31,059 results
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Plasmid#139467PurposeT7 RNAP-driven expression of WT trigger RNA with mutation at 42DepositorInserttarget_42_mut_G
ExpressionBacterialAvailable SinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Cas12c2-noncoding
Plasmid#120877PurposeExpresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.DepositorInsertNoncoding sequences for CRISPR-Cas12c2
UseCRISPRExpressionBacterialPromoterlacAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
prCAG-RBS"m1"-sfGFP
Plasmid#191400PurposeExpression of 47 rCAG repeats fused to a translational unit over-expressing superfolder GFP using a strong RBS. The RBS contains mutation (m1)DepositorInsert47xCAG RBS"m1"-sfGFP
UseSynthetic BiologyExpressionBacterialPromoterPLtet0-1Available SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEV-Gly-RadA C46
Plasmid#128736PurposeExpresses PhoRadA intein split at residue 46 from the C-terminalDepositorInsertRadA
TagsHisx6-MBPExpressionBacterialPromoterT7Available SinceAug. 12, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMTP130 (pTA106_TnsABC(Tn6900))
Plasmid#164261PurposeVector expressing the TnsA, TnsB, and TnsC transposition proteins from the Tn6900 derivative element with a lactose promoter.DepositorInserttnsA, tnsB, and tnsC from Tn6900 derivative from A. salmonicida S44 pS44-1
ExpressionBacterialMutation(tnsA) Changed Alanine 125 to Aspartic acidPromoterLactoseAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2_ZIKV NS4B
Plasmid#145367PurposeCell-free/mammalian expression vector for Zika virus NS4Bprotein with N-term mCherryDepositorInsertZIKV NS4B
Tags8xHis and mCherryExpressionBacterialPromoterT7- CMV/CAGAvailable SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS21: pHelper(PmcCAST)_entry
Plasmid#168154PurposeInducible expression of PmcCAST proteins. Two BsaI sites for spacer cloning.DepositorInsertsPmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-AP
Plasmid#61830PurposeEncodes SNAP-AP fusion for tagging endogenous target for SNAP-SiMPullDepositorInsertSNAP-AP
UsePcr templateExpressionBacterial and YeastAvailable SinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQnl_Pcphy23
Plasmid#191354PurposeClostridium plasmid expressing NanoLuc reporter from moderate, constitutive promoterDepositorInsertPcphy23-NanoLuc
ExpressionBacterialPromoterPcphy23Available SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-GB1 Leu5Met
Plasmid#128686PurposeExpresses GB1 Leu5Met mutantDepositorInsertspg
TagsHisExpressionBacterialPromoterT7Available SinceApril 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJUMP18-cueR_O
Plasmid#127026PurposeBasic Part O- ORF; cueR; transcription factorDepositorInsertPart cueR_o
UseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Sos1 (PRM) - Cys (XSB364)
Plasmid#78506PurposeConstruct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labelingDepositorInsertSos1 -Cys (PRM) (SOS1 Human)
TagsGST followed by a prescission protease claevage s…ExpressionBacterialAvailable SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mEGFP-YAP-N-IDR
Plasmid#166440PurposeBacterial expression of 6x His tagged mEGFP-YAP1-N-IDR fusion proteinDepositorInsertmEGFP, YAP-N-IDR(1-164) (YAP1 Human)
ExpressionBacterialAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-103 FL
Plasmid#47090DepositorAvailable SinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pKM263-EcPPXc
Plasmid#38328DepositorInsertE. coli polyphosphatase (ppx Escherichia coli)
Tags6xHIS, GST, and TEVExpressionBacterialMutationC-terminus of E. coli polyphosphatase (described …PromoterT7Available SinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCVD433
Plasmid#63756PurposeDerivative of pACYC184 M1uI linkers inserted into EcoRV site inactivating Tc resistance gene. Now TcsDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
GAPDHS
Plasmid#39010PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTn7xKS-mPlum
Plasmid#117396PurposeTn7 tagging vector pTn7xKS with mPlum expression scaffoldDepositorInsertmPlum
UseSynthetic BiologyExpressionBacterialPromoterPtacAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbB8a-GFP
Plasmid#35361DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialPromoterBADAvailable SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pColdI-GST-mGTSF1l
Plasmid#214817PurposeBacterial expression of GST-tagged mouse GTSF1lDepositorAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCLC-mEos2
Plasmid#38010DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
TagsmEos2ExpressionBacterial and MammalianPromoterCMVAvailable SinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3-AD009
Plasmid#116850PurposeExpresses araC constitutively and alpha-hemolysin under a pBAD promoter; alternate name pSB1A2-AD009DepositorInsertsAraC
alpha-HL
UseSynthetic BiologyExpressionBacterialPromoterpBADAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
RBM15B_3xRRM_pGEX
Plasmid#135125PurposeRecombinant expression of RBM15B with dual-affinity tagDepositorAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
11 pCOLI_A_MBP
Plasmid#160247PurposeN-term MBP 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneTags6xHis and MBPExpressionBacterialPromoterT7Available SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-160-GCN4
Plasmid#74608PurposeExpresses artificially dimerized Hook domain of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-160 (HOOK3 Human)
Tags6x His, GCN4, Strep II, and superfolder GFPExpressionBacterialAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
Pr-deGFP
Plasmid#67743PurposePr promoter expressing deGFPDepositorInsertUTR1-deGFP-T500
UseSynthetic BiologyExpressionBacterialPromoterOR2-OR1-PrAvailable SinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUWL201PW-creDE
Plasmid#112241PurposeStreptomyces/E.coli shuttle expression vector encoding creD and creEDepositorInsertscreD
creE
ExpressionBacterialAvailable SinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKCyGe2
Plasmid#167515PurposeBroad-Host range expression vector, RK2 and pUC origin, CymRAM repressor, PCymRC promoter controlling GmR, Kanamycin Resistance Marker.DepositorInsertCymRAM-GmR
UseSynthetic BiologyExpressionBacterialPromoterPCymRCAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pBAD-OleD
Plasmid#59372PurposeInducible expression of OleD (DQ195536) under arabinose promoterDepositorInsertOleD-His
ExpressionBacterialPromoterpBADAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJMP1071
Plasmid#119245Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1 mNFATc2
Plasmid#17858DepositorInsertNFATc2 (Nfatc2 Mouse)
ExpressionBacterialAvailable SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mCerulean LIC cloning vector (MBP-mCerulean)
Plasmid#29751DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), MBP, TEV cleavage site, a…ExpressionBacterialAvailable SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAT3_G-alpha
Plasmid#109948PurposeProtein expression and purification of GNAT3_G-alphaDepositorInsertGNAT3_G-alpha (GNAT3 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
C2-82 pComb3xss anti-cFos nanobody
Plasmid#145838PurposeBacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-cFos (Human) recombinant llama nanobody C2-82 (FOS L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAH01SpR
Plasmid#128159PurposepCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDSDepositorTypeEmpty backboneExpressionBacterialPromoterPtetAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_105_Venus_195
Plasmid#39821DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hdNadV
Plasmid#83362PurposeExpresses nadV from Haemophilus ducreyi in Escherichia coliDepositorInsertnadV
TagsnoneExpressionBacterialPromoterT7Available SinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKB239
Plasmid#170034PurposeEncodes LacI-controlled expression of surface-displayed GNLY and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only