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31,059 results

Showing: 9081 - 9120 of 31059 results

  4. rpoS200-RFP

    Plasmid
    #63840
    Purpose
    This plasmid is designed as a bacterial stationary phase sensor
    Depositor
    Howard Salis
    Insert
    rpoS, mRFP1 and GFPmut3b
    Tags
    mRFP1
    Expression
    Bacterial
    Mutation
    Gene is translationally fused to RFP
    Available Since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    63840-plasmid-map-sequence-id-106563

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  8. pTH385-GST-TEV-SUP35

    Plasmid
    #29744
    Depositor
    Tobias von der Haar
    Insert
    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    wild type
    Available Since
    Aug. 8, 2011
    Availability
    Academic Institutions and Nonprofits only
    29744-plasmid-map-sequence-id-13828

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  10. pGBW-m4046754

    Plasmid
    #145647
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145647-plasmid-map-sequence-id-292580

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  11. G-SMARCA4-2

    Plasmid
    #166519
    Purpose
    scFv of a human scaffold targeting SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a4 isoform B. Antigen coverage aa 1451-1580 of 1647, Bromodomain.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166519-plasmid-map-sequence-id-329058

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  12. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

    Plasmid
    #61966
    Purpose
    Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))
    Depositor
    Lynne Regan
    Article
    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.
    Inserts
    N-mCherry-ME(pS)VD
    TRAP(RK)-C-mCherry
    Expression
    Bacterial
    Promoter
    PBAD and PLtetO
    Available Since
    Feb. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61966-plasmid-map-sequence-id-101265

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  15. pHS1543

    Plasmid
    #205963
    Purpose
    NZ_CP025815 DinG HNH Cascade proteins operon (E. coli codon optimized) with His14-tagged Csf3 in pET45b(+)
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Insert
    DinG HNH Cascade with His14-tagged Csf3
    Tags
    14xHis on Csf3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205963-plasmid-map-sequence-id-415370

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  20. p2B-57

    Plasmid
    #177927
    Purpose
    Expresses yeGFP under control of doxycycline-responsive TET3 promoter (contains three rtTA binding sites)
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    EGFP
    Expression
    Bacterial and Yeast
    Promoter
    TET3 (contains three rtTA binding sites)
    Available Since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177927-plasmid-map-sequence-id-349993

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  21. Clover-pBAD

    Plasmid
    #54575
    Purpose
    Localization: Protein Expression Vector , Excitation: 505, Emission: 515. This plasmid encodes Clover H231L based on reference from Bajar et. al. Sci Rep. 2016.
    Depositor
    Michael Davidson
    Article
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Type
    Empty backbone
    Tags
    6xHis and Clover
    Expression
    Bacterial
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. MSP2262

    Plasmid
    #70703
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A & H557A in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70703-plasmid-map-sequence-id-127765

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  35. roGFP_iE_C147S_pQE30

    Plasmid
    #48631
    Purpose
    Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 147 to Serine mutation (roGFP C147S, E147b, C204)
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    roGFP1
    Tags
    HisTag
    Expression
    Bacterial
    Mutation
    insertion E147B, C147S
    Available Since
    Oct. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    48631-plasmid-map-sequence-id-70379

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Showing: 9081 - 9120 of 31059 results