30,756 results

1. pSJX024

   Plasmid

   #232327

   Purpose

   Genome editing in Caulobacter crescentus

   Depositor

   Wei Zhao

   Article

   Sun et al bioRxiv 2024.12.02.626314

   Inserts

   sgRNA
   SpCas9M
   

   Use

   CRISPR

   Tags

   H-arms and cat

   Expression

   Bacterial

   Promoter

   PJ23119 and Pxyl

   Available Since

   June 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22b_msfGFP_stag_KS

   Plasmid

   #237630

   Purpose

   For expression of msfGFP_stag_KS in Ecoli

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   stag_KS
   

   Tags

   msfGFP

   Expression

   Bacterial

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET22b_msfGFP_stag_KS(FtoG)

   Plasmid

   #237631

   Purpose

   For expression of msfGFP_stag_KS(FtoG) in Ecoli

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   stag_KS(FtoG)
   

   Tags

   msfGFP

   Expression

   Bacterial

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. ZIKV isolate PRVABC59 NS3 helicase domain

   Plasmid

   #228664

   Purpose

   Bacterial expression of the Zika virus NS3 helicase

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Zika virus NS3 helicase
   

   Tags

   His-GST-TEV

   Expression

   Bacterial

   Available Since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. RoL10X_pET29b

   Plasmid

   #215427

   Purpose

   Expression construct for RoL10X gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   RoL10X
   

   Expression

   Bacterial

   Mutation

   V209L/D262G/E190C/E238C/F173Y/V175F/G177C/Q197H/F…

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. ThcCut1_pET29b

   Plasmid

   #215429

   Purpose

   Expression construct for ThcCut1 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   ThcCut1
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. SPLICSFAST-ER-PM-P2A

   Plasmid

   #214893

   Purpose

   Expresses equimolar Plasma Membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PM Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-ER-PM-P2A
   

   Tags

   none

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJOE4847.2

   Plasmid

   #135086

   Purpose

   rhamnose-inducible expression vector

   Depositor

   Josef Altenbuchner

   Article

   Motejadded et al Biotechnol Lett. 2009 Apr;31(4):543-9. d

   Insert

   MalE-6xHis-Ulp1
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. NUDT3A

   Plasmid

   #42348

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   NUDT3A (NUDT3 Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pUCXMG

    Plasmid

    #204409

    Purpose

    Expression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI site

    Depositor

    David Ackerley

    Article

    Rich et al bioRxiv. 2023 Mar 25:2023.03.24.534183.

    Type

    Empty backbone

    Tags

    His6

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Capping Protein

    Plasmid

    #89950

    Purpose

    F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends.

    Depositor

    Antoine Jégou, Guillaume Romet-Lemonne

    Article

    Shekhar et al Nat Commun. 2015 Nov 13;6:8730. doi: 10.

    Inserts

    F-actin-capping protein subunit alpha-1 (Capza1 Mouse)
    F-actin-capping protein subunit beta (Capzb Mouse)
    

    Tags

    6*His

    Expression

    Bacterial

    Mutation

    V222I

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-SUMO-MjDim1

    Plasmid

    #189936

    Purpose

    Expression of Mjdim1, a methyltransferase used in m6A-SAC-seq.

    Depositor

    Chuan He

    Article

    Hu et al Nat Biotechnol. 2022 Mar 14. pii: 10.103

    Insert

    rsmA (MJ_RS00455 Methanocaldococcus jannaschii)
    

    Tags

    6x His tag, FLAG tag, SUMO tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. IRE-SunTag

    Plasmid

    #196920

    Purpose

    The 5UTR of the ferritin heavy chain mRNA was cloned upstream of the SunTag-Renilla coding sequence in the ΔIRE-SunTag reporter

    Depositor

    Jeffrey Chao

    Article

    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

    Insert

    Renilla luciferase
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKEW189-MBP-TEV-AcrVA1

    Plasmid

    #115660

    Purpose

    Expresses AcrVA1 in bacteria via T7 RNAP, for purification

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    AcrVA1
    

    Tags

    MBP-TEV

    Expression

    Bacterial

    Promoter

    T7 RNAP

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNBU2_erm_P1T_DP-GH023 - NanoLuc

    Plasmid

    #117727

    Purpose

    Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023 upstream of the NanoLuc reporter

    Depositor

    Andrew Goodman

    Article

    Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

    Insert

    NanoLuc
    

    Use

    Luciferase

    Expression

    Bacterial

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. K560GFP

    Plasmid

    #129761

    Purpose

    Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Tags

    GFP and His6

    Expression

    Bacterial

    Mutation

    Truncation at 559

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-His-MBP-NLS-RfxCas13d-NLS-HA

    Plasmid

    #171586

    Purpose

    Bacterial expression of NLS-RfxCas13d-NLS-HA

    Depositor

    Neville Sanjana

    Article

    Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

    Insert

    His-MBP-NLS-RfxCas13d-NLS-HA
    

    Tags

    HA

    Expression

    Bacterial

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. human p53-(1-320)

    Plasmid

    #24864

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 1-320

    Available Since

    Aug. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

19. KLHL12

    Plasmid

    #38908

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KLHL12 (KLHL12 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pET41a iGluu

    Plasmid

    #119834

    Purpose

    Production of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluu
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    S72T

    Promoter

    T7

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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