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Plasmid#218960PurposeExpresses Hisx6-Tagged msYFP in bacteriaDepositorInsertmsYFP
UseTags6X-HisExpressionBacterialMutationPromoterT5Available sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
PJEBAN3
Plasmid#203682PurposeExpresses Yellow Fluorescent Protein (YFP) in Listeria monocytogenesDepositorInsertYellow Fluorescent Protein (YFP)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-sfGFP 150TAG
Plasmid#85493PurposepET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
UseTagsExpressionBacterialMutation150 TAG (amino acid 150 mutated to stop codon)PromoterT7Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
spNW25
Plasmid#173484PurposeExpresses spNW25 in Bl21DepositorInsertspNW25
UseTagsHistagExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCP30T-E2Crimson
Plasmid#78478PurposeBroad range bacterial fluorescence labelingDepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pComb3XSS
Plasmid#63890Purpose3rd generation plasmid for phage display on modified geneIII, contains stuffer fragmentDepositorInsertsSS Stuffer
Light Chain Stuffer
Heavy Chain Stuffer
UsePhage displayTags6x His, HA tag, and geneIIIExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC_pThrRS_SeptRNAv2.0CUA_OXB20-PduX_EFSep
Plasmid#173899PurposeThe pThr/tRNAv2.0CUA pair enables the biosynthesis of proteins containing pThrDepositorInsertsThrRS(v1.0)
tRNA(v2.0)CUA
pduX
EF-Sep
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSAM_Ec
Plasmid#102939PurposeTransposon mutagenesis and Tn-seqDepositorInsertmariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET32a-HD46Q
Plasmid#11515DepositorInserthuntingtin, exon 1, 46Q glutamines (HTT Human)
UseTagsHis, His (out of frame), and TrxExpressionBacterialMutationhuntingtin exon 1 with 46 glutamines, vector m…PromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCryptDel4.8
Plasmid#141293PurposePlasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.DepositorInsertsRelB
gRNA targetting pMUT2
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterNative promoter from pMUT2 relB/relE operon and P…Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET His6 TEV LIC cloning vector (1B)
Plasmid#29653DepositorTypeEmpty backboneUseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
PCV-Cas9
Plasmid#123643PurposeExpresses Cas9 with an N-terminal HUH-tag called PCV2DepositorInsertsUseTags(EAAAK)8 linker and His6 + SUMOExpressionBacterialMutation2-116PromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IF-GFP-ATRX
Plasmid#45444PurposeATRX expression vectorDepositorInsertATRX (ATRX Mouse, Human)
UseTagsGFP and HAExpressionBacterial and MammalianMutationisoform 2, missing codon E124PromoterPCMVAvailable sinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_His-TEV-EGFP
Plasmid#223723PurposeExpression of recombinant protein for purificationDepositorInsertEGFP, not monomeric (has A207)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET mCherry LIC cloning vector (u-mCherry)
Plasmid#29769DepositorTypeEmpty backboneUseTagsmCherryExpressionBacterialMutationPromoterT7Available sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pylT-N346A/C348A
Plasmid#127411PurposeDouble mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethersDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
UseTagsExpressionBacterialMutationN346A-C348APromoteraraBADAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBBR-Cas9ΔyqhD-HA500
Plasmid#220369PurposeA single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440DepositorInsertsSpCas9
sgRNA for yqhD in P. putida KT2440
UseCRISPR; Broad host rangeTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)
Plasmid#29725DepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), SiriusGFP, and TEV cleava…ExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBTK503
Plasmid#110616PurposeBTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoterDepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-mTurquoise2
Plasmid#108235PurposeBroad host bacterial expression vector with constitutive Pc promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.DepositorInsertPc promoter - mTurquoise2
UseTagsExpressionBacterialMutationcodon optimized for experession in P. fluorescensPromoterAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only