30,875 results

1. pWaldo-GFPe_PepTSo

   Plasmid

   #58334

   Purpose

   Expresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged protein

   Depositor

   So Iwata, Simon Newstead

   Article

   Newstead et al EMBO J. 2011 Jan 19;30(2):417-26. doi: 1

   Insert

   PepTso
   

   Tags

   GFP-His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pRC3EGFP

   Plasmid

   #193790

   Purpose

   Expresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   EGFP
   

   Expression

   Bacterial

   Promoter

   PR promoter

   Available Since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTE1062

   Plasmid

   #172356

   Purpose

   Detection of Streptomyces coelicolor butyrolactones (SCB) by kanamycin bioassay. Streptomyces genomic integrative vector.

   Depositor

   Eriko Takano

   Article

   Biarnes-Carrera et al Methods Mol Biol. 2018;1673:117-128. doi

   Inserts

   scbR (SCO6265 )
   kasOp
   

   Use

   Synthetic Biology; Integrative into streptomyces …

   Expression

   Bacterial

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension

   Plasmid

   #176542

   Purpose

   Bacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locus

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Inserts

   MBP tag
   TnpB
   

   Tags

   3' extension derived from endogenous locus a…

   Expression

   Bacterial

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUS252y

   Plasmid

   #191834

   Purpose

   Expresses fuYFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuYFP to place in other plasmid backbones.

   Depositor

   Nicholas Coleman

   Insert

   fuYFP
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pET26b-Granzyme A (S-A)

   Plasmid

   #8825

   Depositor

   Judy Lieberman

   Article

   Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

   Insert

   Human Granzyme A inactive (GZMA Human)
   

   Tags

   (His)6

   Expression

   Bacterial

   Mutation

   changed Serine 184 to Alanine

   Available Since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

7. 5WCI (MYST1)

   Plasmid

   #101269

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   MYST1 (KAT8 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 2, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pKPY514

   Plasmid

   #62598

   Purpose

   Encodes Thr251Gly-EcPheRS Under IPTG-Inducible (PT5) Control

   Depositor

   David Tirrell

   Article

   Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

   Insert

   Thr251Gly-EcPheRS
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Thr251Gly

   Promoter

   T5

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSW002-Pc

   Plasmid

   #108234

   Purpose

   Broad host bacterial expression vector with constitutive Pc promoter.

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   Pc promoter
   

   Expression

   Bacterial

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBbdCas9S_P0526-sgRNAmreB

    Plasmid

    #149654

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30, P0526

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. AlkB-D135T

    Plasmid

    #160433

    Purpose

    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr

    Depositor

    Tao Pan

    Article

    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

    Insert

    E. coli AlkB
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 135 to Threonine; deleted t…

    Promoter

    T7

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTW195b63

    Plasmid

    #136931

    Purpose

    Directed evolution of SpCas11

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    gIII(N)-npuN
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-4T-3-SpSuc1

    Plasmid

    #162558

    Purpose

    Expresses GST-tagged S.pombe Suc1 in E.coli

    Depositor

    Iain Hagan

    Article

    Singh et al Methods Mol Biol. 2021;2329:123-142. doi

    Insert

    Schizosaccharomyces pombe Suc1 (suc1 Fission Yeast)
    

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-SENP6 (catalytic domain)

    Plasmid

    #16359

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP6 catalytic domain (SENP6 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 628 to 1112 catalytic domain of human …

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pPEP1

    Plasmid

    #61046

    Purpose

    BglFusion compatible integration vector for Streptococcus pneumoniae

    Depositor

    Jan-Willem Veening

    Article

    Sorg et al ACS Synth Biol. 2014 Jun 4.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPBC-G3-tdT

    Plasmid

    #62814

    Purpose

    pPBC-G3-tdT expresses PiggyBac inverted terminal repeat-flanked GCaMP3 and tdTomato under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-GCaMP3-IRES-tdTomato-ITR
    

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAN3944

    Plasmid

    #74702

    Purpose

    Circuit plasmid: 0x1C

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    Circuit 0x1C
    

    Expression

    Bacterial

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBXNPH3

    Plasmid

    #110098

    Purpose

    E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag and 3C cleavage site

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Type

    Empty backbone

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. katushka-FMDV-P2A-pTRIP

    Plasmid

    #163522

    Purpose

    Used to construct lentivirus to express katushka in mammalian cells with option for a second insert

    Depositor

    Thomas Weber

    Insert

    stopless Katushka-P2A
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-His6-SUMO-cSrc-SH2-AviTag

    Plasmid

    #214230

    Purpose

    Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Li et al Elife. 2023 Mar 16;12:e82345. doi: 10.75

    Insert

    cSrc kinase SH2 domain (SRC Human)
    

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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