32,826 results
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Plasmid#123158PurposeArsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR-ABS67, PhrpLE, PrinAAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
As2
Plasmid#123160PurposeArsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR-ABS84, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109As-gfp
Plasmid#123143PurposeAn arsenic sensor with optimized intracellular receptor density. pSB3K3 carrying J109-30arsR-t-ParsR-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsRAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluSnFR
Plasmid#119828PurposeProduction of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluSnFR
TagsGSTExpressionBacterialPromoterT7Available SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGC4593
Plasmid#110206PurposeAccessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)DepositorInsertsTEV protease
aaRS
ExpressionBacterialPromoterPbad and PtetAvailable SinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pClodANL-Cherry
Plasmid#107870PurposeBacterial expression of cherry-NanoLucDepositorInsertCherry (red fluorescent protein)
UseLuciferaseTagsNanoLucExpressionBacterialPromoterpro DAvailable SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT3003
Plasmid#122537PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (pisy-1) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTn7·X-nia
Plasmid#122592PurposePlasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion siteDepositorInsertxylS/Pm->Nia
UseSynthetic Biology; Tn7 genomic integrationExpressionBacterialPromoterPmAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-R399E
Plasmid#122691PurposeExpress His-tagged PKM2 (R399E) protein in bacteria.DepositorAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-G415R
Plasmid#122693PurposeExpress His-tagged PKM2 (G415R) protein in bacteria.DepositorAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-APIC-Cas9
Plasmid#121657Purposegateway cloning destination vector for expressing enhancer driven Cas9DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del4 pET28C
Plasmid#122118PurposeExpression Brg1 amino acids 1446-1647 in bacteriaDepositorAvailable SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBLO 9.2_bCas9-CP199
Plasmid#121496PurposeTetR_tetR/tetA promoters_dCas9-CP_CM_P15ADepositorTypeEmpty backboneExpressionBacterialPromoterp15AAvailable SinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRG/III-hs-MBP-T43NTR-TrxA-H10
Plasmid#121677PurposeBacterial expression of neurotensin receptor fusion proteinDepositorInsertneurotensin receptor (Ntsr1 Rat)
TagsTrxA-H10ExpressionBacterialMutationdeleted amino acids 1-42PromoterlacAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-tev-APEX2
Plasmid#117751PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Cas12c2-noncoding
Plasmid#120877PurposeExpresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.DepositorInsertNoncoding sequences for CRISPR-Cas12c2
UseCRISPRExpressionBacterialPromoterlacAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEJS1026-pMCSG7-HpaCas9
Plasmid#121540PurposeExpresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cellsDepositorInsertHpaCas9
ExpressionBacterialAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEJS1027-pMCSG7-SmuCas9
Plasmid#121541PurposeExpresses a 6X-His tagged type II-C Cas9 from S. muelleri in bacterial cellsDepositorInsertSmuCas9
ExpressionBacterialAvailable SinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMVA2
Plasmid#119951PurposeExpresses recombinant MVA pathway in Escherichia coliDepositorInsertsmvaE
mvaS
mvaK1
mvaK2
mvaD
idi
ExpressionBacterialMutationA110GAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMEG_YLCas9_empty
Plasmid#117826PurposeCRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolyticaDepositorInsertYlpTEF_hCas9_YlCyc1TT
ExpressionBacterial and YeastAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1069
Plasmid#119244Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1290
Plasmid#119267Purposeconstruction intermediateDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-HA
Plasmid#119751PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal HA tag.DepositorTypeEmpty backboneTagsGST and HAExpressionBacterialPromotertac promoterAvailable SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNP1 tdTomato toehold switch
Plasmid#107354PurposeExpresses tdTomato (a DsRed derivative) in Ecoli off a T7 promoter when pNP1 tdTomato trigger is co-expressedDepositorInserttoehold switch 5 + tdTomato
ExpressionBacterialPromoterT7Available SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_51
Plasmid#109249Purposep15A BbsI cloning vectorDepositorInsertsfGFP
ExpressionBacterialPromoterPJ23105Available SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
B5-2
Plasmid#111481PurposeArg-tRNA synthetaseDepositorInsertArg-tRNA synthetase
Tags6xHisExpressionBacterialAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
6CDG (GID4)
Plasmid#119961PurposeBacterial expression for structure determinationDepositorInsertGID4 (C17orf39 Human)
Tags6xHis-TEVExpressionBacterialMutationcontains amino acids 124-289PromoterT7Available SinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
6CC8 (MBD3)
Plasmid#119959PurposeBacterial expression for structure determinationDepositorInsertMBD3 (MBD3 Human)
TagsGSTtag, 6xHis and ThrombinExpressionBacterialMutationMBD domain (contains residues 1-71)PromoterT7Available SinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIBA2-SpyTag-MBP
Plasmid#107422PurposeControl construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.DepositorInsertMaltose-binding protein (MalE)
TagsOmpA signal peptide, SpyTag, and StrepIIExpressionBacterialPromoterTetAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1335
Plasmid#119269Purposerfp "test" strainDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only