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Showing: 11151 - 11200 of 29646 results

  4. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79887
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79887-plasmid-map-sequence-id-402553

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  5. PDLIM5

    Plasmid
    #38811
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PDLIM5 (Pdlim5 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pWL502-crRNA

    Plasmid
    #174383
    Purpose
    The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei
    Depositor
    Jing Han
    Article
    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:
    Insert
    a crRNA targeting the template strand
    Use
    CRISPR; Archaeal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PhaR promoter
    Available since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    174383-plasmid-map-sequence-id-343840

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  7. ataxin3:1-431_Q79_BV

    Plasmid
    #209733
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    Ataxin 3 (ATXN3 Human)
    Use
    Tags
    6xHis-SBP-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Polyhedrin
    Available since
    Nov. 13, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    209733-plasmid-map-sequence-id-414202

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  12. pKD279

    Plasmid
    #125099
    Purpose
    Expression of SO_4388(1-130aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
    Depositor
    Jeffrey Tabor
    Article
    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d
    Inserts
    SO_4388(REC)-psdR(DBD)137
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    125099-plasmid-map-sequence-id-245241

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  13. DDX5A

    Plasmid
    #42421
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DDX5A (DDX5 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. pKCyR6

    Plasmid
    #149493
    Purpose
    Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Spectinomycin Resistance Marker.
    Depositor
    Christopher Reisch
    Article
    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629
    Insert
    CymR(AM)-mRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PCymRC
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    149493-plasmid-map-sequence-id-314927

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  15. pAV114

    Plasmid
    #63181
    Purpose
    pRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63181-plasmid-map-sequence-id-104885

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  18. SDC229-B03

    Plasmid
    #58763
    Purpose
    bacterial expression of PDB 4PYZ
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4PYZ (USP7 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available since
    Aug. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pOPINO

    Plasmid
    #41140
    Depositor
    Ray Owens
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    Available since
    March 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    41140-plasmid-map-sequence-id-54734

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  37. SIRT5

    Plasmid
    #25487
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SIRT5 (SIRT5 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25487-plasmid-map-sequence-id-203224

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  38. MACROD1

    Plasmid
    #39041
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MACROD1 (MACROD1 Human)
    Use
    Tags
    His6-Thioredoxin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  41. pSB4A3-PBAD-phzH

    Plasmid
    #165618
    Purpose
    Arabinose-inducible expression of phzH for conversion of phenazine-1-carboxylic acid to phanzine-1-carboxamide
    Depositor
    Martin Buck
    Insert
    phzH (phzH Pseudomonas aeruginosa)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    165618-plasmid-map-sequence-id-331404

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  42. pGBW-m4136463

    Plasmid
    #149527
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149527-plasmid-map-sequence-id-300219

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  43. pGBW-m4046764

    Plasmid
    #145673
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 27, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145673-plasmid-map-sequence-id-292487

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  46. JMJD2C

    Plasmid
    #39070
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    JMJD2C (KDM4C Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 11151 - 11200 of 29646 results