Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,167 results

Showing: 9961 - 9990 of 32167 results

  6. pJK210

    Plasmid
    #73325
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioD
    Depositor
    T. J. Kappock
    Insert
    violacein biosynthesis protein VioD
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73325-plasmid-map-sequence-id-135033

    Enlarge

  7. roGFP_iE_C147S_pQE30

    Plasmid
    #48631
    Purpose
    Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 147 to Serine mutation (roGFP C147S, E147b, C204)
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    roGFP1
    Tags
    HisTag
    Expression
    Bacterial
    Mutation
    insertion E147B, C147S
    Available Since
    Oct. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    48631-plasmid-map-sequence-id-70379

    Enlarge

  13. Z-VCP-10

    Plasmid
    #166559
    Purpose
    scFv of a human scaffold targeting Transitional endoplasmic reticulum ATPase. Antigen coverage aa 1-806 of 806, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166559-plasmid-map-sequence-id-329267

    Enlarge

  16. pDR111_Bgal NLS

    Plasmid
    #188396
    Purpose
    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Insert
    lacZ
    Tags
    PhoD secretion peptide and SV40 NLS
    Expression
    Bacterial
    Promoter
    Phyper spank
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    188396-plasmid-map-sequence-id-373478

    Enlarge

  18. TPH2

    Plasmid
    #73243
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TPH2 (TPH2 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73243-plasmid-map-sequence-id-137195

    Enlarge

  19. pBiex PKC - Peptide 8 GFP RFP

    Plasmid
    #83564
    Purpose
    Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available Since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    83564-plasmid-map-sequence-id-166406

    Enlarge

  24. RP1B-PP1gamma

    Plasmid
    #51770
    Purpose
    Expresses PP1 gamma residues 7-323
    Depositor
    Wolfgang Peti
    Insert
    pp1 gamma
    Tags
    His, TEV protease site, and Thio-6
    Expression
    Bacterial
    Mutation
    Contains PP1 gamma residues 7-323
    Promoter
    T7-lac
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. pHH0103 NEDD4L WW domain #2

    Plasmid
    #104205
    Purpose
    Bacterial expression of WW domain #2 from NEDD4L
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    NEDD4L WW domain #2 (NEDD4L Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104205-plasmid-map-sequence-id-203673

    Enlarge

Showing: 9961 - 9990 of 32167 results