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pTS117 His14-Avi-45xGS-anti GFP nanobody

Plasmid

#199370

Purpose

Bacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-GFP nanobody

Use

Tags

14xHis-Avi

Expression

Bacterial

Mutation

WT

Promoter

T5-LacO

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET19-TdTwt

Plasmid

#207466

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

TdTwt (Dntt Mouse)

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pmal_c5x_RNAse_Inhib

Plasmid

#153314

Purpose

Bacterial expression of an RNase inhibitor

Depositor

Philippa Marrack, Drew Endy

Insert

RNAse Inhibitor

Use

Tags

Maltose Binding Protein

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBbE0k-RFP

Plasmid

#35365

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 21, 2012

Availability

Academic Institutions and Nonprofits only

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pet30b-5M SrtA

Plasmid

#51140

Purpose

expression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activity

Depositor

Hidde Ploegh

Article

Shi et al Proc Natl Acad Sci U S A. 2014 Jul 15;11

Insert

S.aureus SrtA 5M

Use

Tags

6x His

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T

Promoter

T7

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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Ecoli cpGFP

Plasmid

#215713

Purpose

Expresses cpGFP in Ecoli

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

cpGFP

Use

Tags

6xHis

Expression

Bacterial

Mutation

circularly permuted GFP

Promoter

T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pREDTAI

Plasmid

#51627

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Tags

Expression

Bacterial

Mutation

Promoter

trc

Available since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pExp-NusA

Plasmid

#112571

Purpose

Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tag

Depositor

Marko Hyvönen

Type

Empty backbone

Use

Tags

8His and Strep tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-EsCas13d

Plasmid

#108303

Purpose

Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

EsCas13d

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Mutation

Promoter

lac

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-mCherry-CNA35

Plasmid

#61607

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with mCherry

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

mCherry and CNA35

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCRISPR

Plasmid

#42875

Purpose

A crRNA expression plasmid for targeting a specific sequence.

Depositor

Luciano Marraffini

Article

Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

CRISPR-BsaI

Use

CRISPR; E.coli

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pSIP403

Plasmid

#122028

Purpose

Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

Depositor

Lars Axelsson, Geir Mathiesen

Article

Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

Insert

gusA

Use

Tags

Expression

Bacterial

Mutation

Promoter

sppA

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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p404TEF1

Plasmid

#15972

Depositor

Nicolas Buchler, Fred Cross

Insert

TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

TEF1 promoter is between SacI and XbaI restrictio…

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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SpySwitch

Plasmid

#184225

Purpose

Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

SpySwitch

Use

Tags

His6

Expression

Bacterial

Mutation

E77A mutation prevents isopeptide bond formation,…

Promoter

T7

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBAV1K-T5-gfp

Plasmid

#26702

Depositor

Ichiro Matsumura

Article

Bryksin et al PLoS One. 2010 . 5(10):e13244.

Insert

T5lacOlacOeGFP biobrick

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pOlyA(WT)

Plasmid

#132391

Purpose

Ostreolysin A for sensing sphingomyelin/cholesterol complexes

Depositor

Arun Radhakrishnan

Article

Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

Insert

Ostreolysin A wild-type

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: C62S, C94S, S151C

Promoter

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBEST-OR2-OR1-Pr-araC, pBAD-TetR, pBAD-TetO1-deGFP-ssrA

Plasmid

#45789

Depositor

Richard Murray

Inserts

deGFP
Tetracycline repressor
araC

Use

Synthetic Biology

Tags

ssrA degradation tag

Expression

Bacterial

Mutation

Promoter

OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-6xHis-MBP-TEV site-TadA8.20

Plasmid

#194702

Purpose

Expresses N-terminal MBP fused TadA8.20 in bacteria

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

TadA8.20

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Promoter

T7 promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCasKP-apr

Plasmid

#117231

Purpose

Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b-RFND

Plasmid

#195339

Purpose

Expresses Engineered BrCas12b in Bacteria

Depositor

Piyush Jain

Article

Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis and 6xHis, MBP, TEV cleavage site

Expression

Bacterial

Mutation

R160E, F208W, N524V, D868V

Promoter

T7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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