33,432 results
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Plasmid#52656PurposeExpresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.DepositorInsertTEM-1 β-Lactamase
TagsSpyCatcher and SpyTagExpressionBacterialPromoterT7 promoterAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKD‐SepRS-EFSep-5x tRNASep (B40 OTS)
Plasmid#52054PurposeUsed in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)DepositorInsertsSepRS
EF-Sep
5x tRNA Sep
ExpressionBacterialMutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…PromoterPtacAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD-LSSmOrange
Plasmid#37129DepositorInsertLSSmOrange
TagsHisExpressionBacterialMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoteraraBAvailable sinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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LB322 (Bcl-2 from ATG to -3934)
Plasmid#15381DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterialMutationBcl-2 fragment from ATG to -3934 with both P1 and…Available sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pAKgfplux1
Plasmid#14083DepositorInsertBacterial luciferase
ExpressionBacterialAvailable sinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX-2T-Rac1-wt
Plasmid#12977DepositorAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody
Plasmid#199390PurposeBacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tagDepositorInsertanti-ALFA nanobody
Tags14xHis-Avi-SUMOEuExpressionBacterialPromoterT5-LacOAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHN924
Plasmid#178885PurposeExpression of GST-His8-HRV3CDepositorInserthuman rhinovirus 3C protease
TagsGST, His8ExpressionBacterialPromoterT7Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
COVID-SARS2 NSP13
Plasmid#159614PurposeBacterial expression plasmid for COVID-SARS2 NSP13 helicaseDepositorInsertCovid-SARS2 Nsp13
TagsHis6-Zb-TEVExpressionBacterialMutationCodon-optimized for E. coli expressionPromoterT7 - LacOAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDNA2.0 6H-TEV-KRAS
Plasmid#111849PurposeExpresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialPromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDawn-sfGFP
Plasmid#107741PurposeExpresses superfolder GFP under the control of a blue-light transcriptional regulatorDepositorInsertsuperfolder GFP
ExpressionBacterialPromoterpLambdaAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSAM_AraC
Plasmid#91569PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.DepositorInsertAraC/PBAD
ExpressionBacterialAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCold-I-Dsup
Plasmid#90021PurposeTo express His-tagged Dsup in bacteriaDepositorInsertDsup
TagsHisExpressionBacterialPromotercspAAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-CalfluxVTN
Plasmid#83926PurposeBioluminescent Reporter of Intracellular CalciumDepositorInsertCalfluxVTN
ExpressionBacterialMutationNonePromoterT7Available sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-scFv-T
Plasmid#67843PurposepET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.DepositorInsertantibody 1D8 light kappa chain
TagsHis-tagExpressionBacterialPromoterT7 promoterAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-1
Plasmid#61736PurposeStreptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNADepositorPublicationInsertssSpCas9
tracrRNA
crRNA cassette
UseCRISPRExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsIAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAP01
Plasmid#59981PurposeThe function of this material is to facilitate the capture and expression of bacterial gene clusters.DepositorTypeEmpty backboneUseSynthetic Biology; Transformation-associated reco…TagsnoExpressionBacterial and YeastPromoternoAvailable sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSUMO2
Plasmid#52259PurposeBacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2DepositorTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by