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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#68

Plasmid

#153527

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#68

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#68

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK569

Plasmid

#110614

Purpose

BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

E2-Crimson

Use

Tags

Expression

Bacterial

Mutation

V32M (see comments below)

Promoter

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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MS2-Snapf

Plasmid

#65860

Purpose

Expresses RNA-binding protein MS2 with fast SNAP fusion tag

Depositor

Aaron Hoskins

Article

Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

Inserts

MS2
Fast SNAP

Use

Tags

HA and NLS

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only
pMRE-Tn5-136

Plasmid

#118522

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn5 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-HiGlpG

Plasmid

#158538

Purpose

Bacterial expression of rhomboid protease HiGlpG

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

HiGlpG

Use

Tags

GST tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-AGO3

Plasmid

#24318

Depositor

Carl Novina

Article

Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

Insert

AGO3 (AGO3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pNIC-CH10-GG

Plasmid

#215807

Purpose

Cloning of target gene with C terminal non cleavable His10 tag

Depositor

Frank von Delft, ASAP Discovery

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiex-1 Myosin VI HMM_SNAP

Plasmid

#82716

Purpose

Expresses Myosin VI HMM with a C-terminal SNAP tag.

Depositor

Sivaraj Sivaramakrishnan

Article

Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

Insert

Myosin VI

Use

Tags

6xHis Tag, FLAG purification tag, and SNAP tag

Expression

Bacterial and Insect

Mutation

Promoter

T7 Promoter

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pKB226

Plasmid

#170016

Purpose

Encodes LacI-controlled expression of surface-displayed defensin HNP-1 and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
Defensin HNP-1

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196)-Strep

Plasmid

#196208

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Use

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, D40G)-Strep

Plasmid

#196210

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Use

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

D40G

Promoter

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pVE2_11_p40S_QfDBD_VP16AD_cp_phl_DsRed_0xQUAS

Plasmid

#154229

Purpose

Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_phl (no QUAS control)

Depositor

Arnold Driessen

Article

Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

Insert

p40S:QfDBD-VP16AD-GFP-NLS

Use

Synthetic Biology; Fungal expression

Tags

GFP-NLS; DsRed-SKL

Expression

Bacterial

Mutation

Promoter

p40S (AN0465 A. nidulans)

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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S5_EcoRV_Ef1a_AscI

Plasmid

#128469

Purpose

Ef1a promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

Ef1a promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21 E2F1

Plasmid

#90313

Purpose

Expresses human E2F1 in bacteria

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 (E2F1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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IBMc090

Plasmid

#161937

Purpose

Carries engineered transposon for IBM

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-knt-P-R

Plasmid

#189849

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P-loxP-TT-loxP-R-knt

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

P (T7 phage gene 10)

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-Kaede (Kaede-A69T)

Plasmid

#99228

Purpose

Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-Kaede

Use

Tags

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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RAB43

Plasmid

#25513

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

RAB43 (RAB43 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 30, 2010

Availability

Industry, Academic Institutions, and Nonprofits
MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A

Plasmid

#110555

Purpose

pFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.

Depositor

Martin Pule

Article

Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

Insert

Anti-RD114 heavy chain

Use

Tags

mIgG2A

Expression

Bacterial and Mammalian

Mutation

Promoter

EF-1 alpha core promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET28CLY5

Plasmid

#21764

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 5 SGGSGG repeats

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only