30,803 results

1. pEGFP_PCNA_IRES_puro2b

   Plasmid

   #21048

   Depositor

   Daniel Gerlich

   Article

   Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

   Insert

   hPCNA
   

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Available Since

   May 15, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. DYRK1A

   Plasmid

   #38913

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   DYRK1A (DYRK1A Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   codon-optimized

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. PA-Cdc42

   Plasmid

   #75263

   Purpose

   Light-activated Cdc42

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   Cell Division Cycle 42 (CDC42 Human)
   

   Tags

   6xHIs, LOV2, and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   F56W

   Promoter

   CMV

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRAU168

   Plasmid

   #86834

   Purpose

   bacterial expression of anti-CRISPR protein AcrIIA1

   Depositor

   Joseph Bondy-Denomy

   Article

   Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

   Insert

   acrIIA1
   

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJMP1183

   Plasmid

   #119254

   Purpose

   rfp "test" strain

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNCS tdTomato

   Plasmid

   #91767

   Purpose

   Bacterial expression of a fluorescent protein, tandem dimer Tomato (tdTomato)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Insert

   tandem dimer Tomato
   

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBbE2a-RFP

   Plasmid

   #35322

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Tet

   Available Since

   Feb. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. p15A-OptoCre-knt-P-R*

   Plasmid

   #189852

   Purpose

   Cre-inducible activation of knt for kanamycin resistance.

   Depositor

   Mary Dunlop

   Article

   Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

   Insert

   P-loxP-TT-loxP-R*-knt
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P (T7 phage gene 10)

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSETB PKA RIalpha

   Plasmid

   #14922

   Depositor

   Susan Taylor

   Article

   Taylor et al Biochim Biophys Acta. 2005 Dec 30. 1754(

   Insert

   PKA R1alpha regulatory subunit (PRKAR1A Bovine)
   

   Expression

   Bacterial

   Available Since

   July 24, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGDP1 vph

    Plasmid

    #112893

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    vph
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    Pbla

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET22b-TEVP-cleavableELP

    Plasmid

    #219402

    Purpose

    Tobacco etch virus protease-responsive elastin-like polypeptide (ELP)

    Depositor

    Danielle Mai

    Article

    Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

    Insert

    TEVP-cleavableELP
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGDP3 vatD

    Plasmid

    #112885

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    vatD
    

    Expression

    Bacterial

    Promoter

    Pbla

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpyLigase

    Plasmid

    #51722

    Purpose

    Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!

    Depositor

    Mark Howarth

    Article

    Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

    Insert

    SpyLigase
    

    Tags

    His6 Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJBEI-6411

    Plasmid

    #47050

    Purpose

    BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
    

    Use

    Synthetic Biology; Bglbrick

    Expression

    Bacterial

    Promoter

    Pbad

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115220

    Purpose

    Expresses Pin3Cas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    Pin3Cas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTKEI-Mal-B2

    Plasmid

    #79756

    Purpose

    Single fluorescent protein biosensor for maltose

    Depositor

    David Savage

    Article

    Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

    Insert

    Mal-B2
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    pTrc

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

    Plasmid

    #101218

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N692A/M694A/Q695A/H698A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A and H698A

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. His-MBP human SPOP (aa 28-337)

    Plasmid

    #52294

    Purpose

    bacterial expression of human SPOP (aa 28-337) fused to His-MBP

    Depositor

    Brenda Schulman

    Article

    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

    Insert

    SPOP (SPOP Human)
    

    Tags

    His-MBP and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115210

    Purpose

    Expresses AspCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    AspCas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-IMX-DogTag

    Plasmid

    #113765

    Purpose

    Expresses IMX-DogTag in bacterial cells for modular antigen multimerization using SnoopLigase

    Depositor

    Mark Howarth

    Article

    Andersson et al Sci Rep. 2019 Mar 15;9(1):4625. doi: 10.

    Insert

    IMX-DogTag
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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