31,059 results
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Plasmid#8324DepositorAvailable SinceJune 17, 2005AvailabilityAcademic Institutions and Nonprofits only
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pVRb03_up1198
Plasmid#49706DepositorInsertsfgfp
UseSynthetic BiologyExpressionBacterialPromoterPecf03_up1198Available SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCYP15
Plasmid#212848PurposeExpresses the Micromonospora cremea LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP03
Plasmid#212836PurposeExpresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHS1068
Plasmid#205962PurposeNZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promotersDepositorInsertDinG HNH proteins and CRISPR array
ExpressionBacterialPromoterLac (proteins), pJ23119 (crRNA)Available SinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
aCE120
Plasmid#204491PurposeFigure 1. reporter circuitDepositorInsertsEBFP2
mKate2
EYFP
UseSynthetic BiologyTagsnoneExpressionBacterial and MammalianPromoterhEF1aAvailable SinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-C_mScarlet-I
Plasmid#204110PurposeType 0 CDS partDepositorInsertmScarlet-I red fluorescent protein
ExpressionBacterialMutationNoneAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLRD26
Plasmid#208273PurposeProduction of levopimaradiene in E. coli MEV20DepositorInsertstHMGR
LPS
ExpressionBacterialAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLRD12
Plasmid#208264PurposeProduction of miltiradiene in E. coli MEV20DepositorInsertstHMGR
cldB
smKAL
ExpressionBacterialAvailable SinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
SmtA Synechococcus elongatus PCC 7942 Metallothionein
Plasmid#200298PurposeBacterial expression plasmids for production of recombinant Synechococcus elongatus PCC 7942 MetallothioneinDepositorInsertGene of metallothionein with Chiting Binding Domanin-Intein (SYNPCC7942_RS13750 Synechococcus elongatus)
TagsChiting Binding Domanin-InteinExpressionBacterialAvailable SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDSP23
Plasmid#199372PurposeEncodes dual cistronic nuclear localized GCaMP6s and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::GCaMP6s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
ExpressionBacterialMutationno mutations, except added subcellular localizati…Available SinceApril 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJMH147
Plasmid#186647PurposeaadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1 between homology arms for pilADepositorArticleInsertaadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKP579
Plasmid#159598PurposeExpress Cba and Cbi proteinDepositorInsertcba and cbi
ExpressionBacterialMutationnoneAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH33
Plasmid#175233PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4-ICL1
Plasmid#105822PurposeExpression of Mycobacterium tuberculosis icl1 recombinant proteinDepositorInserticl1
TagsHis tag with TEV cleavageExpressionBacterialPromoterT7Available SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-dSpCas9-HF1-VP64-6xHis
Plasmid#92118PurposeExpression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, N497A, R661A, Q695A, H840A, Q926APromoterT7Available SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Thioredoxin TEV cloning vector with BioBrick polycistronic restriction sites (9T)
Plasmid#48292DepositorTypeEmpty backboneTagsHis6 and ThioredoxinExpressionBacterialAvailable SinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7-PLK1
Plasmid#46312DepositorInsertsp19
PLK1 DNA-1
linker
PLK1 DNA-2
UsePro-sirnaTags6XHis and GSTExpressionBacterialAvailable SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC201
Plasmid#129387PurposeA variant of the pSCrhaB2 vector with the oriR6K and mob genes from pGPΩ-TP. Used for homologous recombination of the rhamnose-inducible system into the chromosome of Burkholderia cenocepacia K56-2.DepositorInsertoriR6K and oriT
ExpressionBacterialMutationN/APromoterN/AAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-3xAU1
Plasmid#129572PurposeEmpty backbone for AU1 tagDepositorTypeEmpty backboneTags3xAU1 and GSTExpressionBacterialAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-SF3B1-NTerm
Plasmid#97425PurposeExpresses human SF3B1 (AA 1-500) with N-term GST tag for inducible expression in E.coliDepositorInsertSF3B1-N-term (AA1-500)
TagsGSTExpressionBacterialMutationSilent mutation at nt876, aa289 (AGG to AGA)PromotertacAvailable SinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFTK043
Plasmid#171315PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInserteGFP fluorescent reporter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA-GFP-R5
Plasmid#106256PurposeConstitutively expresses GFP fused with silaffin-R5 silica affinity peptideDepositorInsertGreen fluorescent protein
TagsAdded silaffin R5 peptide to C terminus of GFP af…ExpressionBacterialAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-StefinA
Plasmid#182403PurposeBacterial expression of Vesicle Nucleating peptide-stefinA fusionDepositorInsertVNp-stefin (CSTA Human)
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS415-KEX2
Plasmid#10996DepositorAvailable SinceDec. 29, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-Q
Plasmid#64980PurposeExpresses the E108Q mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E108QAvailable SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-RiboJ-LacZa-T7
Plasmid#173224PurposepFGC-T7-RJBB to clone genes by Golden Gate (BsaI). To be used in cell-free systemsDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceMarch 9, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
SPIN4
Plasmid#73239PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGST TCF4 DBD
Plasmid#16488DepositorAvailable SinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
p3992 pET ubcH5
Plasmid#8652DepositorInsertUbcH5 (UBE2D1 Human)
ExpressionBacterialAvailable SinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pL90-Nat-2xHO
Plasmid#139069PurposeCRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selectionDepositorInsertTwo guide RNAs targeting the HO endonuclease
ExpressionBacterial and YeastPromoterTDH3Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT106b3
Plasmid#31393DepositorInsertred light inverter with lacZ output
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
TLS 4: hTLS-CHOP.pBSKS(+)
Plasmid#21830DepositorInsertTLS-CHOP (FUS Human)
ExpressionBacterialAvailable SinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-arrO-N
Plasmid#128143PurposeDinoflagellate backbone with rifampicin resistant gene optimized for Oxyrrhis marinaDepositorInsertRifampin ADP-ribosylating transferase + potential promoter
ExpressionBacterialAvailable SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pChlr-pNEB193
Plasmid#52136PurposeA backbone plasmid for creation of additional PUF repeat modulesDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
CDK2_HUMAN_D0
Plasmid#79726PurposeThis plasmid encodes the kinase domain of CDK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTHS
Plasmid#166938PurposeToehold switch multi-level gene expression controller (MLC)DepositorInsertGenetic circuit expressing GFP- Toehold switch
UseSynthetic BiologyExpressionBacterialPromoterPTACAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUdO4t
Plasmid#210249Purposelow-copy plasmid for bacterial expressionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pV2A-T
Plasmid#153022PurposeSingle-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoterDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Endophilin SH3 domains
Plasmid#47401DepositorInsertEndophilin
TagsGSTExpressionBacterialMutationSH3 domain (aa 292-352)PromotertacAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only