-566 from bacterial expression vector pGEX-5X-1 as GST fusionDepositorAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas9-J23109
Plasmid#113150PurposePlasmid constitutively expressing Cas9 proteinDepositorInsertCas9
ExpressionBacterialAvailable SinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGSTag Flag Jnk2a2
Plasmid#15744DepositorInsertJnk2a2 (MAPK9 Human)
ExpressionBacterialAvailable SinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
KaiA
Plasmid#42497DepositorInsertKai A
TagsGSTExpressionBacterialPromotert7Available SinceFeb. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
DVL2
Plasmid#38876PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMS17: pTwinStrep-SUMO-AvTnsD
Plasmid#168150PurposeAvTnsD expression construct for protein purification.DepositorInsertAvCAST TnsD
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS15: pTwinStrep-SUMO-AvTnsB
Plasmid#168148PurposeAvTnsB expression construct for protein purification.DepositorInsertAvCAST TnsB
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS16: pTwinStrep-SUMO-AvTnsC
Plasmid#168149PurposeAvTnsC expression construct for protein purification.DepositorInsertAvCAST TnsC
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS14: pTwinStrep-SUMO-AvTnsA
Plasmid#168147PurposeAvTnsA expression construct for protein purification.DepositorInsertAvCAST TnsA
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSY14-ptb
Plasmid#90490PurposepSY8 derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in ClostridiumDepositorInsertsceC
ExpressionBacterialAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
HOXA7 (murine) His-tag pET
Plasmid#8491DepositorInsertHOXA7 (Hoxa7 Mouse)
TagsHis and T7ExpressionBacterialMutationFusion sequence is: ggatccATG; makes protein by …Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
Reb_65 aka PAS626
Plasmid#71231PurposeReb_1 with RebA(Y82A)DepositorInsertReb locus
TagsnoneExpressionBacterialMutationRebA(G53A, Y82A)Available SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-S9
Plasmid#132948PurposeExpresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coliDepositorInsert30S ribosomal subunit protein S9 (rpsI E.coli)
TagsN-terminal 6xHis-tagExpressionBacterialMutationE52CPromoterT7Available SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pQMD
Plasmid#48103PurposeDestination plasmid (Gateway cloning) for cumate-inducible expression of myc-tagged fusion proteinDepositorTypeEmpty backboneTagsmyc tagExpressionBacterialPromoterPQ5Available SinceJan. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
p2Lox
Plasmid#34635DepositorTypeEmpty backboneTagsEGFPExpressionBacterialPromoterPGK promoterAvailable SinceFeb. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A
Plasmid#83484PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).DepositorInsertLbu_C2c2_R1048A_H1053A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR1048A, H1053AAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-dHer1cys
Plasmid#73218PurposeBacterial expression vector for Z1907 affibody protein (A) with N-terminal His10, GST and HRV3C cleavage site (cysteine tag)DepositorInsert10XHis-GST-HRV-dHer1cys
Tags10XHis-GST-HRV and The dimer of the Her1 Affibody…ExpressionBacterialPromoterT7Available SinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK3_HUMAN_D0
Plasmid#79689PurposeThis plasmid encodes the kinase domain of STK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD*RFPEC2
Plasmid#17823DepositorInsertE. coli optimized red flourescent protein (RFPec2)
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-GST-eIF4G1-His6
Plasmid#37231DepositorAvailable SinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
PclpB GFP pZa
Plasmid#170074PurposeGFP expression under the control of E. coli clpB promoterDepositorInsertGreen fluorescent protein
ExpressionBacterialPromoterPclpBAvailable SinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only