Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,457 results

Showing: 561 - 580 of 32457 results

  1. pet30b-7M SrtA

    Plasmid
    #51141
    Purpose
    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity
    Depositor
    Hidde Ploegh
    Insert
    S.aureus SrtA 7M
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    P94R, E105K, E108Q, D160N, D165A, K190E, K196T
    Promoter
    T7
    Available Since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    51141-plasmid-map-sequence-id-211598

    Enlarge

  2. HA-HA-AR-NTD

    Plasmid
    #171235
    Purpose
    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain (NTD)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    2xHA-tagged human androgen receptor trancate: N-terminal-domain (AR Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    AR truncate
    Promoter
    CMV
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171235-plasmid-map-sequence-id-336028

    Enlarge

  7. pThermoCas9i_ctrl

    Plasmid
    #100982
    Purpose
    Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module
    Depositor
    John van der Oost
    Article
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Tags
    None
    Expression
    Bacterial
    Mutation
    changed aspartate 8 to alanine and histidine 582 …
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available Since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100982-plasmid-map-sequence-id-392326

    Enlarge

  8. HA-HA-AR-DBD

    Plasmid
    #171238
    Purpose
    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain (DBD)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    2xHA-tagged human androgen receptor trancate:DNA-binding domain (AR Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    AR truncate
    Promoter
    CMV
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171238-plasmid-map-sequence-id-336038

    Enlarge

  9. pET-pA-Hia5

    Plasmid
    #174372
    Purpose
    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein A - hia5
    Tags
    6xHis and Protein A
    Expression
    Bacterial
    Available Since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174372-plasmid-map-sequence-id-341860

    Enlarge

  13. pMBP-hLIF

    Plasmid
    #179711
    Purpose
    Expresses H6-MBP-LIF
    Depositor
    Mark Arbing
    Insert
    Leukemia inhibitory factor (LIF Human)
    Tags
    His6-MBP-TEV protease site
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    179711-plasmid-map-sequence-id-352198

    Enlarge

  15. pEVOL_PylRS(AF)-tRNA

    Plasmid
    #223511
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-
    Inserts
    Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Y306A; Y384F
    Promoter
    araBAD, rrnB, and glnS and proK
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223511-plasmid-map-sequence-id-447439

    Enlarge

Showing: 561 - 580 of 32457 results