30,261 results

1. pTarget12f1

   Plasmid

   #171183

   Purpose

   Constitutive expression of sgRNA without donor DNA

   Depositor

   Kohsuke Honda

   Article

   Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

   Insert

   sgRNA scaffold in the CRISPR/Cas12f1 system
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119(SpeI) promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHEA

   Plasmid

   #168297

   Purpose

   Bacterial surface display of polypeptides (nanobodies) in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.

   Insert

   Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
   

   Use

   Tags

   6xHis, E-tag, and PelB signal peptide

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD/HisB-PAmKate

   Plasmid

   #32691

   Depositor

   Vladislav Verkhusha

   Article

   Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

   Insert

   PAmKate
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   F84W/S148N/S165G compared to mKate (but numbering…

   Promoter

   araB

   Available since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. WT USP7 TRAF

   Plasmid

   #227226

   Purpose

   Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Article

   Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   aa 62-205

   Promoter

   T7-promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PAS528 (TJF059)

   Plasmid

   #65602

   Purpose

   pETDuet-1 Containing FadD-His

   Depositor

   Pamela Silver

   Article

   Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

   Insert

   FadD
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lacO

   Available since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pUC57-HBB-nLuc

   Plasmid

   #175432

   Purpose

   Used to generate IVT RNA for translation extracts

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   HBB-nLuc
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHis-LMW_FGF2

   Plasmid

   #157657

   Purpose

   Expresses FGF2 in E.coli cell

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Fibroblast growth factor 2 (FGF2 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   changed Cystein 211,229 to serine, deleted amino …

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSG 25

   Plasmid

   #63867

   Purpose

   Expression of beta-galactosidase at high levels in E. coli

   Depositor

   Albert Dahlberg

   Article

   O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

   Insert

   lacZ (lacZ Escherichia coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PtacI

   Available since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

9. P70a-deGFP-sc101-kan

   Plasmid

   #184841

   Purpose

   Expression of deGFP

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   green fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   promoter OR2-OR1

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLC-237

    Plasmid

    #73191

    Purpose

    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (relE) under the control of arabinose induceable promoter (ParaB) for negative selection.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    ParaB-relE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ParaB

    Available since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDual-eGFP(Y203)

    Plasmid

    #63559

    Purpose

    Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP(Y203)
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Changed threonine at position 203 with respect to…

    Promoter

    CMV-EF1α hybrid (CEF)

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pTcI42-Wasabi

    Plasmid

    #86118

    Purpose

    mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TcI42
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T, K6N, S33T, Y61H, L119P, F122C

    Promoter

    pR/pL and pR/pL, LacI

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOSV809

    Plasmid

    #126602

    Purpose

    modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

    Depositor

    Jean-Luc Pernodet

    Article

    Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAF

    Plasmid

    #104152

    Purpose

    Plasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.

    Depositor

    Robert Craigie

    Article

    Lee et al Proc Natl Acad Sci U S A. 1998 Feb 17;95

    Insert

    Barrier-to-autointegration factor (BAF) (BANF1 Human)
    

    Use

    Tags

    N-terminal His tag with thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMMB66EH-aphA

    Plasmid

    #44392

    Depositor

    Victor DiRita

    Article

    Anthouard et al MBio. 2013 Aug 6;4(4). pii: e00403-13. d

    Insert

    AphA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZE31ms2

    Plasmid

    #25860

    Depositor

    Philippe Cluzel

    Article

    Le et al Proc Natl Acad Sci U S A. 2005 Jun 28. 1

    Insert

    ms2 binding site (x2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Nov. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBP-C_NanoLuc

    Plasmid

    #204112

    Purpose

    Type 0 CDS part

    Depositor

    Mikhail Shapiro

    Insert

    NanoLuc luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW-LIC-N8C_Fab4a

    Plasmid

    #203690

    Purpose

    Expresses N8C_Fab4a

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab4a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAUX_OL

    Plasmid

    #166246

    Purpose

    use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

    Depositor

    Domitilla Del Vecchio

    Article

    Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

    Insert

    [P112-sgRNA-term]-[J23116_B34-dCas9-B15]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ec-TTL-P112 and BBa_J23116

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBA615

    Plasmid

    #186239

    Purpose

    eLbuCas13a Negative Control (RFP targeting)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    pTet-LbuCas13a + crRNA negative control
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge