30,249 results

1. pJS65

   Plasmid

   #207587

   Purpose

   GFP-TonB expression

   Depositor

   Colin Kleanthous

   Article

   Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

   Insert

   GFP-TonB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ptrc99a-GPPS-CSstr-ispA*[JBEI-15060]

   Plasmid

   #100962

   Purpose

   Contains genes encoding GPP synthase, cineole synthase and IspA(S81F)

   Depositor

   Taek Soon Lee

   Insert

   GPPS-CSstr-ispA*
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPagP

   Plasmid

   #112125

   Purpose

   Bacterial expression of outer membrane protein PagP, wild type

   Depositor

   Karen Fleming

   Article

   Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

   Insert

   PagP (pagP E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N-terminal signal sequence not included and repla…

   Promoter

   T7

   Available since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJS61

   Plasmid

   #207585

   Purpose

   GFP-TolA expression

   Depositor

   Colin Kleanthous

   Article

   Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

   Insert

   GFP-TolA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHT-trxA.ads

   Plasmid

   #47383

   Purpose

   Expresses ads with trxA tag under Pgrac promoter (inducible by IPTG) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Insert

   ads
   

   Use

   Tags

   6xHis and trxA

   Expression

   Bacterial

   Mutation

   Promoter

   Pgrac

   Available since

   Oct. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJM105: pTac RFP on pCM66T

   Plasmid

   #74746

   Purpose

   pCM66T with promoter pTac driving RFP expression (maxRFP = BBa_E1010)

   Depositor

   Mary Lidstrom

   Insert

   RFP (BioBrick part number BBa_E1010)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTac

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLsCas13a-gRNA-NT

   Plasmid

   #164857

   Purpose

   Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   LsCas13a-gRNA-NT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. OPEN-HLA-B*07:02-BirA

   Plasmid

   #239752

   Purpose

   E. coli expression of BirA tagged HLA-B*07:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-B*07:02 (HLA-B Human)
   

   Use

   Tags

   BirA substrate peptide

   Expression

   Bacterial

   Mutation

   G120C

   Promoter

   T7

   Available since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBAD-GreenPy1Highest

   Plasmid

   #239049

   Purpose

   Bacterial expression of highest affinity green fluorescent pyruvate biosensor GreenPy1Highest

   Depositor

   Robert Campbell

   Article

   Imai et al bioRxiv 2025.04.17.649293

   Insert

   GreenPy1Highest
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBAD-GreenPy1High

    Plasmid

    #239050

    Purpose

    Bacterial expression of high affinity green fluorescent pyruvate biosensor GreenPy1High

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1High
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBAD-GreenPy1Lowest

    Plasmid

    #239052

    Purpose

    Bacterial expression of lowest affinity green fluorescent pyruvate biosensor GreenPy1Lowest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Lowest
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. OPEN-A*24:02

    Plasmid

    #235133

    Purpose

    E. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN-HLA-A*24:02 (HLA-A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G120C

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. OPEN-HLA-A*01:01

    Plasmid

    #235134

    Purpose

    E. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN-HLA-A*01:01 (HLA-A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G120C

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. OPEN-HLA-A*11:01-BirA

    Plasmid

    #235135

    Purpose

    E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN-HLA-A*11:01 (HLA-A Human)
    

    Use

    Tags

    BirA

    Expression

    Bacterial

    Mutation

    G120C

    Promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJS119

    Plasmid

    #207592

    Purpose

    GFP-TonB deltaII expression

    Depositor

    Colin Kleanthous

    Article

    Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    GFP-TonB deltaII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJS104

    Plasmid

    #207589

    Purpose

    GFP-TolA deltaII expression

    Depositor

    Colin Kleanthous

    Article

    Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    GFP-TolA deltaII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJS70

    Plasmid

    #207586

    Purpose

    GFP-TolA H22A expression

    Depositor

    Colin Kleanthous

    Article

    Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    GFP-TolA H22A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H22A

    Promoter

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCA528-PPxY1-GS-WW3e

    Plasmid

    #166705

    Purpose

    expresses an N-terminal fusion of AMOT PPxY1 to NEDD4L WW3 in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    AMOT PPxY1 fused to NEDD4L WW3
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUG-302

    Plasmid

    #104731

    Purpose

    Level 2 construct

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    HIS-Ubq_UBA1_HA-UBC11_EL3
    

    Use

    Tags

    HIS-Ubq, HA-E2

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-NlaCascade-NLS-6xHis

    Plasmid

    #180213

    Purpose

    Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    NlaCas5-Cas8-Cas7
    

    Use

    Tags

    6XHis and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge