30,808 results

1. GFP Q157TAG

   Plasmid

   #68297

   Purpose

   Q157TAG GFP reporter

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   GFP Q157TAG
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer

   Plasmid

   #176589

   Purpose

   Endogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacer

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associate IscB (Chesapeake Bay) locus
   

   Expression

   Bacterial

   Mutation

   Truncated CRISPR array to two direct repeats, rep…

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKEW336-MBP-TEV-MbCas12a-33362

   Plasmid

   #115670

   Purpose

   Expresses MbCas12 (from M. bovoculi 33362) in bacteria via T7 RNAP, for purification

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   MbCas12a
   

   Use

   CRISPR

   Tags

   MBP-TEV

   Expression

   Bacterial

   Promoter

   T7 RNAP

   Available Since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMEXC3GH (Kan)

   Plasmid

   #110081

   Purpose

   Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. csr-1_son-1_iRFP670

   Plasmid

   #191749

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Tags

   NCU04502 promoter and iRFP670

   Expression

   Bacterial

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCOLA kiwi sensor sfGFP

   Plasmid

   #107368

   Purpose

   Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNA

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   kiwi-detecting toehold + sfGFP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX6P1-Ubl

   Plasmid

   #78712

   Purpose

   Expresses Sacsin Ubl- domain (2-85) in fusion with GST (bacterial)

   Depositor

   Kalle Gehring

   Article

   Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

   Insert

   Human Sacsin 2-85
   

   Tags

   GST-Tag

   Expression

   Bacterial

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJJH1295

   Plasmid

   #36914

   Depositor

   Jürgen Heinisch

   Article

   Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

   Insert

   3x mCherry
   

   Expression

   Bacterial, Mammalian, and Y…

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGT2

   Plasmid

   #13056

   Depositor

   Chaoyang Zeng

   Article

   Park et al Anal Biochem. 2007 Jan 1;360(1):144-5. d

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEA106

    Plasmid

    #187872

    Purpose

    Expresses mCherry under the virB promoter.

    Depositor

    Kan Wang

    Article

    Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

    Insert

    mCherry
    

    Expression

    Bacterial

    Promoter

    Agrobacterium virB promoter

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221-ARL8B

    Plasmid

    #67334

    Purpose

    hArl8b with stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL8B (ARL8B Human)
    

    Use

    Gateway entry vector

    Expression

    Bacterial

    Promoter

    None

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pAV104

    Plasmid

    #63194

    Purpose

    pRS404 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAB088i

    Plasmid

    #79216

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-TnCAD-F3
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBad-HisB-GdT

    Plasmid

    #87361

    Purpose

    Expresses GdT, a dimeric green fluorescent protein

    Depositor

    Robert Campbell

    Article

    Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

    Insert

    GdT
    

    Tags

    His-Tag

    Expression

    Bacterial

    Promoter

    AraBad

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. SIRT6

    Plasmid

    #41565

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SIRT6 (SIRT6 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    aa 3-353

    Promoter

    T7

    Available Since

    April 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pBbE1k-RFP

    Plasmid

    #35333

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Trc

    Available Since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUS252b

    Plasmid

    #191831

    Purpose

    Expresses fuGFPb constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPb to place in other plasmid backbones

    Depositor

    Nicholas Coleman

    Insert

    fuGFPb
    

    Expression

    Bacterial

    Available Since

    Jan. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pFTK004

    Plasmid

    #171276

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    PgpdA ANIA_08041 promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET41a-MED19

    Plasmid

    #15430

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED19 (Med19 Mouse)
    

    Tags

    GST and His

    Expression

    Bacterial

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

20. p2Lox

    Plasmid

    #34635

    Depositor

    Michael Kyba

    Article

    Iacovino et al Stem Cells. 2011 Oct;29(10):1580-8.

    Type

    Empty backbone

    Tags

    EGFP

    Expression

    Bacterial

    Promoter

    PGK promoter

    Available Since

    Feb. 7, 2012

    Availability

    Academic Institutions and Nonprofits only