34,231 results
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Plasmid#90954PurposeExpresses S. baltica TtrS under the Ptac promoterDepositorInsertTetrathionate sensor kinase
ExpressionBacterialAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSR83
Plasmid#90211PurposeExpresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoterDepositorAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-BLa 1.2
Plasmid#89000PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.DepositorInsertC-BLa 1.2 fusion protein
TagsFlagExpressionBacterialPromoteraraBADAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCasY1
Plasmid#87687Purposep15A plasmid expressing CasY1DepositorInsertCasY1
ExpressionBacterialAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET14-GFP30_Encap
Plasmid#86406PurposeExpresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 30aa CLP tag
Encapsulin
Tags6x HistidineExpressionBacterialPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMyBADNT
Plasmid#84690PurposeProtein expression in mycobacteriaDepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterAraCAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET mRuby2 LIC cloning vector (H6-mRuby2)
Plasmid#86934PurposepET LIC cloning vector for Biotin-His6-tev-yORF-mRuby2DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), TEV cleavage site, and mR…ExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ana GvpC-SpyTag-His in pET28a
Plasmid#85737PurposeExpresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coliDepositorInsertGas vesicle protein C (fused to SpyTag and His-tagged)
TagsHis-tag and SpyTagExpressionBacterialMutationAdded an extra glycine after the methionine at th…PromoterT7Available sinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYGD
Plasmid#80755PurposeExpression of human gamma D-crystallin in E. coliDepositorAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTriEX-Antenna-GDI Rac1
Plasmid#83359PurposeExpression of Antenna-Rac1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRac1 (RAC1 Human)
TagsFRET Antenna (mCerulean-cpVenus) and HisExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable sinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_Trigger_32B
Plasmid#75009PurposePortion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequenceDepositorInsertZIKV_Trigger_32B
ExpressionBacterialPromoterT7Available sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRT7 NT Topo arabinose
Plasmid#73446PurposeUsed for arabinose-induced expression of N-term GST/C-term 6xHis tagged peptidesDepositorTypeEmpty backboneTagsGST (N-terminal), 6xHis (C-terminal)ExpressionBacterialPromoterpBadAvailable sinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Ubiquitin G76C
Plasmid#86583PurposeBacterial expression of Ubiquitin G76C for cross linking studiesDepositorInsertUbiquitin (UBC Human)
TagsHis6-MBPExpressionBacterialMutationTEV site; G76CPromoterTacAvailable sinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZIKV_Trigger_27B
Plasmid#75008PurposePortion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequenceDepositorInsertZIKV_Trigger_27B
ExpressionBacterialPromoterT7Available sinceFeb. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWUR 1+2 tetO mut89
Plasmid#80102PurposeEncodes a set of wild-type Cas1+2 and a mutant set of Cas1+2DepositorPublicationExpressionBacterialMutationQ24H, P202Q, G241D, E276D, L297QAvailable sinceDec. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCis mCherry-SS Himar1 A7
Plasmid#84687PurposeCan be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.DepositorTypeEmpty backboneExpressionBacterialAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R472A_H477A
Plasmid#83483PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).DepositorInsertLbu_C2c2_R472A_H477A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR472A, H477AAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNCS-CyRFP1
Plasmid#84546PurposeBacterial expression of CyRFP1DepositorInsertCyRFP1
TagsHisExpressionBacterialAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSKA413
Plasmid#80381PurposeConstitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.DepositorInsertsccaS (complement)
ccaR (complement)
gfpmut3
UseSynthetic Biology; OptogeneticsTagsFLAG tagExpressionBacterialPromoterccaR and cpcG2Δ59Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHLUK
Plasmid#64181Purposeharbours marker genes HIS3, LEU2, URA3 and LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by