32,684 results
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Plasmid#177714PurposeCytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019DepositorInserthFluc WT
ExpressionBacterial and MammalianAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL1203
Plasmid#196024PurposeExpression/purification of 6xHis-TEV-MlaCDepositorInsertMlaC
ExpressionBacterialAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
R701-X22-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D
Plasmid#159571PurposeE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13DDepositorInsertHis6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D (KRAS Human)
TagsHis6-MBP-tev-AviExpressionBacterialMutationG13DAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFTK020
Plasmid#171292PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPalcA syn_NoCrea (ANIA_08979) promoter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM6-P.accABCD
Plasmid#66866PurposeEncodes accA, accB, accC, accD in Pseudo-operon configuration.DepositorInsertsaccA
accB
accC
accD
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pED14xS-1
Plasmid#134800PurposeIPTG inducible expression of S-TAGA qtRNADepositorInsertS-TAGA qtRNA
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pED14xS-WT
Plasmid#134804PurposeIPTG inducible expression of WT S tRNA scaffold tRNADepositorInsertWildtype S tRNA (decodes TCG)
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Orange Nano-lantern/pRSETB
Plasmid#67659Purposeorange luminescent protein for high-speed single-cell and whole body imagingDepositorInsertOrange Nano-lantern
Tags6xHisExpressionBacterialAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
cp193g7
Plasmid#25950DepositorInsertcp193g7
TagsHisExpressionBacterialAvailable SinceSept. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHAT4
Plasmid#112585PurposeUsed for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag fusionsDepositorTypeEmpty backboneTags6HisExpressionBacterialAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-NCBD + ACTR
Plasmid#99335PurposeBacterial coexpression of CBP NCBD and NCoA3 binding domainsDepositorExpressionBacterialPromoterT7Available SinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109AsAmp30E
Plasmid#123144PurposeArsenic sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL
Plasmid#119952PurposeExpresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.DepositorInsertsgpps
limS
ExpressionBacterialMutationN-terminal truncation of 56 amino acid residues (…Available SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDUET-StrepSUMO-DaPFOR
Plasmid#217819PurposeExpresses DaPFOR in E. coliDepositorInsertpACYCDUET-StrepSUMO-DaPFOR
TagsStrepSUMOExpressionBacterialPromoterT7Available SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pJ411 FUS 1-163 Y24xF
Plasmid#127196PurposeProtein expression for affinity purificationDepositorInsertFUS 1-163 Y24xF (FUS Human)
TagshexahistidineExpressionBacterialMutationY24xF: Y6F, Y14F, Y17F, Y25F, Y33F, Y38F, Y41F, Y…Available SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
RPRD1B
Plasmid#40739PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceNov. 14, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBbA5a-RFP
Plasmid#35280DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterlacUV5Available SinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBad-HisB-GdT
Plasmid#87361PurposeExpresses GdT, a dimeric green fluorescent proteinDepositorInsertGdT
TagsHis-TagExpressionBacterialPromoterAraBadAvailable SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCB-CBEv1
Plasmid#221138PurposeCoxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.DepositorInsert3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
UseCRISPRTags3xFLAGExpressionBacterialPromoterlacIq-PtacAvailable SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-yibDp-Erv1p
Plasmid#202483PurposeExpresses sulfydryl oxidase (Erv1p) after phosphate depletionDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-RGG-SmBit-RGG
Plasmid#176644PurposeSmBit fused to LAF-1 RGG domain with N-terminal MBP tagDepositorInsertMBP-RGG-SmBit-RGG
ExpressionBacterialAvailable SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
RAB30
Plasmid#25542PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 25, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
p045
Plasmid#70057PurposeCo-expresses Syt1-SNARE37aa-linker complex with pDC184DepositorInsertsC2AB-linker-SNAP-25_C
SNAP-25_N
TagsNo tagsExpressionBacterialPromoterT7Available SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSkunk3-BLA
Plasmid#61531PurposePhagemid expressing streptomycin/spectinomycin resistance geneDepositorInsertaadA
ExpressionBacterialPromoterlacIAvailable SinceDec. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_Ub
Plasmid#172134PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
Tags6xHis-TEVExpressionBacterialPromoterT7Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-SpCas9-HF1
Plasmid#126770PurposeExpression of increased fidelity SpCas9-HF1 in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 BAG3 WW domain
Plasmid#104266PurposeBacterial expression of WW domain from BAG3DepositorInsertBAG3 WW domain (BAG3 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH29_32 series plasmid
Plasmid#120901PurposeAlso known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated deviceDepositorInsertsEc-TTL-P109
sRNA-A targeting sequence
RBS TIR=974 of GFPop1 gene
GFPop1
RBS TIR=6474 of ECF32mut gene
ECF32mut
T7 terminator
resource competitor
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
PaBphP 1-497
Plasmid#36920DepositorInsertPaBphP-PCD
TagsHisExpressionBacterialMutationContains the photosensory core domain coding regi…PromoterT7Available SinceMay 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
TwinStrep-SUMO-Pnf
Plasmid#198321PurposeFor affinity purification of Pnf payloadDepositorInsertTwinStrep-SUMO-Pnf
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPSB2
Plasmid#38983PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
K401-Micro
Plasmid#122485PurposeComponent of optically-induced motor dimerization systemDepositorInsertHis tag MBP Kinesin 1-401 SspB micro
TagsH6-MBP-TEV and SspB microExpressionBacterialMutationKinesin amino acids 1-401Available SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
K401-iLid
Plasmid#122484PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-401 iLid His tag
TagsHis6 and iLidExpressionBacterialMutationKinesin amino acids 1-401PromoterT7Available SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
GUADA
Plasmid#42382PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNAND-PhlF
Plasmid#49376DepositorInsertPhlF repressor, pPhlF-pLmrA reporter
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSA3
Plasmid#52263PurposeBacterial expression of human 6His-tagged SUMO3 and SUMO-E1DepositorTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A2NT
Plasmid#62257Purposeexpression of A2NT sgRNA from the arabinose-inducible promoterDepositorInsertA2NT sgRNA
UseCRISPRExpressionBacterialPromoterpBADAvailable SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaB-mEGFPBb
Plasmid#118234PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mEGFPDepositorInsertflagellin promoter driven mEGFP
ExpressionBacterialAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_ZIKVC
Plasmid#145339PurposeCell-free/mammalian expression vector for Zika virus capsid protein with N-term eGFPDepositorInsertZIKV Capsid
Tags8xHis and eGFPExpressionBacterialPromoterT7- CMV/CAGAvailable SinceApril 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFVTd5
Plasmid#167520PurposeBroad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, tdKatushka2, Gentamicin Resistance MarkerDepositorInsertVanRAM-tdKatushka2
UseSynthetic BiologyExpressionBacterialPromoterPVanCCAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSV272-PfMBP-HypDAE
Plasmid#110296PurposeExpresses t-4-hydroxy-L-proline dehydratase activating enzyme in E. coli BL21(DE3)DepositorInsertt-4-Hydroxy-L-proline Dehydratase Activating Enzyme
TagsHis6 and Maltose Binding ProteinExpressionBacterialPromoterT7Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Xenopus (Silurana) tropicalis
Plasmid#75355PurposeNt His-tag Xenopus (Silurana) tropicalis Sacsin 87-1376DepositorInsertSacsin Xenopus (Silurana) tropicalis 87-1376
TagsHis-TagExpressionBacterialAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(WT)
Plasmid#111323Purposefor T7 transcription of wt actin pre-mRNADepositorInsertACT1
ExpressionBacterialAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD-SYNZIP4
Plasmid#80660PurposeEncodes the synthetic coiled coil peptide SYNZIP4DepositorInsertSYNZIP4
Tags6xHisExpressionBacterialAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL001
Plasmid#115674PurposeFluorescent reporter for E.coli K12 fliC promoter activityDepositorInsertfliC promoter fused with GFP
TagsGreen fluorescent protein (GFP)ExpressionBacterialPromoterfliC promoterAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT2
Plasmid#53558Purposebacterial expression of IFIT2DepositorAvailable SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
TOPO PuroR
Plasmid#68447PurposeTOPO construct expressing PuroR for generation of GMAP-compatible geneDepositorInsertPuromycin Resistance
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMflET-o4
Plasmid#101314PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
ereB resistance gene
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQMD
Plasmid#48103PurposeDestination plasmid (Gateway cloning) for cumate-inducible expression of myc-tagged fusion proteinDepositorTypeEmpty backboneTagsmyc tagExpressionBacterialPromoterPQ5Available SinceJan. 28, 2014AvailabilityAcademic Institutions and Nonprofits only