29,776 results

1. pRSFDuet-1_VNp6-DARPinOFF7

   Plasmid

   #182398

   Purpose

   Bacterial expression of Vesicle Nucleating peptide6-DARPinOFF7 fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp6-DARPinOFF7
   

   Use

   Tags

   Vesicle Nucleating peptide 6 (VNp6)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKFSS1_2

   Plasmid

   #118227

   Purpose

   Empty E. coli - B. burgdorferi shuttle vector; streptomycin resistant

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQexp

   Plasmid

   #25820

   Depositor

   George Church

   Article

   Tolonen et al Mol Microbiol. 2009 Dec . 74(6):1300-13.

   Insert

   pQexp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAG4

   Plasmid

   #70115

   Purpose

   Tn7-based lux reporter for promoter cloning in bacteria

   Depositor

   Thomas Lewis

   Article

   Glassing et al J Microbiol Methods. 2015 Sep 1;118:75-7

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pET11a_PTK2b_Kinase

   Plasmid

   #224242

   Purpose

   Expression of human PTK2b Kinase domain in E. coli

   Depositor

   Andrew Mesecar

   Insert

   ptk2b_kinase (PTK2B Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDFDuet-MKK6-EE

   Plasmid

   #82718

   Purpose

   Expresses constitutively active human MKK6. Multiple cloning site 2 is empty.

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Ser207 to Glu, Thr211 to Glu

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

7. CYP2C9 in vector pCW ori+

   Plasmid

   #69554

   Purpose

   Modification of CYP2C9 and 2C19 s for bacterial expression

   Depositor

   Joyce Goldstein

   Insert

   CYP2C9 (CYP2C9 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRE-Tn7-146

   Plasmid

   #118562

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mCardinal
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKD184

   Plasmid

   #90953

   Purpose

   Expresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutively

   Depositor

   Jeffrey Tabor

   Article

   Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

   Inserts

   Thiosulfate response regulator
   Superfolder GFP
   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Bba_J23114, PltetO-1, and PphsA

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHCKan-yibDp-GFPuv

    Plasmid

    #127078

    Purpose

    Low phosphate inducible yibD gene promoter expressing GFPuv reporter

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMRE-Tn5-140

    Plasmid

    #118524

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Use

    Minitn5 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAMK2G

    Plasmid

    #38894

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CAMK2G (CAMK2G Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pRMC2-tat:msfGFP

    Plasmid

    #194915

    Purpose

    Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. RAB1A

    Plasmid

    #25521

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB1A (RAB1A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pRIM3-rfp

    Plasmid

    #74073

    Purpose

    GentR version of pRIM2 carrying rfp

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    rfp
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 3xFLAG-CUL4A-pCMV7.1

    Plasmid

    #155022

    Purpose

    N-terminal 3xFLAG-tagged CUL4A in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 4A (CUL4A Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pCMV7.1

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDSG594

    Plasmid

    #214221

    Purpose

    competition strain 10 integrase plasmid

    Depositor

    Uri Alon

    Article

    Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

    Insert

    PhlF, A118
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDUAL_2_araBAD

    Plasmid

    #155176

    Purpose

    see pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-X(4130)B1

    Plasmid

    #168018

    Purpose

    Expresses X(4130)B1-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    X(4130)B1-6xHis
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX 4T1 hBad

    Plasmid

    #35567

    Depositor

    Harish Srinivas

    Article

    Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

    Insert

    bad (BAD Human)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only