30,808 results

1. pETM11-ACS

   Plasmid

   #108943

   Purpose

   heterologous expression of ACS of E. coli in E. coli

   Depositor

   Frank Schulz

   Article

   Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

   Insert

   Acetyl-CoA synthetase (ACS)
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCellFree_G06_ZIKV E

   Plasmid

   #145351

   Purpose

   Cell-free expression vector for Zika virus Envelope protein with C-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV Envelope
   

   Tags

   mCherry and 8xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFGC5941-PacI

   Plasmid

   #44182

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACYC-GST

   Plasmid

   #62329

   Purpose

   E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Tags

   Glutathione S-transferase (GST) tag and TEV prote…

   Expression

   Bacterial

   Promoter

   T7-lac0

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET29_LuxSit_i

   Plasmid

   #194299

   Purpose

   Bacterial expression of the de novo luciferase, LuxSit-i

   Depositor

   David Baker, Andy Yeh

   Article

   Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

   Insert

   LuxSit-i
   

   Expression

   Bacterial

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAMK2A

   Plasmid

   #38971

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CAMK2A (CAMK2A Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

7. MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5

   Plasmid

   #135454

   Purpose

   Bacterial expression of TEVΔ protease

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
   

   Tags

   MBP, His6

   Expression

   Bacterial

   Mutation

   S219V mutation improves stability. Truncated form…

   Promoter

   tac promoter

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLS.419

   Plasmid

   #187208

   Purpose

   Expresses wild-type retron Eco4 ncRNA, and Cas1 + Cas2

   Depositor

   Seth Shipman

   Article

   Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

   Inserts

   Retron Eco4 ncRNA
   Cas1 + Cas2
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7/Lac

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pH6HTC_PTBP1-Halo

   Plasmid

   #175302

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   PTBP1 (PTBP1 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. AfNitB_pET29b

    Plasmid

    #215401

    Purpose

    Expression construct for AfNitB gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    AfNitB
    

    Expression

    Bacterial

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPPC005

    Plasmid

    #171140

    Purpose

    For integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    XylS-Pm
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Pm

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-EPN51 (R40A)

    Plasmid

    #80109

    Purpose

    Late1-O3-33-myc-PH(R40A)

    Depositor

    Neil King

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-51 (R40A)
    

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

13. R701-X54-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D

    Plasmid

    #159562

    Purpose

    E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D (KRAS Human)
    

    Tags

    His6-MBP-tev-Avi

    Expression

    Bacterial

    Mutation

    G12D

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. HisMS2_VLP_RSV

    Plasmid

    #175958

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]

    Depositor

    Paul Freemont

    Article

    Unruh et al medRxiv 2024.04.17.24305965

    Inserts

    P gene
    M gene
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCD185

    Plasmid

    #113317

    Purpose

    dCas9, MCP-SoxS_R93A

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    dCas9 and MCP-SoxS_R93A
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    R93A

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLZ12Km2-P23R:TA:ffluc

    Plasmid

    #88900

    Purpose

    Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

    Depositor

    Thomas Proft

    Article

    Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

    Insert

    toxin-antitoxin + firefly luciferase
    

    Use

    Luciferase

    Expression

    Bacterial

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNM262

    Plasmid

    #128412

    Purpose

    Vanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    Lysostaphin (epr Staphylococcus simulans)
    

    Use

    Synthetic Biology

    Tags

    amyE SP

    Expression

    Bacterial

    Promoter

    Pspank(V)

    Available Since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. miniTn7(Gm)PA1/04/03-ecfp-a

    Plasmid

    #111619

    Purpose

    eCFP Fluorescent tagging of bacteria at their Tn7-site

    Depositor

    Tim Tolker-Nielsen

    Article

    Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

    Insert

    PA1/04/03-ecfp
    

    Expression

    Bacterial

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. LbCas12a-gRNA_BbsI_CAM

    Plasmid

    #183222

    Purpose

    LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    LbCas12a guide RNA containing BbsI sites in spacer sequence
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-KG-Itch/2793

    Plasmid

    #11434

    Depositor

    Allan Weissman

    Article

    Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

    Insert

    Itch (Itch Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    n/a

    Available Since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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