30,756 results

1. pOPINK-OTUB1 (full-length, aa 1-271)

   Plasmid

   #61420

   Purpose

   Expresses human OTUB1 (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)
   

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. mSA-phi29

   Plasmid

   #210815

   Purpose

   in vitro transcription of mRNA

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

   Insert

   mSA-Phi29
   

   Expression

   Bacterial

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-SUMO1

   Plasmid

   #185637

   Purpose

   expression of GST-tagged SUMO1 in bacterial cells

   Depositor

   Irina Bezsonova

   Article

   Bregnard et al Biophys Chem. 2022 Aug;287:106827. doi:

   Insert

   small ubiquitin-like modifier 1 (SUMO1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET His6 MBP N10 TEV LIC cloning vector (2C-T)

   Plasmid

   #29706

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDF-mcherry1

   Plasmid

   #87144

   Purpose

   Constitutive E. coli mcherry Expression

   Depositor

   Michael Lynch

   Insert

   mcherry
   

   Expression

   Bacterial

   Promoter

   Constitutive proD

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFGL822

   Plasmid

   #58225

   Purpose

   For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.

   Depositor

   Naweed Naqvi

   Article

   Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

   Insert

   phosphinothricin acetyl transferase
   

   Expression

   Bacterial

   Promoter

   Aspergillus nidulans trpC promoter

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGRG37

   Plasmid

   #16667

   Depositor

   Nancy Craig

   Article

   McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

   Insert

   mTn7::Gateway cassette
   

   Expression

   Bacterial

   Available Since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-21a(+)-BMTU UDG-6xHis

   Plasmid

   #172197

   Purpose

   expresses His-tagged uracil-DNA-glycosylase enzyme from marine bacterium BMTU 3346 for the purpose of cross contamination prevention in nucleic acid amplification reactions

   Depositor

   Andrea Pauli

   Insert

   BMTU UDG
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLG008 (Ec78 retron)

   Plasmid

   #157886

   Purpose

   Expresses the Ec78 retron defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   Ec78 retron
   

   Expression

   Bacterial

   Available Since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET32-MCM2/7

    Plasmid

    #116949

    Purpose

    Expresses human MCM2 and human MCM7

    Depositor

    James Chong

    Article

    Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

    Inserts

    Human MCM7 (MCM7 Human)
    Human MCM2 (MCM2 Human)
    

    Tags

    Thioredoxin, His6

    Expression

    Bacterial

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTKEI-Dest

    Plasmid

    #79784

    Purpose

    Expression plasmid - BsmBI entry sites

    Depositor

    David Savage

    Article

    Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

    Type

    Empty backbone

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    pTrc

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSEVA421-Cas9tr

    Plasmid

    #138709

    Purpose

    Plasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenes

    Depositor

    Víctor de Lorenzo

    Article

    Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

    Insert

    tracrRNA-Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETSUMO-TtAgo

    Plasmid

    #210191

    Purpose

    Bacterial expression of the His- and SUMO-tagged Argonaute protein of Thermus thermophilus

    Depositor

    Phillip Zamore

    Article

    Smith et al Nat Commun. 2019 Jan 17;10(1):272. doi:

    Insert

    TtAgo (TT_P0026 Thermus thermophilus)
    

    Tags

    6x His tag and SUMO tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. iMbody_pSANG10-3F

    Plasmid

    #184496

    Purpose

    Expresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteria

    Depositor

    Marcel Dinger

    Article

    Zeraati et al STAR Protoc. 2023 Nov 21;4(4):102729. do

    Insert

    iMbody
    

    Tags

    3xFLAG, 6xHis, and pelB leader

    Expression

    Bacterial

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSR0

    Plasmid

    #161984

    Purpose

    Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection marker

    Depositor

    Olga Soutourina

    Article

    Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Oct. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTriEx-mCherry-LOV2 V416L

    Plasmid

    #81036

    Purpose

    mammalian expression of LOV V416L, a mutant with super long relaxation time

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV V416L
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    V416L

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW-LIC-N8C_Fab3b

    Plasmid

    #203685

    Purpose

    Expresses N8C_Fab3b

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab3b
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOTIII.EFGP.IGR3.6

    Plasmid

    #40866

    Depositor

    Harold Gainer

    Article

    Fields et al J Neurosci. 2003 Aug 27;23(21):7801-9.

    Insert

    MOUSE OXYTOCIN GENE (Oxt Mouse)
    

    Expression

    Bacterial

    Promoter

    mouse oxytocin promoter

    Available Since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. DYSF-3HA

    Plasmid

    #29767

    Depositor

    Steven Vogel

    Article

    Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

    Insert

    DYSF (DYSF Human)
    

    Tags

    3xHA

    Expression

    Bacterial and Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.3TOPO-T7-hCas9

    Plasmid

    #161876

    Purpose

    In vitro transcription of SpCas9

    Depositor

    Stephane Pelletier

    Article

    Pelletier et al Immunity. 2015 Jan 20;42(1):18-27. doi:

    Insert

    human codon optimized SpCas9
    

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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