33,902 results
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Plasmid#162667PurposepET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
TagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCas3cRh
Plasmid#133773PurposeExpresses all necessary components of Type I-C CRISPR-Cas systemDepositorAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAS9counter
Plasmid#107192PurposeExpresses CAS9 and sgRNA for targeted counterselection in S. aureusDepositorInsertsCas9
sgRNA
ExpressionBacterialPromoterprab17Available sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET264-pUC 24xMS2V6 Loxp KANr Loxp
Plasmid#104393PurposeYeast endogenous mRNA taggingDepositorInsert24xMS2V6
ExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TDP-43_CTD
Plasmid#98669PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43DepositorAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNCS BFP
Plasmid#91757PurposeBacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)DepositorInsertBlue Fluorescent Protein
TagsHexa-Histidine, Xpress epitope for detection with…ExpressionBacterialMutationGFP (Y66H)PromoterT7Available sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSR58.6
Plasmid#63176PurposeExpresses CcaR constitutively and sfGFP under the PcpcG2-172 promoterDepositorInsertsCcaR
sfGFP
UseSynthetic BiologyExpressionBacterialAvailable sinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD/His-B-iRFP
Plasmid#31855Purposea phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imagingDepositorInsertiRFP
ExpressionBacterialMutationS13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D2…PromoteraraBADAvailable sinceSept. 16, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTriEx-mVenus-PA-Rac1
Plasmid#22007PurposeExpression of photoactivatable Rac1DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
Tags6X His and mVenusExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546), Rac1 starts at I4 and contain…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDest-544
Plasmid#11519DepositorTypeEmpty backboneUseGateway CloningTagsHis6-NusAExpressionBacterialAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET21a(+)-Bst LF-6xHis
Plasmid#159148Purposeexpresses His-tagged large fragment of Bst polymeraseDepositorInsertlarge fragment of Geobacillus stearothermophilus DNA polymerase I
TagsHisExpressionBacterialAvailable sinceSept. 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pTNS3
Plasmid#63127PurposeTn7 transposase expressionDepositorTypeEmpty backboneExpressionBacterialPromoterP1 and PtacAvailable sinceMay 17, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-tag-N
Plasmid#108078PurposeGateway compatible destination vectors containing StrepIII/HA/BioIDDepositorAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Pet28a_Human_H4
Plasmid#42633DepositorAvailable sinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
hGFAP-GFP
Plasmid#40592DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP-S65T
UseMouse TargetingTagsMP-1 fragment (mouse protamine 1 intron and polya…ExpressionBacterial and MammalianMutationConstruct contains a mutated, humanized hGFP(S65T…PromoterhGFAP promoterAvailable sinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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VHL-pGex2TK
Plasmid#20790DepositorPublicationAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-5FLAG-KanMX6
Plasmid#15983DepositorTypeEmpty backboneTags5xFlagExpressionBacterialAvailable sinceOct. 31, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQLinkG2
Plasmid#13671DepositorInsertmulti cloning site
UseCo-expressionTagsGST, TEV, and attB1ExpressionBacterialAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSFDuet-1_VNp15-mNeongreen
Plasmid#182389PurposeBacterial expression of Vesicle Nucleating peptide15-mNeongreen fusionDepositorInsertVNp15-mNeongreen-His
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by