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33,128 results

Showing: 7561 - 7580 of 33128 results

  2. pJJS006

    Plasmid
    #188329
    Purpose
    PCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Article
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    Linker::mNeonGreen::mIAA7
    Expression
    Bacterial
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188329-plasmid-map-sequence-id-370757

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  3. pJJS008

    Plasmid
    #188331
    Purpose
    PCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Article
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    Linker::wrmScarlet::mIAA7
    Expression
    Bacterial
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188331-plasmid-map-sequence-id-370747

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  6. pCJR01

    Plasmid
    #187888
    Purpose
    Escherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.
    Depositor
    Gaylen Uhlich
    Article
    Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144
    Insert
    DNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    187888-plasmid-map-sequence-id-368412

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  7. pBlueKan+cysMPro

    Plasmid
    #187879
    Purpose
    Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.
    Depositor
    Gaylen Uhlich
    Article
    Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    CysM
    Available Since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    187879-plasmid-map-sequence-id-368417

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  14. 6xHis-MBP-huBsCas12a

    Plasmid
    #174671
    Purpose
    Expresses 6xHis-MBP tagged BsCas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huBsCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    174671-plasmid-map-sequence-id-364005

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  16. rpoDs366C

    Plasmid
    #129688
    Purpose
    Expression of Sigma 70 S366C
    Depositor
    Jeff Gelles
    Article
    Mumm et al bioRxiv 2020
    Insert
    rpoD (rpoD E. coli)
    Tags
    His6/thrombin
    Expression
    Bacterial
    Mutation
    C132S C291S C295S S366C
    Available Since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    129688-plasmid-map-sequence-id-270569

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  20. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

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Showing: 7561 - 7580 of 33128 results