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pET-32a(+)-NRG1B1

Plasmid

#120287

Purpose

Produce human recombinant NRG1 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

NRG1-beta 1

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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1457_pDEST_miniTol2_R4-R2_Cryst_mCherry

Plasmid

#171796

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLys-M1

Plasmid

#109382

Purpose

Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-VioB-mCherry

Expression

Bacterial

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHS9_10xHis_MBP_TEV_dCas9

Plasmid

#244831

Purpose

Bacterial expression of catalytically inactive Cas9 (dCas9) for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dCas9 (WT)

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D10A/H840A

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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EML6428_ pRL662-eGFP

Plasmid

#234350

Purpose

Expressed eGFP(S65T) in agrobacteria for tracing in planta

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

SpeR

Expression

Bacterial

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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YopH-pNIC-Bsa4

Plasmid

#92063

Purpose

pNIC expression vector encoding gene for YopH

Depositor

Josh Beckham

Insert

YopH

Tags

6x His Tag

Expression

Bacterial

Mutation

Codon optimized E. coli

Available Since

Jan. 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pReporter_5

Plasmid

#60566

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_5

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pSC-SmBiT

Plasmid

#207632

Purpose

A plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiT

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

photocaged SpyCatcher-SmBiT

Tags

6xHis

Expression

Bacterial

Mutation

Amber stop codon at SC's critical lysine

Promoter

T7

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pReporter_7

Plasmid

#60567

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_7

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA

Plasmid

#64983

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pRSETA-alphaFlincG

Plasmid

#19735

Depositor

Wolfgang Dostmann

Article

Nausch et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

alpha-FlincG

Use

Protein expression

Tags

His

Expression

Bacterial

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pRSETA-betaFlincG

Plasmid

#19736

Depositor

Wolfgang Dostmann

Article

Nausch et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

beta-FlincG

Tags

His

Expression

Bacterial

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pBS-tRNA-Ser

Plasmid

#174318

Purpose

For in vitro transcription of mouse tRNAs

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

tRNA-ser

Expression

Bacterial

Promoter

T7

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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MB 45t3 thsS(t3)R-sfGFP_mCherry

Plasmid

#232470

Purpose

Optimized thiosulfate sensor with fluorescent reporter (GFP), constitutive mCherry

Depositor

Mikhail Shapiro

Article

Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:

Inserts

thsS(t3)
thsR
PphsA342
sfGFP
AxeTxe
mCherry

Expression

Bacterial

Mutation

Contains the following mutations K286Q, Q350H, I…

Promoter

J23100 (TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC), J23…

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 isolate:Wuhan-Hu-1 nsp3 macrodomain

Plasmid

#228643

Purpose

Bacterial expression of the SARS-CoV-2 nsp3 macrodomain for crystallization of SARS-CoV-2 nsp3 macrodomain in P 4 3

Depositor

ASAP Discovery, Frank von Delft

Insert

SARS-CoV-2 NSP3 macrodomain (ORF1ab SARS-CoV-2)

Tags

His-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pnEA-NpM-HsNot6_AF

Plasmid

#148738

Purpose

Bacterial Expression of HsNot6

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot6 (CNOT6 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot6-D491A_AF

Plasmid

#148659

Purpose

Bacterial Expression of HsNot6-D491A

Depositor

Elisa Izaurralde

Article

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot6-D491A (CNOT6 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot7-D40AE42A_AF

Plasmid

#148675

Purpose

Bacterial Expression of HsNot7-D40AE42A

Depositor

Elisa Izaurralde

Article

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot7-D40AE42A (CNOT7 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-tRNA-Gly

Plasmid

#174317

Purpose

For in vitro transcription of mouse tRNAs

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

tRNA-gly

Expression

Bacterial

Promoter

T7

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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human p53-(1-393)

Plasmid

#24859

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Full length.

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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