30,735 results

1. pLVX-Tight-Puro_Sox10

   Plasmid

   #46536

   Purpose

   A lentiviral vector for the expression of mouse Sox10 under doxycyline control.

   Depositor

   Paul Tesar

   Article

   Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

   Insert

   Sox10 (Sox10 Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFNR5

   Plasmid

   #149483

   Purpose

   Broad-Host range expression vector, RSF1010 origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   NahR(AM)-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PSalTTC

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_Pro-IL-33

   Plasmid

   #214342

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL33 (IL33 Human)
   

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28b-zDjCas13d-His

   Plasmid

   #234481

   Purpose

   Plasmid for bacterial expression and purification of DjCas13d protein (zebrafish codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

   Insert

   DjCas13d
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. araBAD-BCD20-ClpP-BCD17-ClpX

   Plasmid

   #153301

   Purpose

   Expresses clpX and clpP in E.Coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Inserts

   clpX (clpX Escherichia coli)
   clpP (clpP Escherichia coli)
   

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTcI38-Wasabi

   Plasmid

   #86117

   Purpose

   mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI38

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TcI38
   mWasabi
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T, M1V, L65S, K68R, F115L, D126G, D188G

   Promoter

   pR/pL and pR/pL, LacI

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBWB162

   Plasmid

   #140634

   Purpose

   pBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1

   Depositor

   Keith Tyo

   Article

   Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

   Insert

   mCherry
   

   Expression

   Bacterial

   Available Since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

   Plasmid

   #199371

   Purpose

   Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   anti-ALFA nanobody
   

   Tags

   14xHis-Avi

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T5-LacO

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNCS mOrange

   Plasmid

   #91764

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Orange (mOrange)

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Insert

   monomeric Orange
   

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

    Plasmid

    #181792

    Purpose

    Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC18T-mini-Tn7T-Gm-ecfp

    Plasmid

    #65030

    Purpose

    GmR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    ecfp
    

    Expression

    Bacterial

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. spNW25

    Plasmid

    #173484

    Purpose

    Expresses spNW25 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW25
    

    Tags

    Histag

    Expression

    Bacterial

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC18R6K-mini-Tn7T

    Plasmid

    #64958

    Purpose

    mini-Tn7 base vector with transcriptional terminator

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pNCST-avGFP

    Plasmid

    #129508

    Purpose

    Expresses avGFP (Lambert 2019 clone) constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    avGFP
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-19b_MMLV-RT

    Plasmid

    #153312

    Purpose

    Bacterial expression of MMLV reverse transcriptase

    Depositor

    Philippa Marrack, Drew Endy

    Insert

    MMLV Reverse Transcriptase
    

    Tags

    10x His

    Expression

    Bacterial

    Available Since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pAurora2

    Plasmid

    #213134

    Purpose

    Integrated transcriptional and translational control for stringently blue-light-activated gene expression

    Depositor

    Andreas Moeglich

    Article

    Ranzani et al Nucleic Acids Res. 2024 Aug 10:gkae678.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET29b_1_anti-GFP-nanobody

    Plasmid

    #210394

    Purpose

    Express anti-GFP nanobody in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    1_anti-GFP-nanobody
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pUC18T-mini-Tn7T-Gm-dsRedExpress

    Plasmid

    #65032

    Purpose

    GmR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    dsRedExpress
    

    Expression

    Bacterial

    Available Since

    Aug. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pPbU6_hdhfr/yfcu_Cas9

    Plasmid

    #216423

    Purpose

    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Expression

    Bacterial

    Available Since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDule2-Mb haloTyrRS C6

    Plasmid

    #160378

    Purpose

    C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

    Depositor

    Ryan Mehl

    Article

    Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

    Insert

    C6 HaloTyrosine tRNA synthatase
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Mammalian

    Mutation

    L270S, Y271L, N311G, C313T

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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