30,307 results

1. pET-AqpZ-ALFA

   Plasmid

   #212310

   Purpose

   Expression of AqpZ with ALFA tag

   Depositor

   Arthur Laganowsky

   Article

   Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

   Insert

   Aquaporin Z
   

   Use

   Tags

   ALFA tag and Strep-Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUK

   Plasmid

   #64185

   Purpose

   harbours marker genes URA3 and LYS2

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Aug. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p11d-tRPA-32Ala9

   Plasmid

   #102616

   Purpose

   Expression of human RPA with an alanine substituted phosphorylation domain (with the following mutations in RPA32 :S8,11-13,23,29,33,39A,T21A).

   Depositor

   Marc Wold

   Inserts

   RPA1 (RPA1 Human)
   RPA3 (RPA3 )
   RPA2 (RPA2 )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   with an alanine substituted phosphorylation domain

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TJF229

   Plasmid

   #132575

   Purpose

   pZE1-oxySp-mCherry-proD-oxyR

   Depositor

   Timothy Lu

   Article

   Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

   Inserts

   oxySp-mCherry-TermT1
   proD-oxyR-TermT1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   oxySp and proD

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEM cAgg (CT#689)

   Plasmid

   #13945

   Depositor

   Cliff Tabin

   Article

   Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

   Insert

   Aggrecan (ACAN Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Nucleotides 113-800

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

6. GFP-(SIM)3

   Plasmid

   #126955

   Purpose

   Bacterial expression plasmid for eGFP-(SIM)3 client to assess SUMO-SIM scaffolds.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   EGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
   

   Use

   Tags

   eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   unknown

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMG046 pETARA-CK1α-His

   Plasmid

   #154074

   Purpose

   Expresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   CSNK1A1 (CSNK1A1 Human)
   

   Use

   Tags

   GST and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SIRT6

   Plasmid

   #28271

   Depositor

   Cheryl Arrowsmith

   Insert

   SIRT6 (SIRT6 Human)
   

   Use

   Tags

   His tag with thrombin cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pET51b(+)-SNAP-CLIP

   Plasmid

   #127180

   Purpose

   Bacterial expression of the fused self-labeling tags SNAP-tag and CLIP-tag

   Depositor

   Kai Johnsson

   Article

   Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

   Insert

   SNAP-CLIP
   

   Use

   Tags

   HisTag and StrepTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKL002

    Plasmid

    #98921

    Purpose

    pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Inserts

    PROPS
    GCaMP6f
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUNIV-EGFP

    Plasmid

    #24706

    Depositor

    Cynthia Czajkowski

    Article

    Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

    Insert

    EGFP (eGFP Aequoria victoria)
    

    Use

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Available since

    Dec. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBAD-hfYFP

    Plasmid

    #186522

    Purpose

    Hyperfolder YFP fluorescent protein: bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Hyperfolder YFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

    Promoter

    araB

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX-4T2-Rheb

    Plasmid

    #15889

    Depositor

    John Blenis

    Article

    Tee et al Curr Biol. 2003 Aug 5. 13(15):1259-68.

    Insert

    Rheb (RHEB Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pGEX6P1-UBE2S-UBD

    Plasmid

    #66713

    Purpose

    Expresses the UBE2S-UBD fusion protein in E. coli

    Depositor

    David Komander

    Article

    Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

    Inserts

    e2-epf (UBE2S )
    isot (USP5 )
    

    Use

    Tags

    GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

15. PNMT

    Plasmid

    #64078

    Purpose

    Expression of human Phenylethanolamine N-methyltransferase

    Depositor

    Vern Schramm

    Article

    Stratton et al ACS Chem Biol. 2017 Feb 17;12(2):342-346

    Insert

    Phenylethanolamine N-methyltransferase (PNMT Synthetic, Human)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pRF-McbR

    Plasmid

    #49365

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    McbR repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPBC-G6f

    Plasmid

    #62811

    Purpose

    pPBC-G6f expresses PiggyBac inverted terminal repeat-flanked GCaMP6f under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-GCaMP6f-ITR
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX 6P1 Cyclin A (555)

    Plasmid

    #61844

    Purpose

    bacterial expression of GST cyclin A

    Depositor

    Jonathon Pines

    Insert

    cyclin A (CCNA2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    L2V

    Promoter

    tac

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pJMH143

    Plasmid

    #186646

    Purpose

    aadA.co-TaadA-PJ23117-eYFP.co between homology arms for pilA

    Depositor

    Adam Arkin

    Article

    Jacob M. et al bioRxiv

    Insert

    aadA.co-TaadA-PJ23117-eYFP.co
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-gSTEP1

    Plasmid

    #159376

    Purpose

    Expresses gSTEP1 in E. coli

    Depositor

    Roberto Chica

    Article

    Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

    Insert

    gSTEP1
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge