30,535 results
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Plasmid#128732PurposeGateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal Cya tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only
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pSR83
Plasmid#90211PurposeExpresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
DP6
Plasmid#140446PurposeUntargetted bacterial driftDepositorInsertdnaQ926 dam seqA emrR ugi CDA1 gIII
UseTagsExpressionBacterialMutationPromoterAvailable SinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHN924
Plasmid#178885PurposeExpression of GST-His8-HRV3CDepositorInserthuman rhinovirus 3C protease
UseTagsGST, His8ExpressionBacterialMutationPromoterT7Available SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-OTUB1*
Plasmid#65441PurposeExpresses activated OTUB1 in E. coliDepositorUseTagsHis-3CExpressionBacterialMutationActive site cysteine mutated to alaninePromoterT7Available SinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRG/III-hs-MBP-CB2-HF
Plasmid#121682PurposeBacterial expression of cannabinoid receptor CB2 fusion proteinDepositorInsertperipheral cannabinoid receptor (CNR2 Human)
UseTagsHis6 Flag tagExpressionBacterialMutationPromoterlacAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLacO-ISce1
Plasmid#58505Purposeprovides 256 Lac operator repeats adjacent to the ISce1 restriction siteDepositorInsertLac repeats adjacent to 6 copies of the ISce1 restriction site
UseTagsExpressionBacterialMutationPromoterno promoterAvailable SinceOct. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVP91A-pcaHG
Plasmid#113766PurposeExpresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.DepositorInsertsβ subunit of Protocatechuate 3,4- Dioxygenase
α subunit of Protocatechuate 3,4- Dioxygenase
UseTagsMet-6xHisExpressionBacterialMutationPromoterT5 promoterAvailable SinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRT498-OtUBD
Plasmid#190089PurposeExpresses a single OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsertOtUBD
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-SCR-sgRNA-A
Plasmid#177811PurposeOverexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)DepositorInsertScrambled sgRNA targeting sequences
UseTagsExpressionBacterialMutationPromoterAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
NW9
Plasmid#133442PurposeCovalently circularized nanodisc NW9 for studying membrane proteins and viral entry.DepositorInsertNW9
UseTagsExpressionBacterialMutationPromoterAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS hTSP-5
Plasmid#13002DepositorAvailable SinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-GPD1
Plasmid#83911Purposestable overexpressionDepositorInsertglycerol-3-phosphate dehydrogenase 1 (GPD1 Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available SinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX:PGRMC1
Plasmid#136062PurposeBacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.DepositorInsertProgesterone receptor membrane component 1 (PGRMC1 Human)
UseTagsGST-PGRMC1ExpressionBacterialMutationPromoterAvailable SinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pON.mCherry
Plasmid#84821PurposeConstitutive Expression plasmid with mCherry fluorescent proteinDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterTac PromoterAvailable SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIV151
Plasmid#226180PurposeExpresses Cas9 in a backbone compatible with K. phaffii.DepositorInsertCas9
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterGAP1Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-CH (CamR, HK022)
Plasmid#45980DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTK-enhNC1.1_M3-MCS-2A-eGFP-SV40pA
Plasmid#110203PurposeRecipient vector for Cranial Neural Crest-specific expression of protein of interestDepositorInsertenhNC1.1_M3-TF_MCS-2A-eGFP-SV40pA
UseTagseGFPExpressionBacterialMutationPromoterAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huMb2Cas12a
Plasmid#174676PurposeExpresses 6xHis-MBP tagged Mb2Cas12aDepositorArticleInserthuMb2Cas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET23b-15F11-HA-mEGFP-6xHis
Plasmid#129593PurposeThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.DepositorInsertAnti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
UseTags6xHis-tagExpressionBacterialMutationPromoterT7Available SinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only