32,771 results
-
Plasmid#153312PurposeBacterial expression of MMLV reverse transcriptaseDepositorInsertMMLV Reverse Transcriptase
Tags10x HisExpressionBacterialAvailable SinceJune 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVHed02
Plasmid#82352PurposeBxb1/TP901 temporal logic gate with mKate2 and sfGFP on low-copy plasmidDepositorInsertsmKate2 RFP
Superfolder GFP
Temporal logic gate with TP901 and Bxb1 attachment sites
UseSynthetic BiologyExpressionBacterialAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIT5_KP_LacI
Plasmid#173780PurposeBacterial genomic integration vector, LacI repressorDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huHkCas12a
Plasmid#174683PurposeExpresses 6xHis-MBP tagged HkCas12aDepositorArticleInserthuHkCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHAExpressionBacterialPromoterT7Available SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 V416T
Plasmid#81040Purposemammalian expression of LOV V416TDepositorInsertLOV2 V416T
TagsmCherryExpressionBacterial, Insect, and Mamm…MutationV416TAvailable SinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
His6-SMT3-SopB
Plasmid#183676PurposeRecombinant Salmonella Typhimurium SopBDepositorInsertsopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
TagsHis6-S. cerevisiae SMT3(2-101)ExpressionBacterialPromoterT7Available SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
His6-SMT3-SopB(C460S)
Plasmid#183677PurposeRecombinant Salmonella Typhimurium SopB (catalytically inactive)DepositorInsertsopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
TagsHis6-S. cerevisiae SMT3(2-101)ExpressionBacterialMutationC460SPromoterT7Available SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET 28 full-length human PARP1
Plasmid#192635PurposeExpresses human PARP1 with an N-terminal His-tagDepositorAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC-U63-tgRNA-Rev
Plasmid#112811PurposegRNA cloning vector for expressing 2-6 gRNAs based on tgFE design in DrosophilaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
AB-GFP
Plasmid#72203Purposescreen AB42 variants for aggregationDepositorAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEMG-GRIS
Plasmid#225134PurposeFor genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMGC
Plasmid#112812PurposePCR template vector for amplifying gRNA(F+E)-OstRNA fragmentDepositorInsertgRNAcore(F+E)-tRNA
ExpressionBacterialAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTP1122
Plasmid#104159PurposeBacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)DepositorInsertAnti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUPD3_FULLPRM_tetR_Ptet
Plasmid#243913PurposeGoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, including ribosome binding site.DepositorInserttetR and PtetA
ExpressionBacterialAvailable SinceJan. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pET28a:His-MBP-KD
Plasmid#237520PurposeRecombinant protein expressionDepositorInsertRwt4_kinase
ExpressionBacterialMutationKinase region of RWT4Available SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LSSmGFP-TEV-SAV
Plasmid#191200PurposeFusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInsertLSSmGFP & Streptavidin fusion
Tags6xHis and Streptavidin core domain, amino acids 1…ExpressionBacterialPromoterT7 promoterAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_EGFP_NbALFA
Plasmid#136626PurposePlasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusionDepositorInsertEGFP-NbALFA
TagsHisx10 and StrepExpressionBacterialPromoterT7Available SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
BRCA1 (1-304)
Plasmid#12645DepositorAvailable SinceJuly 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-sfGFP-FLAG:Kan
Plasmid#211833PurposePlasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.DepositorInsertsfGFP-FLAG-Kan
ExpressionBacterialMutationNAAvailable SinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET264-pUC 24xMS2V6 Loxp KANr Loxp
Plasmid#104393PurposeYeast endogenous mRNA taggingDepositorInsert24xMS2V6
ExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pM1s3AsG
Plasmid#137921PurposeE. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expressionDepositorInsertSuperfolder GFP
UseSynthetic Biology; For use with e. coli nissle in…ExpressionBacterialPromoterJ23101Available SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_25bpLinker
Plasmid#157788PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_25bp linker
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD25
Plasmid#231547PurposeL-arabinose inducible expression of heterologous genes, pBAD24-based backbone with KanR selective markerDepositorTypeEmpty backboneExpressionBacterialPromoterpBADAvailable SinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AlkB
Plasmid#228218PurposeExpress wild type AlkB protein in E. coli BL21(DE3)DepositorInsertAlpha-ketoglutarate-dependent Dioxygenase (alkB Synthetic)
TagsHis tagExpressionBacterialMutationcodon optimizedPromoterT7Available SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
-
His6-pAG-RTase
Plasmid#214932PurposeExpress fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systemsDepositorInsertfusion protein of protein A/G and MMLV reverse transcriptase (25-497)
TagsHis6ExpressionBacterialMutationMMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…PromoterT7 promoterAvailable SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pG2K
Plasmid#71742PurposeModular shuttle vector for Geobacillus and E. coliDepositorTypeEmpty backboneUseSynthetic Biology; Shuttle vectorExpressionBacterialAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-PheRSa-PheRSb
Plasmid#224364PurposeExpress PheRS protein for his-tagged purificationDepositorAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF2
Plasmid#228776PurposeProtein expression plasmid for YTH domain of YTHDF2 in E.Coli.DepositorAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a hMDH2
Plasmid#204230PurposeBacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).InserthMDH2 (MDH2 Human)
Tags6X HisExpressionBacterialMutationDoes not include transit peptide and includes a M…PromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only