Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

Your Selections

Bacterial Expression

30,807 results

Showing: 5261 - 5280 of 30807 results

  1. pJB02

    Plasmid
    #111959
    Purpose
    Expresses GST-GFP-DHFR; construct generated for pull-down and localization experiments
    Depositor
    Matthew Good
    Article
    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259
    Inserts
    mEGFP
    eDHFR
    Tags
    DHFR
    Expression
    Bacterial
    Mutation
    eDHFR;residues 2-159 and enhanced GFP with monome…
    Promoter
    T7
    Available Since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111959-plasmid-map-sequence-id-219913

    Enlarge

  2. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid
    #193315
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    Tags
    4FLAG
    Expression
    Bacterial
    Available Since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    193315-plasmid-map-sequence-id-382367

    Enlarge

  4. pCFJ1415

    Plasmid
    #84828
    Purpose
    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 introns
    Depositor
    Andrew Fire
    Article
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized GFP
    Expression
    Bacterial and Worm
    Promoter
    Peft-3
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84828-plasmid-map-sequence-id-163670

    Enlarge

  6. pMAL-Abl-PRM 4R

    Plasmid
    #112087
    Purpose
    Bacterial expression plasmid containing His and MBP tags for 4 PRM motif repeats of Human Abl.
    Depositor
    Michael Rosen
    Article
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Insert
    Abl-PRM-4R (ABL1 Human)
    Tags
    His-6 and MBP
    Expression
    Bacterial
    Mutation
    construct contains only the PRM motif of Abl1
    Promoter
    Lac
    Available Since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112087-plasmid-map-sequence-id-236084

    Enlarge

  14. pNtCp-rbcL-PHLS-GPPS-aadA-accD

    Plasmid
    #52471
    Purpose
    Inserts the beta-phellandrene synthase gene, the geranyl diphosphate synthase gene, and aadA resistance in the rbcL-accD intergenic region in Nicociana tabacum
    Depositor
    Anastasios Melis
    Insert
    rbcL-PHLS-GPPS-aadA-accD
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52471-plasmid-map-sequence-id-81334

    Enlarge

  15. CYP2C8

    Plasmid
    #69604
    Purpose
    CYP2C8 cDNA IN PCw ORI+
    Depositor
    Joyce Goldstein
    Insert
    CYP2C8 (CYP2C8 Human)
    Expression
    Bacterial
    Available Since
    Oct. 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. EIF5A

    Plasmid
    #25260
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    eIF5A:S15-A151 (EIF5A Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids S15-A151
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 5261 - 5280 of 30807 results