Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,163 results

Showing: 6571 - 6600 of 32163 results

  1. RHOAA-c035

    Plasmid
    #205118
    Purpose
    RHOA protein expression
    Depositor
    Paul Brennan
    Insert
    RHOA (RHOA Human)
    Tags
    6His-TEV and AviTag
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    205118-plasmid-map-sequence-id-411209

    Enlarge

  3. pET tdTomato LIC cloning vector (H6-tdTomato)

    Plasmid
    #86928
    Purpose
    pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    6xHis, AviTag (biotin), TEV cleavage site, and td…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    86928-plasmid-map-sequence-id-175058

    Enlarge

  5. pNAS1B

    Plasmid
    #61968
    Purpose
    Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors
    Depositor
    Lynne Regan
    Article
    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.
    Inserts
    mCherry
    GFP
    Expression
    Bacterial
    Mutation
    Serine 65 to Glycine (GGT), Serine 72 to Alanine …
    Promoter
    PBAD and PLtetO
    Available Since
    Jan. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    61968-plasmid-map-sequence-id-101267

    Enlarge

  10. pAC1430

    Plasmid
    #127981
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    127981-plasmid-map-sequence-id-254494

    Enlarge

  11. RRAS

    Plasmid
    #38815
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RRAS (Rras Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pSF18

    Plasmid
    #222593
    Purpose
    BB2 ZWF1 tet-on overexpression cassette
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    ZWF1
    Expression
    Bacterial
    Mutation
    Internal BsaI and BpiI sites removed from insert
    Promoter
    tet-on
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    222593-plasmid-map-sequence-id-443809

    Enlarge

  15. pTU2S-a (p15A origin)

    Plasmid
    #74088
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 2 TU's with secondary module and p15A origin
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    None
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74088-plasmid-map-sequence-id-138296

    Enlarge

  20. HECTD1

    Plasmid
    #25183
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    hectd1.1273.1343 (HECTD1 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  21. mEmerald-pBAD

    Plasmid
    #54220
    Purpose
    Localization: Protein Expression Vector , Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    6xHis and mEmerald
    Expression
    Bacterial
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. Dead Streptavidin-SpyCatcher

    Plasmid
    #59547
    Purpose
    pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead-dloop-Streptavidin-SpyCatcher
    Tags
    N-terminally shortened SpyCatcher
    Expression
    Bacterial
    Mutation
    PolyAsp in L3/4 loop for ion-exchange
    Available Since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  27. pST50Trc1-HISNDHFR

    Plasmid
    #63940
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis; TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63940-plasmid-map-sequence-id-177346

    Enlarge

  29. pBlu-CGT-1

    Plasmid
    #170158
    Purpose
    Expresses bacterial Cyclomaltodextrin Glucanotransferase from Paenibacillus sp. c36 in E.coli
    Depositor
    Sarah MacDonald
    Insert
    Cyclomaltodextrin Glucanotransferase
    Expression
    Bacterial
    Promoter
    Tac
    Available Since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    170158-plasmid-map-sequence-id-392677

    Enlarge

Showing: 6571 - 6600 of 32163 results