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32,161 results

Showing: 10681 - 10720 of 32161 results

  1. HAO1

    Plasmid
    #38970
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HAO1 (HAO1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38970-plasmid-map-sequence-id-471362

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  4. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid
    #212122
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Tags
    His6 and SUMO
    Expression
    Bacterial
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212122-plasmid-map-sequence-id-444187

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  5. pALS3-Ma-SUMO-TAG35-sfGFP

    Plasmid
    #212120
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-TAG35-sfGFP
    M. alvus Pyl-tRNA
    Tags
    His6 and SUMO-TAG35
    Expression
    Bacterial
    Mutation
    E35TAG (within SUMO encoding sequence)
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212120-plasmid-map-sequence-id-444184

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  9. pAV123

    Plasmid
    #63189
    Purpose
    pRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63189-plasmid-map-sequence-id-331731

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  15. SIRT6

    Plasmid
    #41565
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SIRT6 (SIRT6 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    aa 3-353
    Promoter
    T7
    Available Since
    April 9, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. K560GFP_17NL

    Plasmid
    #129767
    Purpose
    Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    DAL insert before A345
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129767-plasmid-map-sequence-id-263589

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  32. USP21 (3MTN)

    Plasmid
    #28112
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP21 (USP21 Human)
    Tags
    His tag with Thrombin cleavage
    Expression
    Bacterial
    Mutation
    catalytic domain only (amino acid residues 209-56…
    Available Since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. pTXB1-H2B(1-114)_wt

    Plasmid
    #118252
    Purpose
    Bacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa
    Depositor
    Cynthia Wolberger
    Article
    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do
    Insert
    xlH2B(1-114)
    Tags
    Mxe-GyrA intein fused to chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    118252-plasmid-map-sequence-id-226720

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  36. pAH18

    Plasmid
    #154397
    Purpose
    Derived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    Fused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    NA
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    154397-plasmid-map-sequence-id-308811

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  39. pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

    Plasmid
    #101230
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…
    Promoter
    T7
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101230-plasmid-map-sequence-id-198024

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Showing: 10681 - 10720 of 32161 results